Plant regeneration method for butterfly orchid

A technology of phalaenopsis and varieties, which is applied in the field of rapid propagation of phalaenopsis plants, can solve the problems affecting orchid quality, sales quantity and price, low seed germination rate, uneven flowering time, etc., so as to facilitate flowering control and industrialization unification Management, huge economic benefits, and the effect of reducing production costs

Inactive Publication Date: 2013-04-03
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a plant regeneration method of a Phalaenopsis variety, which solves the problem of slow plant propagation speed, low seed germination rate, serious viral disease, inconsistent...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Material collection method: Select several Phalaenopsis varieties with no diseases and insect pests introduced from Taiwan, and cut the pedicel axillary bud stem section with a length of 2 to 5 cm from the strong mother plant as the explant material;

[0026] (2) Preparation of medium:

[0027] Bud induction medium: MS +1.0mg / L BA + 1.0mg / L NAA +6.5 g / L Ag +30 g / L Su +2-2.5 g / L Ac +50 g / L coconut water +1-3 g / L Peptone, pH 5.6;

[0028] Cluster bud induction medium: 1 / 2MS +3.0mg / L BA + 0.5mg / L NAA +6.5 g / L Ag +30 g / L Su +2-2.5 g / L Ac +150 g / L coconut water +1-3 g / L Peptone, pH value is 5.6;

[0029] Bud proliferation medium: 1 / 2MS+2.0mg / L BA + 0.3g / L NAA + 6.5 g / L Ag + 30 g / L Su + 2-2.5 g / L Ac + 100 g / L Banana Juice + 1-3 g / L Hyponex1 + 1-3 g / L Peptone, pH The value is 5.6;

[0030] Rooting medium: 1 / 2 MS + 0.5mg / L IBA +0.3mg / L NAA +6.5 g / L Ag +30 g / L Su +2-2.5 g / L Ac, pH 5.6;

[0031] (3) Material treatment: Cut the flower stalks of several Phalaenopsis orchi...

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Abstract

The invention discloses a plant regeneration method for butterfly orchid, belonging to the field of butterfly orchid planting. The plant regeneration method comprises the following steps of: selecting pedicel axillary bud stems with length of 2-5 centimeters on butterfly orchid plants; carrying out induction culture on stem apexes for 2-3 weeks for gradually forming protocorms after 25-30 days; continuously splitting the protocorms to implement subculture acceleration reproduction; moving seedlings with height of one centimeter to a strong seedling culture medium to be cultured for 3-6 months; and inducing the seedlings to root for 20-25 days, and transplanting the strong seedlings with height of 8-10 centimeters, wherein the survive rate of the seedlings is higher than 95%, nursery-grown plants grow well after surviving. The plant regeneration method enables the excellent butterfly orchids to form sterile strains within a short time for implementing large-scale industrial production, can be produced yearly, has a high reproduction speed, and provides novel, fresh, fragrant and colorful flowers with various colors and consistent model. Moreover, a production technology with high technical content is provided to enterprises, so that the plants are consistent in size for facilitating control on flowering phases and uniform management of industrialization, the production costs of the enterprises are reduced, and giant economical benefits are created.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of Phalaenopsis plants, and in particular relates to a method for plant regeneration of Phalaenopsis varieties. Background technique [0002] Phalaenopsis, also known as Phalaenopsis, belongs to the genus Phalaenopsis, is a perennial single-stem epiphytic orchid, native to Asia, mainly distributed in the Philippines, the Malay Peninsula and Taiwan Province of my country. Phalaenopsis is a well-known species of cut flowers. Its plant shape is beautiful, with short stems, large leaves, one to several flower stems, arched, large flowers, and long-lasting flowering. The flower shape is like a butterfly flying, and the color is gorgeous. Its flowers are beautiful and colorful, and it is a treasure among tropical orchids. It has the reputation of "Queen of Orchids" and is one of the most widely cultivated and popular species in the orchidaceae. There are more than 530 varieties through hybrid...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 兰思仁吴少华何碧珠何官榕钟凤林林蔚
Owner FUJIAN AGRI & FORESTRY UNIV
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