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Fermentation method and application of bacillus

A technology of bacillus and fermentation method, applied in the field of microorganisms, can solve the problems of underutilization of active ingredients, environmental pollution, waste of resources, etc., and achieve the effect of improving soil aggregate structure, reducing costs, and reducing investment

Active Publication Date: 2018-12-28
SHANGHAI FONDIN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main treatment methods of these wastes are to dry them as feed or discharge them directly. The active ingredients are not fully utilized, which not only causes great pollution to the environment, but also causes a waste of resources.

Method used

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  • Fermentation method and application of bacillus
  • Fermentation method and application of bacillus
  • Fermentation method and application of bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A fermentation method for bacillus, specifically comprising the steps of:

[0033] Inoculate the bacillus in the culture medium, and culture it on a shaking table at 25° C. and pH 6.5-7.5 for 45 hours. After the fermentation is completed, obtain the bacillus fermentation liquid, and spray-dry the bacillus fermentation liquid to obtain the bacillus spore powder; The shaking table speed is 180r / min.

[0034] The medium includes the following materials in parts by weight: 150 parts of yeast fermentation waste, 6 parts of sodium chloride, 3 parts of corn flour, 0.5 parts of manganese sulfate monohydrate, and 750 parts of water.

[0035] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 20 parts of urea, 30 parts of nitrogen and potassium compound fertilizer, 25 parts of ammonium dihydrogen phosphate, 400 parts of seaweed extract, humic 500 parts of Potassium Acid, 100 parts of the abov...

Embodiment 2

[0037] A fermentation method for bacillus, specifically comprising the steps of:

[0038] Bacillus was inoculated into the culture medium, and cultured on a shaker at 30° C. and pH 6.5-7.5 for 50 hours. After the fermentation was completed, a Bacillus fermentation liquid was obtained; the shaker speed was 160 r / min.

[0039] The medium includes the following materials in parts by weight: 300 parts of yeast fermentation waste, 4 parts of sodium chloride, 6 parts of corn flour, 0.3 parts of manganese sulfate monohydrate, and 800 parts of water.

[0040] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 30 parts of urea, 20 parts of nitrogen and potassium compound fertilizer, 20 parts of ammonium dihydrogen phosphate, 500 parts of seaweed extract, humic 550 parts of Potassium Acid, 150 parts of the above-mentioned bacillus spore powder that makes.

Embodiment 3

[0042] A fermentation method for bacillus, specifically comprising the steps of:

[0043] Bacillus was inoculated into the culture medium, and cultured on a shaker at 35° C. and pH 6.5-7.5 for 48 hours. After the fermentation was completed, a Bacillus fermentation broth was obtained; the shaker rotated at 120 r / min.

[0044] The medium includes the following materials in parts by weight: 230 parts of yeast fermentation waste, 3 parts of sodium chloride, 5 parts of corn flour, 0.4 parts of manganese sulfate monohydrate, and 700 parts of water.

[0045] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 25 parts of urea, 28 parts of nitrogen and potassium compound fertilizer, 30 parts of ammonium dihydrogen phosphate, 600 parts of seaweed extract, humic 600 parts of Potassium Acid, 200 parts of above-mentioned bacillus spore powders that make.

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Abstract

The invention relates to a fermentation method of bacillus. A culture medium adopted in a fermentation process comprises substances in parts by weight as follows: 150-300 parts of yeast fermentation waste, 3-6 parts of sodium chloride, 3-6 parts of corn flour, 0.3-0.5 parts of manganese sulfate monohydrate and 700-800 parts of water. The invention further relates to an application of bacillus spore powder prepared with the method. The bacillus spore powder is prepared from bacillus fermentation liquor obtained by fermentation and then compounded with other plant nutrients such as seaweed extract, finally, bio-organic fertilizer is obtained by compounding, the soil aggregate structure is greatly improved, a microbial environment of soil is improved, soil diseases are effectively prevented,and application quantity of pesticides is reduced; furthermore, investment of chemical fertilizer in China can be reduced, organic matter content of soil is obviously increased, biological activity ofsoil is improved, and the product has quite important effects of improving soil fertility, increasing crop yield, improving crop quality and enhancing stress resistance of crops.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a fermentation method of bacillus and an application thereof. Background technique [0002] Yeast is a single-celled fungus that can survive in both aerobic and anaerobic environments and is a facultative anaerobic bacteria. Yeast is the earliest single-cell microorganism used by human beings and the largest number, which can be used for brewing production. Yeasts are widely distributed in nature, and most yeasts can be isolated from sugar-rich environments, such as some fruits (grapes, apples, peaches, etc.) or plant secretions (such as cactus juice) or sugar cane. The raw materials used for fermentation are usually mainly starch, molasses or other agricultural and sideline products, and the reaction can be carried out only by adding a small amount of organic and inorganic nitrogen sources. [0003] In yeast production, sugarcane molasses is used as the ferm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/07C05G3/80
CPCC12N1/20C05B7/00C05G3/00C05G3/80C05C9/00C05F11/00C05F11/02C05F11/08C05G1/00
Inventor 项金霞张忠池王保娜丁辉吴迪范佳千骆亚陈巍张建强
Owner SHANGHAI FONDIN BIO TECH
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