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A strain of tetracycline-degrading bacteria and its application in composting livestock and poultry manure

A technology of tetracycline and degrading bacteria, which is applied in the field of aquaculture waste treatment, can solve the problems of few types of tetracycline-degrading microorganisms, difficulty in improving the degradation efficiency, and single application method, so as to improve the root environment of crops, reduce tetracycline residues, and ensure degradation. efficiency effect

Active Publication Date: 2019-05-24
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The proportion of tetracycline antibiotics produced and used in my country's veterinary antibiotics is higher than that of other veterinary antibiotics, but 30-90% of tetracycline antibiotics cannot be completely absorbed by animals, and will be excreted in the form of prototype or parent compound in livestock and poultry manure produces a large amount of residue in
Currently, there are still few types of tetracycline-degrading microorganisms, and it is urgent to supplement new strain resources, and the application methods of existing strains are slightly single, and it is difficult to improve the degradation efficiency.

Method used

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  • A strain of tetracycline-degrading bacteria and its application in composting livestock and poultry manure
  • A strain of tetracycline-degrading bacteria and its application in composting livestock and poultry manure
  • A strain of tetracycline-degrading bacteria and its application in composting livestock and poultry manure

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Experimental program
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Effect test

Embodiment 1

[0037]The treatment object of the invention is fresh chicken manure, the organic materials added in the composting treatment are mushroom residue and rice bran, and the initial content of tetracycline in the chicken manure used is 60 mg / Kg. The number of degrading bacteria in the fermentation broth of tetracycline degrading bacteria is 1.0×10 9 CFU / mL.

[0038] The composting process is carried out in a closed composting reactor (VTD-100), and the specific steps and process conditions are as follows:

[0039] (1) the chicken manure 125kg with moisture content of 80%, mushroom residue 100kg with moisture content of 15%, and rice bran 25kg with moisture content of 8% are mixed with a mixer, and the moisture content of the mixed stock is about 46% now; Divide into 2 portions, each 125kg.

[0040] (2) 8L of the above-mentioned tetracycline-degrading bacteria fermentation broth was added to one part, which was further mixed with the stacking material and then loaded into a compos...

Embodiment 2

[0047] The treatment target of the present invention is fresh pig manure, the organic materials added in the composting treatment are mushroom residue and rice bran, and the initial content of tetracycline in the used pig manure is 78 mg / Kg. The number of degrading bacteria in the fermentation broth of tetracycline degrading bacteria is 1.0×10 9 CFU / mL.

[0048] The composting process is carried out in a closed composting reactor (VTD-100), and the specific steps and process conditions are as follows:

[0049] (1) 125kg of pig manure with a moisture content of 78%, 100kg of mushroom residue with a moisture content of 15%, and 25kg of rice bran with a moisture content of 8% are mixed with a mixer, and the moisture content of the mixed stock is about 46% now; Divide into 2 portions, each 125kg.

[0050] (2) 8L of the above-mentioned tetracycline-degrading bacteria fermentation broth was added to one part, which was further mixed with the stacking material and then loaded into ...

Embodiment 3

[0057] The treatment object of the invention is fresh chicken manure, the organic materials added in the composting treatment are mushroom residue and rice bran, and the initial content of tetracycline in the chicken manure used is 60 mg / Kg. The number of degrading bacteria in the fermentation broth of tetracycline degrading bacteria is 1.0×10 9 CFU / mL.

[0058] The composting process is carried out by the trough composting process, and the oxygen supply method of this process adopts a combination of two methods of turning and aeration.

[0059] The conventional compost without tetracycline-degrading bacteria was used as a control, and the mixing ratio of the compost was adjusted conventionally so that the C / N ratio of the compost was 30:1 and the moisture content was about 55%. Add 50kg of clean water according to 1000kg of piling material, further mix evenly, and push the evenly mixed material into the compost tank for composting treatment. On the 28th day, the compost was...

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Abstract

The invention relates to the field of breeding waste treatment, in particular to a tetracycline-degrading bacterium and its application in the composting treatment of livestock and poultry manure. The Bacillus cereus can be used for the degradation of tetracycline, and the number of cells obtained by external expansion and cultivation is 1.0×10 9～10 CFU / mL degrading bacteria liquid, according to a certain proportion of solid-to-liquid ratio, the bacterial liquid is used in the composting of livestock and poultry manure, which can effectively degrade the tetracycline residues, which is conducive to reducing or even eliminating the application of livestock and poultry manure composting products Ecological risks behind the soil.

Description

technical field [0001] The invention relates to the field of aquaculture waste treatment, in particular to a tetracycline-degrading bacterium identified as Bacillus cereus, and its application in the composting treatment of livestock and poultry manure. Background technique [0002] The proportion of tetracycline antibiotics produced and used in veterinary antibiotics in my country is higher than that of other veterinary antibiotics, but 30-90% of tetracycline antibiotics cannot be completely absorbed by animals, and will be excreted in the form of prototype or parent compounds, and will be excreted in livestock and poultry feces. produced a large amount of residues. During the application of livestock and poultry manure, the residual veterinary antibiotics can enter the environmental media such as soil, water and the atmosphere through various ways through soil adsorption, migration, degradation and plant absorption; they can also accumulate and migrate in the food chain, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/085
CPCC05F3/00C12N1/20C05F17/50C12N1/205C12R2001/085C05F5/00C05F5/002Y02W30/40
Inventor 黄建凤张发宝逄玉万唐拴虎付弘婷李苹
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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