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Tetracycline degrading bacterium and application thereof to livestock and poultry excrement composting treatment

A technology of tetracycline and degrading bacteria, which is applied in the field of aquaculture waste treatment, can solve the problems of few types of tetracycline-degrading microorganisms, difficulty in improving the degradation efficiency, and residues of livestock and poultry manure, so as to improve the root environment of crops, reduce tetracycline residues, and enhance The effect of nutrient absorption

Active Publication Date: 2017-05-24
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The proportion of tetracycline antibiotics produced and used in my country's veterinary antibiotics is higher than that of other veterinary antibiotics, but 30-90% of tetracycline antibiotics cannot be completely absorbed by animals, and will be excreted in the form of prototype or parent compound in livestock and poultry manure produces a large amount of residue in
Currently, there are still few types of tetracycline-degrading microorganisms, and it is urgent to supplement new strain resources, and the application methods of existing strains are slightly single, and it is difficult to improve the degradation efficiency.

Method used

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  • Tetracycline degrading bacterium and application thereof to livestock and poultry excrement composting treatment
  • Tetracycline degrading bacterium and application thereof to livestock and poultry excrement composting treatment
  • Tetracycline degrading bacterium and application thereof to livestock and poultry excrement composting treatment

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Effect test

Embodiment 1

[0037]The processing object of the present invention is fresh chicken manure, the organic materials added in the composting treatment are mushroom dregs and rice bran, and the initial tetracycline content in the used chicken manure is 60mg / Kg. The number of degrading bacteria in the tetracycline-degrading bacteria fermentation broth was 1.0×10 9 CFU / mL.

[0038] The composting process is carried out in a closed composting reactor (VTD-100), and the specific steps and process conditions are as follows:

[0039] (1) 125kg of chicken manure with a moisture content of 80%, 100kg of mushroom dregs with a moisture content of 15%, and 25kg of rice bran with a moisture content of 8% are mixed evenly with a mixer. At this time, the moisture content of the mixed stock is about 46%; Divide into 2 portions, each portion is 125kg.

[0040] (2) Add 8L of the above-mentioned tetracycline-degrading bacteria fermentation liquid to one part, mix it evenly with the composting material, and the...

Embodiment 2

[0047] The processing object of the present invention is fresh pig manure, the organic materials added in the composting treatment are mushroom dregs and rice bran, and the initial content of tetracycline in the used pig manure is 78mg / Kg. The number of degrading bacteria in the tetracycline-degrading bacteria fermentation broth was 1.0×10 9 CFU / mL.

[0048] The composting process is carried out in a closed composting reactor (VTD-100), and the specific steps and process conditions are as follows:

[0049] (1) 125kg of pig manure with a moisture content of 78%, 100kg of mushroom dregs with a moisture content of 15%, and 25kg of rice bran with a moisture content of 8% are mixed evenly with a mixer, and the moisture content of the mixed stockpiles is about 46% at this time; Divide into 2 portions, each portion is 125kg.

[0050] (2) Add 8L of the above-mentioned tetracycline-degrading bacteria fermentation liquid to one part, mix it evenly with the composting material, and the...

Embodiment 3

[0057] The processing object of the present invention is fresh chicken manure, the organic materials added in the composting treatment are mushroom dregs and rice bran, and the initial tetracycline content in the used chicken manure is 60mg / Kg. The number of degrading bacteria in the tetracycline-degrading bacteria fermentation broth was 1.0×10 9 CFU / mL.

[0058] The composting process is carried out by the trough composting process, and the oxygen supply method of this process is a combination of turning and aeration.

[0059] Taking the conventional compost without adding tetracycline-degrading bacteria as a control, adjust the mixing ratio of the compost according to the routine, so that the C / N ratio of the compost is 30:1 and the moisture content is about 55%. According to 1000kg of compost, add 50kg of clean water, further mix evenly, and push the evenly mixed material into the compost tank for composting treatment. On the 28th day, the pile was removed from the compos...

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Abstract

The invention relates to the field of culture waste treatment, in particular to a tetracycline degrading bacterium and application thereof to the livestock and poultry excrement composting treatment. The bacillus cereus can be used for tetracycline degradation; through external amplification culture, degrading bacterium liquid with the thalli number being 1.0*10<9 to 10> CFU / mL is obtained; the bacterium liquid is used in the livestock and poultry excrement composting treatment according to the solid-to-liquid ratio of a certain proportion; tetracycline residue can be effectively degraded; the ecological risk of the soil after the application of livestock and poultry excrement composting products can be favorably reduced or even eliminated.

Description

technical field [0001] The invention relates to the field of breeding waste treatment, in particular to a tetracycline-degrading bacterium identified as Bacillus cereus, and its application in composting treatment of livestock and poultry manure. Background technique [0002] The proportion of tetracycline antibiotics produced and used in my country's veterinary antibiotics is higher than that of other veterinary antibiotics, but 30-90% of tetracycline antibiotics cannot be completely absorbed by animals, and will be excreted in the form of prototype or parent compound in livestock and poultry manure produced a large amount of residue. During the application of livestock and poultry manure, the residual veterinary drug antibiotics can enter the environmental media such as soil, water and the atmosphere through various channels through soil adsorption, migration, degradation and plant absorption; they can also accumulate and migrate in the food chain, and eventually It enters...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/085
CPCC05F3/00C12N1/20C05F17/50C12N1/205C12R2001/085C05F5/00C05F5/002Y02W30/40
Inventor 黄建凤张发宝逄玉万唐拴虎付弘婷李苹
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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