Microorganism useful for improving saline-alkali soil, microbial agent and microbial improver for saline-alkali soil
A microbial agent and saline-alkali soil technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of poor applicability, achieve various effects, improve soil fertility, and reduce soil salinity and pH
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Embodiment 1
[0020] Strain screening.
[0021] (1) 10 sampling points were selected in Xiesheng Village, Lijin County, Binzhou City, Shandong Province, and 3 soil samples were taken within a radius of 50 cm from each sampling point. After carefully removing stones, rhizomes and various intrusions, Mix the soil sample evenly, and sieve it with a sieve with a pore size of 2 mm, to cultivate soil microorganisms and detect basic physical and chemical properties such as pH and electrical conductivity of the soil. Configure solid medium: tryptone, 10g; yeast extract, 5g; H 2 O, 1000mL; Agar, 15g; NaCl concentration gradient is respectively 2%, 3%, 4%, 5%, 6% (mass volume ratio); Utilize 1M HCl or 2M NaOH to adjust pH value to 7,7.5,8, respectively 8.5 and 9. Take 10 g of the sieved soil samples and put them into 90 mL sterile water Erlenmeyer flasks, shake on the shaker for 30 minutes, and then serially dilute to 10 with sterile water. -2 、10 -3 、10 -4 、10 -5 , take 10 -3 、10 -4 、10 -5 ...
Embodiment 2
[0036] The preparation method of biochar adopts anaerobic temperature-controlled carbonization method, and the test raw materials are corn straw, wheat straw, rice straw and alfalfa. Clean the collected raw materials, remove surface sundries, ears, leaves, etc., then repeatedly wash and remove ash with deionized water, let it stand to dry, and then transfer it to an oven at 80°C for drying. Use a pulverizer to crush the dried powder at 25,000rpm to powder, evenly load it into a quartz boat for compaction, place it in a tube furnace, blow in nitrogen, and heat at 5°C min -1 The temperature was raised to the set temperature (300°C, 500°C, 700°C) at a constant rate, kept at a constant temperature for 4 hours, the temperature was naturally cooled to room temperature, and then taken out, and refrigerated in a dry environment for later use.
[0037] Biochar yield = (m 1 -m 2 ) / m×100%
[0038] m 1 : The mass of biochar and quartz boat produced (g);
[0039] m 2 : Quartz boat ma...
Embodiment 3
[0045] Select QR-1, QR-2 two kinds of bacterial strains and two kinds of bacterial strains are carried out seed culture respectively, be respectively 2%, 3%, 4%, 5%, 6% (mass volume ratio) with NaCl concentration gradient, pH value reaches respectively Under the conditions of 7, 7.5, 8, 8.5 and 9, the seeds of the two strains were cultured respectively, and the absorbance value at 600nm was used to represent the growth of the bacteria. The initial OD value was adjusted to 0.1 under the conditions of 30°C and 3% inoculum size. After culturing for 48 hours, compare the growth of bacteria under different culture conditions, and choose the most suitable growth conditions.
[0046] The results are shown in Table 4 (QR-1) and Table 5 (QR-2), the content of salt will affect the growth of bacterial strains, and when the salt content is high, the growth of bacterial strains will be inhibited. In the experiment, it was found that the culture medium The salt content is 4%, and when the p...
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