Bacillus firmus strain and application thereof in preventing and treating anthracnose of fig and pomegranate

A technology of bacillus firmus and anthracnose, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of poor control effect, achieve significant effect, reduce use, and increase spore production

Active Publication Date: 2016-12-21
SHANDONG FOREST SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the indoor confrontation test showed that its antibacterial rates to fig and p...

Method used

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  • Bacillus firmus strain and application thereof in preventing and treating anthracnose of fig and pomegranate
  • Bacillus firmus strain and application thereof in preventing and treating anthracnose of fig and pomegranate
  • Bacillus firmus strain and application thereof in preventing and treating anthracnose of fig and pomegranate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The isolation and identification of embodiment 1 bacterial strain

[0024] 1. Separation:

[0025] Bacillus firmus of the present invention is obtained from the fig rhizosphere soil in the test nursery of Shandong Academy of Forestry Sciences by using the soil dilution separation method to separate and obtain, and the separation method is:

[0026] (1) Soil sample collection: sample 200g from the soil surface to 15cm, take several samples at one location, dry in the shade in the laboratory, mix through a coarse sieve, and take 25g for separation;

[0027] (2) Medium plate preparation: NA medium (nutrient agar medium) and PDA (potato dextrose agar medium) medium are melted, poured into sterilized petri dish respectively, cool down, make NA and PDA plate;

[0028] (3) Weigh 1g of soil sample into a sterilized test tube, add 100mL of sterile water, shake, and make 10 -2 Soil suspension, let it stand for 5 minutes, and dilute to 10 by 10 times -7 ;

[0029] (4) Take 0.5...

Embodiment 2

[0044] Example 2 Verification of indoor effects of Bacillus firmus strain Bf-01 on fig and pomegranate anthracnose

[0045] 1. Experimental method

[0046] Using the plate confrontation culture method, the fig and pomegranate anthracnose bacteria cakes (9 mm) to be tested were moved to the center of the plate, and Bf-01 strain was inoculated at 2 cm around, and cultured in a biochemical incubator at 27 ° C. Each treatment was repeated 3 times. When the control hyphae covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value and relative inhibition rate were calculated.

[0047] 2. Results

[0048] It can be seen from Table 2 that the Bf-01 strain has a strong inhibitory effect on the mycelium growth of fig and pomegranate anthracnose fungus. The colony diameters after treatment were 10.3mm and 11.6mm respectively, and the control colony diameters were 70.6mm and 72.9mm respectively , myceliu...

Embodiment 3

[0051] The preparation of embodiment 3 Bacillus firmus strain Bf-01 water-dispersible granules

[0052] NB liquid medium formula: beef extract 3g, peptone 5g, sodium chloride 5g, water 1000mL, pH 7.3.

[0053] Mass fermentation culture formula (weight ratio): soybean flour 2.5%, corn flour 3%, light calcium carbonate 0.2%, sodium chloride 0.1%, the rest is water, pH 7.3.

[0054] (1) Transfer the preserved Bacillus firmus Bf-01 to the slant of the NA test tube, and place it in an incubator at 28°C for 48-72 hours;

[0055] (2) Under sterile conditions, dilute the cultured Bacillus firmus Bf-01 with sterile water at a volume ratio of 1:10, draw 1 mL, and place it in a 250 mL Erlenmeyer flask containing NB liquid medium (100 mL), Shake culture at 28°C for 18-20 hours to obtain seed liquid;

[0056] (3) The seed solution was inoculated into a large amount of fermentation medium according to the volume ratio of 3.0%, the culture temperature was 28°C, the culture time was 24 hour...

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Abstract

The invention discloses a Bacillus firmus strain and application thereof in preventing and treating anthracnose of fig and pomegranate. The strain is Bacillus firmus Bf-01, and the collection number is CGMCC No.3039. The experiment indicates that the Bacillus firmus strain Bf-01 has the characteristics of novel strain, high efficiency, high growth speed and high spore yield, and can perform quick abundant colonization on the fig and pomegranate rhizospheres. The Bacillus firmus strain Bf-01 has obvious effects on preventing and treating anthracnose of fig and pomegranate, and the preventing and treating effects reach 78.83% and 80.10%. The fig and pomegranate can grow vigorously, no chemical injury is generated, and the fresh fruit weight gain is up to 10% or above. The Bacillus firmus strain has the advantages of high safety, high efficiency, no chemical residue and no toxic or side effect, and can reduce the consumption of chemical pesticides.

Description

technical field [0001] The invention belongs to the field of microorganism application. Specifically, the present invention relates to a strain of Bacillus firmus and its application. Background technique [0002] Fig is a deciduous shrub or tree of the genus Fig in the family Moraceae, which has been introduced and cultivated for nearly a thousand years. The fruit has a soluble solid content of 24%, sweet and delicious, and contains 18 kinds of amino acids, 8 of which are essential amino acids for human body. Figs have extremely high medicinal value, can purify the intestinal tract, and promote the proliferation of beneficial bacteria. Its anti-cancer effect has also been recognized by countries all over the world, and it is known as the "patron saint of human health in the 21st century". [0003] Pomegranate is a deciduous fruit tree, shrub or small tree of the genus Pomegranate in the Pomegranate family. It has been introduced and cultivated for nearly two thousand year...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 王清海周峰孙蕾牛赡光段春华刘幸红刘慇张淑静
Owner SHANDONG FOREST SCI RES INST
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