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The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

This invention belongs to the field of polymer material preparation and nanomaterial processing technology, specifically relating to a method for preparing polyvinyl alcohol (PVA) nanoparticles. The method for preparing PVA nanoparticles provided by this invention includes the following steps: mixing PVA powder raw material with hot water to obtain a hot aqueous solution of PVA; adding the hot aqueous solution of PVA dropwise to pretreated acetone under stirring conditions to induce precipitation and obtain a suspension; performing solid-liquid separation on the suspension, and drying the resulting solid phase to obtain the PVA nanoparticles. This preparation method uses commercially available PVA as raw material and is simple, mild, and easily repeatable.