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Bacillus firmus strain and application thereof

A technology of Bacillus firmus and strains, applied in the field of probiotics, can solve problems such as not being suitable for multiplication, and achieve the effects of enhancing anti-pathogenic infection, promoting digestion, and improving the ability of prawns to resist virus and pathogenic infection

Inactive Publication Date: 2011-03-30
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although many commercial probiotic preparations are also used in aquaculture, their strains are mostly derived from terrestrial environments or animals, and are not suitable for proliferation in the digestive tract of aquatic animals and aquaculture water. Therefore, when screening probiotics, the aquaculture water and Indigenous bacteria in the digestive tract of animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of Bacillus firmus SSL065 bacterial powder:

[0023] The preparation method of Bacillus firmus SSL065 bacterial powder is as follows: pick a small amount of bacterial strains stored at -80°C and streak on 2216E solid medium, culture at 37°C for 24 hours to obtain a single colony, then inoculate it in the seed solution and shake the flask for culture, and then put the seeds The fermented liquid is inoculated in the fermented liquid for fermentation and culture, and the fermentation temperature is controlled at 30-38°C; when the OD600 value of the fermented liquid reaches 1.5-2.0, the fermentation is terminated to obtain the fermented bacteria liquid. Take the fermentation liquid and carry out centrifugal concentration 80-100 times to obtain the bacteria sludge, add a trehalose protective agent with a mass ratio of 6% to the bacteria sludge, mix well and then freeze-dry to obtain a viable bacteria concentration higher than 10 12 CFU / gram of Bacillus firmus SSL...

Embodiment 2

[0025] Application of Bacillus firmus SSL065 bacteria powder:

[0026] 20 grams of the prepared Bacillus firmus powder and 75 grams of auxiliary protection carrier cornstarch were uniformly mixed to prepare a compound probiotic preparation.

[0027] Add this compound probiotic preparation in 10 kilograms of basic prawn feed (containing fish meal 30%, shrimp meal 10%, soybean meal 25%, peanut meal 25%, flour 3%, corn meal 3%, multivitamin 1%), Formulated as immune feed containing complex probiotic preparations. Various raw materials were crushed through a 60-mesh sieve, accurately weighed, and mixed well step by step. Sodium alginate was used as a binder, and an appropriate amount of water was added to extrude it with a small meat grinder to make pellet feed, and dried at 40°C. Dried until the moisture content is below 10%, packed in separate bags, and stored in a refrigerator at 4°C for later use.

[0028] There were more than 600 healthy Litopenaeus vannamei tails. The init...

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PUM

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Abstract

The invention relates to a bacillus firmus strain and application thereof. The bacillus firmus strain SSL065 is characterized in that the collection number is CCTCC M 2010192. The bacillus firmus strain powder SSL065 prepared from the strain can be used for enhancing the immunity level of prawn cells and body fluid. The bacillus firmus strain SSL065 of the invention is screened from alimentary canals of the prawn, is an endogenous strain, has obvious effect of improving the nonspecific immunity level of bred prawn, greatly enhances the anti-pathogenic infectivity of the bred prawn, and particularly obviously improves various biological functions such as resistance of the prawn against viral pathogenic infectivity and the like.

Description

technical field [0001] The invention belongs to the field of probiotics, and in particular relates to a strain of Bacillus firmus and an application thereof. Background technique [0002] The discovery of antibiotics has promoted the great progress of human medicine. Antibiotics are also the first choice for antibacterial and disease treatment in animal breeding industry, especially in aquaculture industry. However, with the rapid development of high-density, intensive, large-scale, and factory farming models, problems such as the spread of diseases, the wanton abuse of antibiotics, the deterioration of the breeding environment, the enhancement of pathogenic drug resistance, and drug residues in farmed animals have increasingly attracted people's attention. . It has become an urgent need for the aquaculture industry to develop and develop disease control technologies and products that are in line with the environment-friendly and sustainable development strategy. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/07A23K10/18
Inventor 宋晓玲杨冰黄倢刘莉
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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