Bacillus pasteurii and bacillus agglutinans combined soil solidification method

A technology of Bacillus pasteurii and Bacillus, applied in the field of desert soil solidification, can solve the problems of poor effect, brittle damage, environmental pollution, etc., and achieve the effects of good bonding effect, increased cohesion, and good research and application value

Inactive Publication Date: 2019-04-05
INNER MONGOLIA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In traditional methods, chemical solidifiers are generally used to consolidate sand and soil, and most of the chemical solidifiers contain toxic chemical substances, which will cause environmental pollution problems
In order to avoid environmental pollution, green and environmentally friendly microorganisms are used to consolidate sandy soil, but the effect is not good, and there are brittle failure characteristics, and slope cracks appear

Method used

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  • Bacillus pasteurii and bacillus agglutinans combined soil solidification method
  • Bacillus pasteurii and bacillus agglutinans combined soil solidification method
  • Bacillus pasteurii and bacillus agglutinans combined soil solidification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] refer to Figure 1 to Figure 3 , a kind of bacillus pasteurianus and bacillus cementum joint soil consolidation method, comprises the following steps:

[0039] 1. Provide Bacillus Pasteurella and Bacillus cementum bacteria solutions;

[0040] Wherein, the bacillus pasteurian bacteria liquid and the bacillus cementum bacteria liquid belong to two independent liquids independently of each other. The OD600 value of the Bacillus pasteurian bacteria liquid is 0.6-0.8, and the OD600 value of the Bacillus cementum bacteria liquid is 1.0-2.0. The specific preparation method of bacillus pasteurian bacterium liquid and bacillus cementum bacterium liquid is as follows:

[0041] 1) Preparation of Bacillus pasteurian bacteria liquid;

[0042] The bacillus pasteurian bacteria liquid is obtained by inoculating the bacillus pasteurianus into No. 1 culture medium. Medium No. 1 includes yeast powder 15-20g / L, ammonium sulfate 7-10g / L and Tris 13-18g / L per liter of distilled water. S...

Embodiment 2

[0052] refer to Figure 4 , a kind of bacillus pasteurianus and bacillus cementum joint soil consolidation method, can also adopt following steps:

[0053] A. Provide mixed bacterial solution;

[0054] The mixed bacterial liquid adopts Bacillus pasteurianus and Bacillus cementum simultaneously, and the volume ratio of Bacillus pasturella and Bacillus cementum in the mixed bacterial liquid is (2:1)~(4:1). Its concrete preparation method is as follows:

[0055] The mixed bacteria solution is obtained by simultaneously inoculating Bacillus pasteurianus and Bacillus cementum into No. 3 culture medium. Described No. 3 medium comprises yeast powder, ammonium sulfate, Tris, sucrose and distilled water. In every liter of distilled water, yeast powder accounts for 23-28g, ammonium sulfate accounts for 7-10g, Tris accounts for 13-18g, and sucrose accounts for 8-12g. The specific cultivation process of the mixed bacterial solution in medium No. 3 is the same as that described in Exam...

Embodiment 3

[0060] The Bacillus cementum used in Example 1 and Example 2 is isolated and purified from the desert red clay in Inner Mongolia. Its specific separation method is as follows:

[0061] refer to Figure 5 , the isolation method of bacillus cementum comprises the following steps:

[0062] S1. Take n grams of desert soil at 120-150 cm from the desert soil in Inner Mongolia, put it into 10 n grams of sterile distilled water and shake it for 15-20 minutes, then let it stand for 12 hours, and take the supernatant for subsequent use;

[0063] S2. Inoculate the supernatant into No. 1 liquid medium, and carry out constant temperature shaking culture;

[0064] S3. According to the concentration gradient of dilution, prepare the liquid after constant temperature shaking culture in step S2 into dilution solutions of different concentrations, and apply the dilution solutions of different concentrations on the plates with No. 1 solid medium on the upper surface respectively , and then ca...

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Abstract

The invention discloses a bacillus pasteurii and bacillus agglutinans combined soil solidification method, which comprises the following steps of bacillus pasteurii bacterium liquid and bacillus agglutinans bacterium liquid providing, sand sample mineralization and sand sample solidification. The bacillus pasteurii is used for inducing calcium carbonate to be precipitated into pores of sand particles; primary solidification is performed; then, exopolysaccharides secreted by the bacillus agglutinans in the life cycle are used for being continuously filled into the pores of the sand particles for secondary solidification; the solidification capability is enhanced; good study and application values are realized. By using the bacillus pasteurii and bacillus agglutinans combined soil solidification method, inside particle connection of the solidified sand is compact; the bonding effect is better; the cohesive force is increased. After the combined soil solidification method is used for solidifying the sand, the whole strength of the solidified sand is high. In addition, the combined soil solidification method has the characteristic that the brittleness damage generated by the single soil solidification by the bacillus pasteurii is avoided.

Description

technical field [0001] The present invention relates to the technical field of desert soil solidification, in particular to a method of combining bacillus cementum and bacillus pasturianus for soil consolidation. Background technique [0002] With the growth of my country's population and the development of society, the demand for construction land is increasing. However, the area of ​​arable land in our country is getting less and less, so how to use the potential land has become an important research direction today. The desert is a land of potential use. Good control of deserts is conducive to the adjustment of industrial structure and the comprehensive development of agriculture, forestry, animal husbandry and fishery. Due to the destruction of nature by human activities, desertification is becoming more and more serious, and desertification has become a problem that almost all countries in the world must solve. [0003] To control the desert, we must first solve the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12N1/20E02D3/00C12R1/07
CPCC12N1/20C12Q1/02E02D3/00G01N2333/32
Inventor 李驰范文涵高利平
Owner INNER MONGOLIA UNIV OF TECH
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