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Method of preparing syrup from enzymolysis of starch raw material

A technology for enzymatic hydrolysis of starch and starch raw materials, applied in the production of sugar, purification of sugar juice, production of sugar juice, etc., can solve the problems of insufficient utilization of nutrients, inhibiting the normal digestive function of animals, and preventing animals from absorbing nutrients. , to achieve the effect of small molecular weight, waste avoidance and high sweetness

Pending Publication Date: 2020-07-10
GUANGZHOU WISDOM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, starch raw materials also contain some non-starch polysaccharides. Non-starch polysaccharides refer to polysaccharides other than starch, mainly including cellulose, hemicellulose, pectin, etc., which are the main components of feed fibers. These fibers surround nutrients in the cell wall. , inhibit the normal digestive function of animals and prevent animals from absorbing nutrients
Therefore, the potential nutrients in the feed obtained after processing starch raw materials are not fully utilized, and the presence of non-starch polysaccharides will also lead to a decrease in the efficiency of subsequent preparation of starch sugars
The traditional preparation of starch sugar mostly uses corn starch as raw material. After liquefaction, saccharification, concentration and other processes, the residue after liquefaction contains protein, fat, ash, fiber, etc. The nutrients in the residue are not fully utilized, which will affect the follow-up saccharification process, and the residue (rich in protein, fat, non-starch polysaccharides) after extracting corn starch from corn has not been fully utilized

Method used

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  • Method of preparing syrup from enzymolysis of starch raw material
  • Method of preparing syrup from enzymolysis of starch raw material
  • Method of preparing syrup from enzymolysis of starch raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing syrup by enzymatically hydrolyzing starch raw materials, comprising the following steps:

[0028] Step (1): Enzymatic hydrolysis of slurry: Add water to pea flour to adjust to 25% slurry, adjust pH to 5, add compound enzyme 1 (cellulase 150U / g, hemicellulase 100U / g, pectinase 150U / g, α-galactosidase 50U / g), stirred at 37°C for 5h to obtain the enzymolysis solution;

[0029] Step (2): Liquefaction: Adjust the pH of the enzymolysis solution in step (1) to 6.0, raise the temperature to 97°C, add 24U / g of α-amylase, and stir for 60 minutes to obtain a liquefaction solution with DE value 18.5%;

[0030] Step (3): Separation: adjust the pH of the liquefied liquid in step (2) to 4.2, and centrifuge at a speed of 2700r / min for 3 minutes to obtain the precipitate and supernatant; wash and centrifuge, and wash the precipitate with water The centrifugation speed is 2500r / min, and the centrifugation time is 5min to obtain the residue and washing liquid; the...

Embodiment 2

[0036] A method for preparing syrup by enzymatically hydrolyzing starch raw materials, comprising the following steps:

[0037] Step (1): Enzymatic hydrolysis of slurry: add water to corn flour to make 30% slurry, adjust pH to 5.5, add compound enzyme 1 (cellulase 160U / g, hemicellulase 110U / g, pectinase 160U / g, α-galactosidase 60U / g), stirred at 50°C for 4 hours to obtain the enzymatic hydrolyzate;

[0038] Step (2): Liquefaction: Adjust the pH of the enzymolysis solution in step (1) to 6.2, raise the temperature to 93°C, add 20 U / g of α-amylase, and stir for 50 minutes to obtain a liquefaction solution with DE value 17.3%;

[0039] Step (3): Separation: adjust the pH of the liquefied liquid in step (2) to 4.5, and centrifuge at a speed of 2500r / min for 4 minutes to obtain a precipitate and supernatant; wash and centrifuge, and wash the precipitate with water 1 time and centrifuged, the centrifugal speed is 2600r / min, and the centrifugation time is 2min to obtain the residue...

Embodiment 3

[0045] A method for preparing syrup by enzymatically hydrolyzing starch raw materials, comprising the following steps:

[0046] Step (1): mixing enzymatic hydrolysis: adding water to pea flour, corn flour, and sweet potato flour (1:1:1 by weight) to form a 20% slurry, adjusting the pH to 4.8, adding compound enzyme 1 (cellulose Enzyme 100U / g, hemicellulase 80U / g, pectinase 130U / g, α-galactosidase 40U / g), stirred at 45°C for 3h to obtain an enzymolysis solution;

[0047] Step (2): Liquefaction: adjust the pH of the enzymolysis solution in step (1) to 5.6, raise the temperature to 95°C, add 11U / g of α-amylase, and stir for 40 minutes to obtain a liquefaction solution with DE value 15.8%;

[0048] Step (3): Separation: adjust the pH of the liquefied liquid in step (2) to 4.3, and centrifuge at a centrifugal speed of 2400r / min for 5 minutes to obtain a precipitate and supernatant; wash and centrifuge, and wash the precipitate with water 1 time and centrifuged, the centrifugal sp...

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Abstract

The invention relates to a method of preparing syrup from enzymolysis of a starch raw material. The method comprises the steps of slurry mixing enzymolysis, liquefaction, separation, saccharification,decolorization centrifugation, ion exchange and concentration steps, wherein enzymolysis of one or more starch raw materials into a plurality of slow-release functional sugars with different relativemolecular weights is carried out, starch polysaccharides and non-starch polysaccharides in the starch raw materials are fully utilized, and a high-quality sugar source is provided for a human body oran animal body. The residue produced in the preparation process is high in quality, and rich in protein and fat, so that waste of resources is avoided, and environmental pollution is reduced. The syrup prepared by the method is high in enzymolysis degree, the syrup DE value is greater than or equal to 80%, the content of syrup with the relative molecular mass of being less than 1000 Da is greaterthan or equal to 95%, glucose, sucrose, lactose and the like in a feed can be effectively replaced, the anti-oxidation function is achieved, the immune function is adjusted, the diarrhea rate of young piglets and the like is reduced, and the method has high social and economic benefits.

Description

technical field [0001] The invention relates to a method for preparing syrup by enzymatically decomposing starch raw materials, and belongs to the technical field of deep processing of starch products. Background technique [0002] Starch raw materials include pea flour, corn flour, wheat flour, black bean flour, yam flour, potato flour, pumpkin flour, millet flour, sweet potato flour, tapioca flour, sweet potato flour, lotus root flour, potato flour, etc. Such as sweet potato flour, the starch content is as high as 80%. Starch sugar is made from starch-containing grains, potatoes, beans, etc., through acid method, acid enzyme method or enzymatic method, including maltose, glucose, fructose syrup, etc. However, starch raw materials also contain some non-starch polysaccharides. Non-starch polysaccharides refer to polysaccharides other than starch, mainly including cellulose, hemicellulose, pectin, etc., which are the main components of feed fibers. These fibers surround nutr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/22C12P19/16C12P19/14C13K1/06C13K1/08C13K5/00C13B10/00C13B20/14C13B20/12C13B30/00
CPCC12P19/22C12P19/16C12P19/14C13K1/06C13K1/08C13K5/00C13B10/00C13B20/142C13B20/123C13B30/002
Inventor 吴世林闫方权吕勇江华峰
Owner GUANGZHOU WISDOM BIO TECH
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