Preparation method of bacillus firmus bacteria powder
A technology of Bacillus firmus and bacterial powder, which is applied in the field of bacterial powder preparation to achieve the effects of reducing mortality, reducing disease incidence and increasing production
Examples
Embodiment 1
[0026] A preparation method for bacillus firmus bacteria powder, comprising the following steps:
[0027] 1. Laboratory operation
[0028] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;
[0029] (2) Medium production: making liquid medium for the production of shake flask medium;
[0030] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;
[0031] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;
[0032] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;
[0033] 2. Workshop operation
[0034] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;
[0035] (2) Feeding: Pour the accurately weighed medium into the ...
Embodiment 2
[0043] A preparation method for bacillus firmus bacteria powder, comprising the following steps:
[0044] 1. Laboratory operation
[0045] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;
[0046] (2) Medium production: making liquid medium for the production of shake flask medium;
[0047] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;
[0048] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;
[0049] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;
[0050] 2. Workshop operation
[0051] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;
[0052] (2) Feeding: Pour the accurately weighed medium into the ...
Embodiment 3
[0060] A preparation method for bacillus firmus bacteria powder, comprising the following steps:
[0061] 1. Laboratory operation
[0062] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;
[0063] (2) Medium production: making liquid medium for the production of shake flask medium;
[0064] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;
[0065] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;
[0066] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;
[0067] 2. Workshop operation
[0068] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;
[0069] (2) Feeding: Pour the accurately weighed medium into the ...
PUM
Login to View More Abstract
Description
Claims
Application Information
- IPC
- C12N1/20; C12R1/07
- CPC
- C12N1/20
- Inventors
- 戴宝; 徐景超