Method for propagating tissue cultured millettia specisoa champ seedling

A technique of tissue culture and Niu Dali, applied in horticultural methods, botany equipment and methods, plant cells, etc., to achieve the effect of overcoming serious deficiencies

Inactive Publication Date: 2008-02-20
钦州市中医药研究所
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Problems solved by technology

However, due to the current shortage of wild cattle vigorously, it is v...

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Embodiment Construction

[0016] (1) Medium selection and hormone combination: MS was used as the basic medium, and different hormone combinations were used to prepare the following relevant medium:

[0017] Bud induction medium formula: MS+6-BA0.4mg.L -1 +IBA0.2mg.L -1 ;

[0018] Bud subculture proliferation medium formula: MS+6-BA0.6mg.L -1 +NAA0.5mg.L -1 ;

[0019] Rooting medium formula: 1 / 2MS+ABT 1 0.8mg.L -1 +IBA0.4mg.L -1 ;

[0020] All the above media were added with 3% white sugar and 0.35% agar, pH5.8.

[0021] (2) Selection and processing of explants: Collect materials on a sunny day, select the top buds and axillary buds of Niu Dali as explants, rinse them with tap water, cut them into 6-10cm buds, and place the explants on the ultra-clean workbench The body was soaked in 75% alcohol for 5 minutes, then sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with sterile water for 3 to 4 times, and soaked in sterile water for later use. Under sterile conditions, the ...

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Abstract

The invention provides a method for tissue culturing and reproducing the millettia speciosa champ germchit. By using the tissue culture method and taking the fresh apical bud and the axillary bud of the wild millettia speciosa champ as the explant, the explant is disinfected and sterilized by the 75 percent alcohol and 0.1 percent mercuric chloride solution; after that, according to different periods, different culture medium formulas are adopted respectively for the bud induction culture, bud subculture proliferation and the rooting culture to obtain the container seedling of the millettia speciosa champ, wherein, the bud induction culture medium has a formula of MS+6-BA0.4mg.L-1+IBA0.2mg.L-1+3 percent white granulated sugar+0.35 percent agar, the formula of the bud subculture proliferation culture medium is MS+6-BA0.6mg.L-1+NAA0.5mg.L-1+3 percent white granulated sugar+0.35 percent agar, and the formula of the rooting culture medium is 1/2MS+ABT10.8mg.L-1+IBA0.4mg.L-1+3 percent white granulated sugar+0.35 percent agar; moreover, when the height of the container seedling is grown about 2 cm, the container seedling is again transplanted to a seedbed of a greenhouse for culturing; after reaching over 20cm, the container seedling is the grown seedling of the millettia speciosa champ germchit.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of ox-diligent seedlings, in particular to a method for breeding and producing ox-diligent seedlings by means of tissue culture by using wild ox-diligent buds as main materials. Background technique [0002] Niu Dali comes from the dry tuber root of Milletia speciosa Champ., a beautiful Papilionaceae plant. It is also known as Sweet Niu Dali, Grilled Mountain Tiger, Dali Potato, and Mountain Lotus Root. It is recorded in "Guangxi Standards of Traditional Chinese Medicine" (1990 edition) Local medicinal materials are the main raw materials for the production of Chinese patent medicines including Zhuangyaojianshen Pills, Qianglijian Capsules, Yizhi Kangnao Pills, and Anti-Fengshi Liquid. It has the effects of tonifying deficiency and moistening lung, strengthening tendons and activating collaterals. Indications for lung heat, lung deficiency cough, tuberculosis, rheumatoid arthritis, l...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 李永华卢栋覃方明赵明惠朱开昕
Owner 钦州市中医药研究所
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