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Bacillus firmus, bacterium agent and application thereof

A technology of Bacillus firmus and microbial agent, which is applied in the field of microbial agent and its preparation, can solve the problems such as the absence of Bacillus firmus, achieve remarkable biocontrol effects, be environmentally friendly, and improve international competitiveness

Inactive Publication Date: 2010-06-09
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, no Bacillus firmus that can be used to control strawberry powdery mildew has been found

Method used

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  • Bacillus firmus, bacterium agent and application thereof
  • Bacillus firmus, bacterium agent and application thereof
  • Bacillus firmus, bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of SAB-1 microbial bacterial agent, carries out according to the following steps:

[0046] (1) Strain activation: the strain SAB-1 stored at -40°C was activated on LB plate medium (30°C), and a single colony was picked and propagated on LB slant medium (30°C) to obtain Inoculum solution; the components and proportions of LB plate medium and LB slant medium are: 0.5% yeast extract, 1% tryptone, 0.5% NaCl, 1% agar powder; 97% distilled water, pH7.0-7.4;

[0047] (2) Preparation of seed solution: put 100mL of LB culture solution into a 250mL Erlenmeyer flask, and sterilize with high-pressure damp heat. After the temperature drops to room temperature, insert the above-mentioned activated SAB- 1 strain inoculum, vibrated culture on a shaker with a rotation speed of 190rpm, and cultivated at 30°C for 24 hours to obtain a seed solution; wherein the components and proportions of the LB liquid medium are: yeast extract 0.5%, tryptone 1% , NaCl 0.5%, distilled wa...

Embodiment 2

[0052] This experiment was carried out in the strawberry shed at the New Station of Nonghu Road, Mancheng County, Baoding City, Hebei Province, with two rows per plot, four repetitions, and random block arrangement. During the experiment, powdery mildew occurred moderately on strawberry leaves in the whole shed. Adopt the bacillus firmus SAB-1 bacterial agent prepared in embodiment 1 to inoculate with knapsack type manual sprayer (dilute with water, contain thalline 10 8 cfu / mL); another control agent (10% Shigao water dispersible granules, commercially available, produced by Syngenta, Switzerland, sprayed with 1500 times water dilution liquid) and blank control (clear water spray); after the blank control is fully developed For the survey, the grade classification standard of strawberry powdery mildew and the calculation method of control effect refer to the "Guidelines for Field Efficacy Test of National Standard Pesticides of the People's Republic of China" (2) Part 119: Fu...

Embodiment 3

[0057] This experiment was carried out in Shed 314 of Haolong Green Technology Demonstration Park in Gaobeidian City, Hebei Province, with two rows per plot, four repetitions, and random block arrangement. During the experiment, powdery mildew occurred moderately on strawberry leaves in the whole shed. Adopt the bacillus firmus SAB-1 bacterial agent prepared in embodiment 1 to inoculate with knapsack type manual sprayer (dilute with water, contain thalline 10 8 cfu / mL); another control agent (10% Shigao water dispersible granules, commercially available, produced by Syngenta, Switzerland, sprayed with 1500 times water dilution liquid) and blank control (clear water spray); after the blank control is fully developed For the survey, the grade classification standard of strawberry powdery mildew and the calculation method of control effect refer to the "Guidelines for Field Efficacy Test of National Standard Pesticides of the People's Republic of China" (2) Part 119: Fungicides C...

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Abstract

The present invention discloses one kind of Bacillius firmus SAB-1 preserved in the preservation number of CGMCC No. 2100, and the microbial preparation prepared with Bacillius firmus SAB-1. The Bacillius firmus SAB-1 may be applied in controlling powdery mildew of strawberry, and has the features of high efficiency, broad bacteriostasis spectrum, simple use, low cost, no environmental pollution,etc.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a bacillus firmus, a microbial agent produced by using the bacillus firmus, a preparation method and an application thereof. Background technique [0002] Strawberry powdery mildew is a major disease in strawberry production. In recent years, the incidence of strawberry powdery mildew has gradually increased, especially in protected strawberry powdery mildew. When it occurs seriously, the rate of diseased leaves is more than 45%, and the rate of diseased fruits is more than 50%, which seriously affects the yield, quality and economic benefits of strawberries. (Yang Lianwei. Yantai Fruit Tree, 2005(3): 15~16). As a result, farmers call strawberry powdery mildew "the cancer of strawberries." Strawberry powdery mildew is caused by strawberry powdery mildew, which is Sphaerotheca aphanis, which belongs to Ascomycota subphylum, powdery mildew order, powdery mil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02C12R1/07
Inventor 马平李社增鹿秀云郭庆港
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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