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Asexual rapid propagation method for radix glycyrrhizae

A licorice, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of affecting yield and quality, affecting licorice yield, low reproduction coefficient, etc., and achieves low equipment requirements, rapid emergence, and rapid reproduction. Effect

Active Publication Date: 2016-02-24
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The practice production propagation of Radix Glycyrrhizae adopts seed seedling raising and rhizome propagation usually, but seed germination rate generally only has 5-10% (1999 such as Yu Linqing), adopts rhizome segmentation to carry out rhizome propagation, and the amount of species is large, Affects the yield of licorice, and the reproduction coefficient is low. Long-term propagation with rhizomes is susceptible to viruses, leading to germplasm degradation, seriously affecting yield and quality

Method used

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  • Asexual rapid propagation method for radix glycyrrhizae
  • Asexual rapid propagation method for radix glycyrrhizae

Examples

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Effect test

Embodiment 1

[0026] Embodiment 1: the asexual rapid propagation of Glycyrrhiza Ural

[0027] 1. Culture of sterile vaccine

[0028] Cut healthy and well-growing licorice shoots from the field to about 15 cm long, cut off the leaves, rinse with tap water for 10 minutes, soak in 0.1% carbendazim solution for 5-10 minutes, and then use tap water Wash 5 times. Cut the twigs into 5 cm long stems, soak them in 75% alcohol for 30 seconds, rinse them once with sterile water, take out the stems with tweezers and place them in 0.1% mercury chloride solution for disinfection for 10 Minutes, then rinsed with sterile water 6 times, blotted the stems with sterile paper to obtain sterilized licorice shoots, cut them into stem explants with 1 axillary bud, and inoculated them into the culture medium one by one , placed at 22±1℃, light intensity 50μmolm –2 the s –1 , Cultivate under the condition of light 12h / day, after 7 days of cultivation, it can be seen that the base of the stem section contacting ...

Embodiment 2

[0033] Embodiment 2: asexual rapid propagation of different strains of multiple licorice species

[0034] Adopting the same aseptic seedling culture, propagation and transplanting methods as in Example 1, multiple strains such as Radix Glycyrrhizae Glabrae and Glycyrrhiza inflata Batalin have also realized asexual rapid propagation, and the survival rate has reached 80%. above.

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Abstract

The invention discloses an asexual rapid propagation method for radix glycyrrhizae. The asexual rapid propagation method comprises the following steps: inoculating an explant of a radix glycyrrhizae stem section into a culture medium for cultivation to obtain an aseptic seedling; then cutting the aseptic seedling into a terminal bud explant, a stem section explant with one axillary bud and an explant with one axillary bud and a root, inoculating the explants into the same culture medium for cultivation to obtain a subculture-multiplicative aseptic seedling; and transplanting the subculture-multiplicative aseptic seedling into the culture medium to realize asexual rapid propagation for the radix glycyrrhizae. The formula of the used culture medium is that IBA with the concentration of 0.1mg / L is added to the MS culture medium, and the using amounts of KNO3 and NH4NO3 in the MS culture medium are halved respectively, and the pH value of the MS culture medium is 5.8. According to the asexual rapid propagation method, the root-inducing rate of the aseptic seedling is 100%, so that seedling emergence can be realized after 40 days, the seedling can be transplanted into a large field after 50 days, and the survival rate in the large field is higher than 80%. The asexual rapid propagation method can be suitable for rapid propagation of a plurality of excellent strain systems of a plurality of radix glycyrrhizae varieties, and has remarkable ecological, economic and social benefits in sustainable development of the radix glycyrrhizae.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rapidly asexually propagating licorice using tissue culture technology. Background technique [0002] Licorice (Glycyrrhizauralensis Fisch) is a perennial herbaceous plant of the genus Glycyrrhizae in the leguminous family. It is distributed in the warm temperate and cold temperate continental monsoon climates in the arid and semi-arid regions of northern my country, with an altitude of 250-1400 meters and an average annual temperature of 3-10°C. The stems and leaves of licorice are good fodder for livestock, and the root has important economic value. It is the first bulk of Chinese herbal medicines and is also a traditional bulk medicinal material for foreign exchange earning in my country. At the same time, licorice is also an important raw material in industries such as food, tobacco, and cosmetics. . In order to meet the production and market deman...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 吴国江李美茹
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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