Preparation method of probiotics for feed

A technology of probiotics and feed, applied in the field of microorganisms, can solve the problems of limited effects and high production process costs, and achieve the effects of promoting synergy, saving process costs, and enriching varieties

Pending Publication Date: 2021-01-29
黑龙江益菌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems of limited effect and high production process cost of the existing probiotics for feed, and provides a preparation method of probiotics for feed, whi...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0018] Specific embodiment one: what this embodiment records is a kind of preparation method of probiotics for feed, described method comprises the following steps:

[0019] Step 1: Prepare medium: soybean dregs 3-5%, molasses 2-6%, bran 40-50%, glucose 0.5-1%, peptone 0.5-1%, corn steep liquor 1-2.5%, urea 0.1-0.3% , Yeast extract powder 0.2-0.5%, dipotassium hydrogen phosphate 0.1-0.5%, potassium dihydrogen phosphate 0.1-0.5%, calcium carbonate 1-2%, magnesium sulfate 0.02%-1%, manganese sulfate 0.02%-1%, The balance is water (the above-mentioned percentages are mass percentages), and the above-mentioned raw materials are mixed uniformly and sterilized;

[0020] Step 2: Inoculate the starter: take the starter bacteria liquid to inoculate, the mass fraction of the inoculation amount is 3%-7%;

[0021] Step 3: Fermentation culture: at 32-37°C, with a pH of 6.5-7.2, stirring once every 6 hours, and fermenting for 24-48 hours;

[0022] Step 4: Bacteria enrichment: After the fe...

specific Embodiment approach 2

[0026] Embodiment 2: In the method for preparing probiotics for feed according to Embodiment 1, in step 1, the sterilization is high-pressure steam sterilization at a temperature of 121° C. for 20 minutes.

specific Embodiment approach 3

[0027] Specific embodiment three: the preparation method of a kind of feed probiotic described in specific embodiment one, in step two, described leavening agent is Lactobacillus plantarum, Bacillus subtilis, saccharomycete and Clostridium butyricum, each strain The ratio is 2:3:1:1.

[0028] Specific embodiment four: the preparation method of a kind of probiotics for feed described in specific embodiment three, the number of viable bacteria of each strain in the starter is 6 * 10 7 cfu / ml, the inoculation temperature is room temperature.

[0029] Embodiment 5: In the method for preparing probiotics for feed described in Embodiment 1, in step 4, the speed of the centrifugation is 4000-8000r / min, and the time is 10-20min.

[0030] Embodiment 6: The method for preparing probiotics for feed described in Embodiment 1, in step 5, the mass ratio of trehalose, skim milk powder, maltodextrin and corn starch is 2:1:1 :2.

[0031] Embodiment 7: In Embodiment 1, a method for preparing...

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Abstract

The invention discloses a preparation method of probiotics for feed, and belongs to the technical field of microorganisms. In order to solve the problems of limited effect and high manufacturing process cost of probiotics for the existing feed, the method comprises the following steps: preparing a culture medium and sterilizing; inoculating a fermenting agent; carrying out fermentation culture for24-48 hours; enriching the thalli and suspending the thalli into a bacterial suspension by using normal saline; preparing a protective agent; carrying out heat stress treatment on the bacterial suspension; and spray drying with a fluidized bed: adding the bacterial liquid added with the protective agent into a spray fluidization dryer for drying to obtain the mixed bacterial preparation. Mixed fermentation culture of the strains is carried out by utilizing the demand difference of the strains on oxygen. The probiotics for the feed are prepared on the basis of a mixed bacteria fermentation technology, a heat stress technology and a spray drying zone fluidized bed technology. Compared with a preparation method of probiotics for a traditional feed, the preparation method disclosed by the invention has the advantages that the types of the probiotics are enriched, the synergistic effect among strains is promoted, the formation of bacillus spores is promoted, and the viability of thalli isimproved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of probiotics for feed, which is applied to the production of probiotics for feed. Background technique [0002] my country is a big country in agriculture and animal husbandry, and the production and consumption of animal source food is very huge. With the improvement of living standards, the proportion of fish, poultry, meat, eggs, milk and other animal source foods in the diet continues to increase, and the proportion of meat consumption in the diet is increasing year by year. With the continuous development of intensive farming, the amount of livestock and poultry breeding has continued to increase, meeting the needs of consumers, but some problems have gradually emerged. The expansion of breeding scale and the concentration of breeding density have increased the threat of animal diseases. Therefore, enhancing animal immunity and impro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/25C12R1/125C12R1/645C12R1/145
CPCC12N1/20C12N1/16A23K10/18
Inventor 李庆华
Owner 黑龙江益菌生物科技有限公司
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