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Preparation method of bacillus firmus bacteria powder

A technology of Bacillus firmus and bacterial powder, which is applied in the field of bacterial powder preparation to achieve the effects of reducing mortality, reducing disease incidence and increasing production

Inactive Publication Date: 2018-11-30
SHANGDONG TENOV PESTICIDES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing effect of this species against heavy cropping needs to be further improved to reduce the disease rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for bacillus firmus bacteria powder, comprising the following steps:

[0027] 1. Laboratory operation

[0028] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;

[0029] (2) Medium production: making liquid medium for the production of shake flask medium;

[0030] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;

[0031] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;

[0032] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;

[0033] 2. Workshop operation

[0034] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;

[0035] (2) Feeding: Pour the accurately weighed medium into the ...

Embodiment 2

[0043] A preparation method for bacillus firmus bacteria powder, comprising the following steps:

[0044] 1. Laboratory operation

[0045] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;

[0046] (2) Medium production: making liquid medium for the production of shake flask medium;

[0047] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;

[0048] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;

[0049] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;

[0050] 2. Workshop operation

[0051] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;

[0052] (2) Feeding: Pour the accurately weighed medium into the ...

Embodiment 3

[0060] A preparation method for bacillus firmus bacteria powder, comprising the following steps:

[0061] 1. Laboratory operation

[0062] (1) Environmental sterilization: turn on the ultraviolet germicidal lamps in the sterile room and sterile operating table for 2 hours for sterilization;

[0063] (2) Medium production: making liquid medium for the production of shake flask medium;

[0064] (3) Sterilization: Put the culture medium, etc. into the sterilizer for sterilization, and take it out after cooling;

[0065] (4) Connecting to the shaker flask: In a sterile environment, use an inoculation loop to connect the bacteria on the inclined surface to the shaker flask;

[0066] (5) Cultivation: put the shaker flask in a shaker and incubate at 29±0.5°C for 16-18 hours;

[0067] 2. Workshop operation

[0068] (1) Empty disinfection: sterilize the scrubbed fermenter with high-temperature steam for 45 minutes;

[0069] (2) Feeding: Pour the accurately weighed medium into the ...

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PUM

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Abstract

The invention discloses a preparation method of bacillus firmus bacteria powder. The preparation method comprises the following steps that 1, laboratory operations are carried out, specifically, (1) environmental sterilization is carried out, (2) culture medium preparation is carried out, (3) sterilization is carried out, (4) inoculation to a shaking flask is carried out, and (5) culture is carried out; 2, workshop operations are carried out, specifically, (1) empty sterilization is carried out, (2) feeding is carried out, (3) sterilization of solid can is carried out, (4) cooling inoculationis carried out, (5) fermentation culture is carried out, (6) detection is carried out, and (7) spray drying is carried out. The preparation method has the following beneficial effects that bacterial powder prepared through the step reduces the mortality rate of crops, reduces the incidence rate of diseases and improves the yield of the crops.

Description

technical field [0001] The invention relates to a method for preparing bacterial powder, in particular to a method for preparing bacterial powder of Bacillus firmus. Background technique [0002] Continuous cropping, also known as continuous cropping, refers to the continuous planting of the same crop on a field. Many crops, such as legumes, melons, vegetables, strawberries and some Chinese herbal medicines, etc., are caused by plant root pathogens due to heavy cropping, resulting in plant wilt, leaf blight, viral diseases and other hazards, seriously affecting crop growth. [0003] At present, a variety of beneficial microorganisms in the probiotic stock solution are used to eliminate these pathogens, and to supplement the trace elements taken away by crops due to heavy cropping, so as to prevent and control diseases caused by continuous cropping. The microorganisms in it are screened from the soil, and have antagonistic effects on most pathogenic bacteria, and they themse...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 戴宝徐景超李纪顺窦立志邱德文徐文浩刘永红
Owner SHANGDONG TENOV PESTICIDES
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