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Vibrio alginolyticus strain and application thereof

A technology of vibrio alginolyticus and strains, applied in the field of probiotics, can solve problems such as being unsuitable for proliferation, and achieve the effects of improving non-specific immunity, enhancing infectivity, and improving the ability of prawns to resist viral pathogen infection

Inactive Publication Date: 2012-04-18
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although many commercial probiotic preparations are also used in aquaculture, their strains are mostly derived from terrestrial environments or animals, and are not suitable for proliferation in the digestive tract of aquatic animals and aquaculture water. Therefore, when screening probiotics, the aquaculture water and Indigenous bacteria in the digestive tract of animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of Vibrio alginolyticus SSL073 powder is as follows:

[0022] Pick a small amount of strains stored at -80°C and streak on 2216E solid medium, culture at 28°C for 24 hours to obtain a single colony, then inoculate in the seed liquid for shaking flask culture, and then inoculate the seed liquid in the fermentation liquid for fermentation. The fermentation temperature is controlled at 25-28°C; when the OD600 value of the fermentation broth reaches 1.6, the fermentation is terminated; the fermentation broth is obtained; the fermentation broth is centrifugally concentrated 20-50 times to obtain a cloudy broth. According to 1 / 5-1 / 10 of the volume of the bacterial turbid solution, add the lysate to resuspend the bacteria, digest at 37°C for 24 hours; 300w, 10s / 10s ultrasonic crushing for 20 minutes, and the broken bacteria solution is spray-dried (air inlet and outlet) temperature respectively at 195° C. and 85° C.) to prepare Vibrio alginolyticus SSL07...

Embodiment 2

[0024] Application of Vibrio alginolyticus SSL073 powder:

[0025] Mix 10 grams of Vibrio alginolyticus powder and 85 grams of auxiliary protection carrier cornstarch evenly to prepare a compound probiotic preparation.

[0026] Add this compound probiotic preparation to 10 kilograms of basic prawn feed raw materials (containing 30% of fish meal, 10% of shrimp meal, 25% of soybean meal, 25% of peanut meal, 3% of flour, 3% of corn meal, and 1% of multivitamin) , formulated as immune feed containing disease-resistant microbial preparations. Various raw materials were crushed through a 60-mesh sieve, accurately weighed, and mixed well step by step. Sodium alginate was used as a binder, and an appropriate amount of water was added to extrude it with a small meat grinder to make pellet feed, and dried at 40°C. Dried until the moisture content is below 10%, packed in separate bags, and stored in a refrigerator at 4°C for later use.

[0027] More than 800 healthy Litopenaeus vanname...

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Abstract

The invention relates to a vibrio alginolyticus strain and application thereof, in particular to the Vibrio alginolyticus SSL073 strain with the collection number of CCTCC M 2010193. The Vibrio alginolyticus SSL073 powder prepared from the strain can be used for enhancing the immunization level of prawn cells and body fluid. The Vibrio alginolyticus SSL073 strain is screened from prawn alimentarycanal, is an endogenic strain, has multiple biological functions of remarkably improving the nonspecific immunization level of cultured prawn, greatly enhancing the pathogen infection resistance of the cultured prawn, particularly remarkably improving the capability of resisting pathogen infection of the prawn and the like.

Description

technical field [0001] The invention belongs to the field of probiotics, and in particular relates to a strain of Vibrio alginolyticus and an application thereof. Background technique [0002] The discovery of antibiotics has promoted the great progress of human medicine. Antibiotics are also the first choice for antibacterial and disease treatment in animal breeding industry, especially in aquaculture industry. However, with the rapid development of high-density, intensive, large-scale, and factory farming models, problems such as the spread of diseases, the wanton abuse of antibiotics, the deterioration of the breeding environment, the enhancement of pathogenic drug resistance, and drug residues in farmed animals have increasingly attracted people's attention. . It has become an urgent need for the aquaculture industry to develop and develop disease control technologies and products that are in line with the environment-friendly and sustainable development strategy. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/63A23K10/18A23K40/00A23K50/80
Inventor 宋晓玲刘莉黄倢杨冰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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