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A kind of bacillus firmus bacterial agent and its preparation method and application

A technology of Bacillus firmus and Bacillus, applied in the field of microorganisms, can solve the problems of tobacco root damage, unsatisfactory effect, and stimulation of crop growth

Active Publication Date: 2019-08-23
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For a long time, root-knot nematode control of tobacco and other crops has generally been based on pesticides such as abamectin and thiazophos, but the use of abamectin and thiazophos will generally cause certain damage to the root system of tobacco, and the effect is not ideal ; Microbial pesticides control root-knot nematodes, which can effectively reduce the occurrence of nematodes, and can inhibit the bacteria carried by nematodes, improve the micro-ecological environment of roots, and stimulate crop growth; today, microbial control has gradually become a research hotspot in plant disease control. Research, development and utilization Microbial pesticides are undoubtedly the best way to control root-knot nematode diseases in crops such as tobacco

Method used

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  • A kind of bacillus firmus bacterial agent and its preparation method and application
  • A kind of bacillus firmus bacterial agent and its preparation method and application
  • A kind of bacillus firmus bacterial agent and its preparation method and application

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preparation example Construction

[0036] The preparation method of bacillus firmus inoculum as described above comprises the steps:

[0037] The pre-prepared Bacillus firmus seed solution is inoculated into the fermentation medium for fermentation, and the obtained fermentation solution is concentrated, dried and air-jet crushed to obtain the Bacillus firmus bacterial agent.

[0038] Preferably, the concentration is concentrated by selecting ceramic membrane filtration;

[0039] Further preferably, the pore diameter of the ceramic membrane is 0.01 μm˜0.1 μm.

[0040] Preferably, the drying is centrifugal spray drying, the inlet air temperature is 120°C-220°C, and the outlet air temperature is 60°C-90°C;

[0041] Preferably, the preparation method of the Bacillus firmus bacterial agent as described above, the fermentation medium contains yeast powder 25±4g / L, cornstarch 20±5g / L, glucose 10±2g / L, calcium chloride 3± 0.5g / L, soybean meal powder 10±5g / L, defoamer 1.4±0.2g / L, pH=8.1~8.3.

[0042] Preferably, the...

Embodiment 1

[0062] 1.1. Obtaining of Bacillus firmus seed solution

[0063] 1.1.1. Activation of Bacillus firmus strains: Use an inoculation loop to pick up the freeze-dried strains of Bacillus firmus three rings into a 250ml Erlenmeyer flask, and culture them at 35°C for 18 hours with a solid nutrient medium.

[0064] 1.1.2, Streak method solid plate culture

[0065] Incubate on a solid nutrient medium plate at 35°C for 100 hours, and a single colony grows.

[0066] 1.1.3. Test tube slant culture: solid nutrient medium test tube slant, pick the above-mentioned single colony and inoculate it on the test tube slant, culture at 35°C for 100h, until the spores are scattered.

[0067] Select better strains from the above-mentioned test tube slant, rinse the test tube slant seeds with sterile water, connect it to a 750mL Erlenmeyer flask filled with liquid nutrient medium, and cultivate it at 35°C with a shaker speed of 200r / min for 24h. The bacterial content reaches 0.6×10 9 When it is abo...

Embodiment 2

[0080] Indoor activity determination of Bacillus firmus against tobacco root-knot nematode (Meloidogyne incongnita Chitwood)

[0081] Pick the galls of root-knot nematodes from tobacco roots with severe disease, place them on the nylon omentum in a petri dish, add appropriate amount of sterile water, place them in an incubator at (25±1)°C for incubation, and collect after 48 hours The second-instar larvae are prepared into a nematode suspension, and each 1 mL suspension contains about 200 second-instar larvae for later use.

[0082] Choose 2.5×10 9 Live spores / gram Verticillium chlamydosporium (verticillium chlamydosporium) parent drug was used as the control drug. Manufacturer: Yunnan Luliang Enzyme Preparation Co., Ltd.

[0083] Will 1×10 11 Live spores / gram Bacillus firmus parent drug and 2.5×10 9 Viable spores / gram Verticillium chlamydia parent drug were dissolved with sterile water respectively to make 1.00×10 4 mg / L concentration mother liquor, for later use.

[00...

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Abstract

The invention relates to the technical field of microorganisms, and in particular to a bacillus firmus inoculant. Each gram of the inoculant contains 1x10<10-12> viable bacillus spores; and the bacillus firmus is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.1.2010. The inoculant can be used for preventing and controlling root-knot nematode disease in such crops as tobacco, banana, tomato and the like; and the inoculant is safe to human and animals, good in environmental compatibility, free from residue of micro toxicity and free from pollution, and drug resistance of harmful pest cannot be caused easily.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus firmus bacterial agent and its preparation method and application. Background technique [0002] Root-knot nematode is a kind of omnivorous plant pathogenic nematode, which mainly infects the root system of crops, making the roots swollen, deformed, chicken-claw-shaped, and the root tissue turns black and rotten. Some roots also produce spherical root knots. After infection, the plants show Symptoms of water shortage, wilting at noon, can recover in the early morning and evening, but in the later period, the plants cannot grow normally, or even die. Root-knot nematode is one of the major pathogens of food, vegetables, and economic crops in my country. At present, root-knot nematode has become a devastating disease of vegetables in protected areas in the north, and it is also a major disease of fruits and vegetables cultivated in open fields in the south. Root-k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/07
CPCA01N63/00C12N1/20
Inventor 张海华张小平李明林
Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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