Growth-promoting biological bacterial fertilizer for saline-alkali land

A technology of biological bacterial fertilizer and saline-alkali land, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as inconvenience

Inactive Publication Date: 2018-04-20
SHANDONG SHENGJING TOURISM DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, there are obviously inconveniences and defects in

Method used

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  • Growth-promoting biological bacterial fertilizer for saline-alkali land

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Experimental program
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Effect test

Embodiment 1

[0084] A: Production of complex microbial agents

[0085]a. inoculate Trichoderma aculeatus into PDA culture fluid, and carry out liquid fermentation; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 170rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;

[0086] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;

[0087] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature 35-37 °C, ...

Embodiment 2

[0097] A: Production of complex microbial agents

[0098] a. inoculate Trichoderma aculeatus into PDA culture fluid, and carry out liquid fermentation; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 175rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;

[0099] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;

[0100] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature 35-37 °C,...

Embodiment 3

[0110] A: Production of complex microbial agents

[0111] a. Trichoderma aculeatus is inoculated into PDA culture fluid, and liquid fermentation is carried out; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 165rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;

[0112] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;

[0113] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature ...

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Abstract

The invention relates to the technical field of biological bacterial fertilizers, in particular to a growth-promoting biological bacterial fertilizer for saline-alkali land. The preparation of the fertilizer comprises the steps of: A, production of a compound microbial agent; and B: preparation of the growth-promoting biological bacterial fertilizer: mixing the compound microbial agent prepared inthe step A, turf soil, urea, a calcium magnesium phosphate fertilizer, vermiculite, potassium sulfate and attapulgite according to a proportion for 1.5 to 2 hours under stirring, performing granulation, drying and sieving to obtain the grwoth-promoting biological bacterial fertilizer for saline-alkali land. Through the above technical scheme, the compound microbial agent is obtained by performingliquid fermentation and solid fermentation to prepare Trichoderma asperellum solid inoculum, Bacillus subtilis solid inoculum and Bacillus licheniformis solid inoculum, mixing the three solid inoculaaccording to a ratio of 1:1:1 and performing complete crushing; and then the growth-promoting biological bacterial fertilizer for saline-alkali land is prepared by adding the turf soil, the urea, thecalcium magnesium phosphate fertilizer, the vermiculite, the potassium sulfate and the attapulgite into the compound microbial agent and performing mixing under stirring, granulation, drying and sieving. The growth-promoting biological bacterial fertilizer for saline-alkali land achieves a Suaeda glauca survival rate of 95% or more through a Suaeda glauca planting growth-promoting experiment in saline-alkali land.

Description

technical field [0001] The invention relates to the technical field of biological bacterial fertilizer, in particular to a growth-promoting biological bacterial fertilizer for saline-alkali land. Background technique [0002] Soil salinization is increasingly threatening the limited land resources on which human beings depend. Under the severe pressure of increasing population, dwindling cultivated land and insufficient fresh water resources, how to use large areas of saline-alkali land, desertified land and other resources to develop agriculture has become a major issue. [0003] Cultivate salt-tolerant plant varieties through agricultural biotechnology or develop and utilize economically valuable halophyte resources to improve saline soil. Therefore, the development of biological fertilizers suitable for saline-alkaline environments is an effective way to ensure that plants can fully adapt to saline environments through biological means. It is a new direction of saline s...

Claims

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Application Information

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IPC IPC(8): C05G3/00C12N1/20C12N1/14C12R1/10C12R1/125C12R1/885
CPCC05B13/02C05G3/00C12N1/14C12N1/20C05C9/00C05D1/02C05D9/00C05F11/02C05F11/08
Inventor 王胜
Owner SHANDONG SHENGJING TOURISM DEV CO LTD
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