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Compositions and methods for controlling plant-parasite nematode

A composition and plant technology, applied in the direction of botany equipment and methods, biochemical equipment and methods, chemicals for biological control, etc., can solve unfriendly, expensive environment and users of nematicides, secondary metabolism Issues such as unassessed effect of plant resistance on species type traits

Inactive Publication Date: 2015-06-10
BAYER CROPSCI LP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of secondary metabolites and the identity of the enzymes involved and their role in the possible induction of plant resistance have not been assessed
Fewer and fewer traditional nematicides are available today, and many of the available nematicides are very expensive or unfriendly to the environment and users

Method used

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  • Compositions and methods for controlling plant-parasite nematode
  • Compositions and methods for controlling plant-parasite nematode
  • Compositions and methods for controlling plant-parasite nematode

Examples

Experimental program
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Embodiment

[0057] In vitro testing of reniform reniform nematodes

[0058] Preliminary tests were performed to evaluate the effect of metabolites produced by Bacillus firmus on second instar larvae and egg hatching of the nematode reniformis nematode. Eggs of reniform nematodes were extracted from cotton roots by shaking the roots in 1% NaOCl solution at 120 rpm for four minutes. The egg suspension was collected through a 25 μm sieve (8) and washed with water. The eggs were washed with streptomycin sulfate (300 mg / L) and aureomycin (12.5 mg / L) to kill bacteria, and then washed with metalaxyl (25 mL / L) and iprodione (20 mL / L) to Fungi are killed, followed by a final rinse with distilled water. For the second instar larval assay, eggs were placed in Baerman trays on a modified slide heating tray at 27°C. Three days later, the second instar larvae hatched. To obtain bacterial metabolites, make Bacillus firmus GB-126 in 50 ml of tryptone soy broth (TSB) (BACTO TM ) for four days, then...

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Abstract

An entity having anti-nematodal activity derived from Bacillus firmus bacteria selected from the group consisting of isolated biosurfactant, isolated protease enzyme, isolated amylase, isolated lipase, and isolated cellulase, is disclosed. The discosure also provides for compositions comprising the same, methods of making the same, methods of controlling plant-parasite nematode, and methods of protecting plants.

Description

[0001] Cross-references to related applications [0002] This application claims priority to US Provisional Application Serial No. 61 / 700,054, filed September 12, 2012, the entire contents of which are incorporated herein by reference. technical field [0003] An entity having anti-nematode activity derived from the Bacillus firmus bacterium and selected from the group consisting of isolated biosurfactants, isolated proteases, isolated amylases, isolated lipases and isolated cellulases is disclosed . The present disclosure also provides compositions comprising the entities, methods of making the entities, methods of controlling plant parasitic nematodes, and methods of protecting plants. Background technique [0004] Bacteria are natural antagonists of an important class of plant-parasitic nematodes. Bacteria are widely distributed, have multiple modes of action, and have a broad host range (Tian, ​​B et al.). They display multiple modes of action against nematodes, inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00A01N63/22
CPCC12N9/20C12N9/2417C12N9/2437C12N9/52C12Y301/01003C12Y302/01004C12N9/2414C12N9/48C12Y302/01001C12Y304/00A01N63/22
Inventor J·L·里格斯E·L·S·卡拉J·W·克勒珀K·K·S·劳伦斯J·D·C·鲁西
Owner BAYER CROPSCI LP
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