Multi-functional bacterial strain and application thereof

A strain and function technology, applied in the field of biological preparations, can solve the problems of poor soil adaptability, unstable quality, and single function of biological fertilizers, and achieve the effects of improving shelf life, strong resistance to stress and reducing mortality.

Inactive Publication Date: 2009-09-09
北京易众恒科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the biological fertilizers currently on the market generally have problems such as si

Method used

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  • Multi-functional bacterial strain and application thereof
  • Multi-functional bacterial strain and application thereof
  • Multi-functional bacterial strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The property of embodiment 1 soil bacillus described in the present invention and function experiment

[0040] The soil bacillus described in the present invention is isolated from rice rhizosphere soil by the inventor, and its properties and functions are as follows:.

[0041] 1. Morphological and physiological and biochemical characteristics: G + , rod-shaped, the size is (0.4-0.6)×(1.5-3)μm; spores grow laterally, most of which are oval; Vp - , the pH of the culture solution is 7.0-7.2; grow at a pH of 5.5-9.8 + . Does not grow on anaerobic media, does not grow in NaCl above 7.5%; starch hydrolysis - , gelatin liquefaction + , breaking down casein + , contact enzyme + , oxidase - , citrate utilization - , glucose gas production - , acid production from glucose + , L-arabinose produces acid - , acid production from xylose - , mannitol produces acid + , nitrate reduction + , methyl red test + .

[0042] The detection methods used above are the classic m...

Embodiment 2

[0061] The preparation of embodiment 2 spore powder

[0062] (1) Seed activation: pick the frozen preserved bacterial liquid, streak it on a solid medium plate, and cultivate it at 30°C for 14 hours; then pick a single colony and inoculate it in the liquid medium, and culture it at 30°C and 250rpm until OD 600nm = 1.5;

[0063] (2) Spore generation: Take 0.2 mL of the bacterial solution obtained in step (1), inoculate it into a Keeri flask equipped with a solid medium, spread it evenly, and cultivate it at 30°C for 3 days until an obvious bacterial lawn is formed on the surface of the medium. get spore seeds;

[0064] (3) Inoculate the seed tank: wash the bacterial lawn with sterile water, inoculate in the sterilized seed tank, the inoculation temperature is 70°C, the fermentation parameters are set as: temperature 35°C, tank pressure 0.03MPa, ventilation rate 1:0.2(m 3 / t / h), pH 6.8, stirring speed 250rpm, incubation time 20h;

[0065] (4) Fermentation cultivation: the see...

Embodiment 3

[0069] The preparation of embodiment 3 spore powder

[0070] (1) Seed activation: pick the frozen preserved bacterial liquid, streak it on a solid medium plate, and cultivate it at 30°C for 16 hours; then pick a single colony and inoculate it in the liquid medium, and shake it at 30°C and 250rpm until OD 600nm = 1;

[0071] (2) Spore generation: Take 0.2 mL of the bacterial solution obtained in step (1), inoculate it into a Keer flask containing a solid medium, spread it evenly, and cultivate it at 30°C for 4 days until an obvious bacterial lawn is formed on the surface of the medium. get spore seeds;

[0072] (3) Inoculate the seed tank: wash the bacterial lawn with sterile water, inoculate in the sterilized seed tank, the inoculation temperature is 80°C, the fermentation parameters are set as: temperature 30°C, tank pressure 0.05MPa, ventilation rate 1:0.3(m 3 / t / h), pH 7.2, stirring speed 400rpm, incubation time 24h;

[0073] (4) Fermentation cultivation: the seed liquid...

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Abstract

The invention provides a Bacillus edaphicus CGMCC No.2315 which is high in salt tolerance, heat resistance and adversity-resistance, can secrete various phytohormones such as indopropionic acid, zeatin, gibberellin, and the like, and can control nematodes and various fungal diseases. The invention also provides a method for producing the spore powder of the Bacillus edaphicus, which effectively reduces the death rate of the core bacterial strains, enables the survival rate of the core bacterial strains to reach 93 percent above and prolongs the guarantee period of the spore powder to more than 12 months. The multi-functional bacterial strain and the production method of the spore powder thereof are suitable for producing biological organic fertilizer, ecological formula fertilizer, biological pesticide, soil improvement agent and other biological preparations.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a strain of multifunctional soil bacillus and its application. Background technique [0002] In my country, the long-term excessive application of chemical fertilizers has caused a series of problems such as soil compaction and fertility degradation. At the same time, people are increasingly concerned about pesticide and fertilizer residues in agricultural products, and green food and organic food are popular in the market. In this environment, my country's bio-fertilizer industry has developed rapidly. The annual output of bio-fertilizers has reached 4 million tons, and there are as many as 12 types of products registered with the Ministry of Agriculture, including rhizobia fertilizers, nitrogen-fixing bacteria fertilizers, and phosphorus-solubilizing bacteria. Fertilizers, potassium-dissolving fungus fertilizers, etc. [0003] However, the biological fertilizers currentl...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00C05F11/08C09K17/00A01N63/02A01P3/00A01P5/00C12R1/07
Inventor 刘旭明
Owner 北京易众恒科技有限公司
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