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Saline-alkali soil modified microbial inoculum and preparation method thereof

A microbial agent and saline-alkali soil improvement technology, applied to fertilizers made from biological waste, fertilizer mixtures, applications, etc., can solve problems such as unstable effects, achieve fast reproduction, improve adsorption and ion exchange capabilities, and stabilize strong reproductive effect

Inactive Publication Date: 2018-05-11
菏泽金正大生态工程有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The above existing microbial agents for improving saline-alkali soil are composed of many different strains, which will inevitably produce flora competition and antagonism between the strains. Instability due to geographical influence

Method used

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  • Saline-alkali soil modified microbial inoculum and preparation method thereof
  • Saline-alkali soil modified microbial inoculum and preparation method thereof
  • Saline-alkali soil modified microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 A kind of saline-alkali soil improved microbial bacterial agent and preparation method thereof, the steps are as follows:

[0053] (1) Preparation of Bacillus megaterium seed solution

[0054] A. Cultivate a single colony of Bacillus megaterium HB preserved at low temperature on an LB plate medium at 37° C., pick a single colony and culture it on an LB slant medium at 37° C. for 18 hours to obtain an activated bacterial strain;

[0055] B. Use a sterile inoculation loop to scrape a loop of the activated bacterial strain in step A and inoculate it into 50ml of liquid seed medium, and cultivate it at 35°C for 20 hours to obtain a Bacillus megaterium bacterium liquid, the number of live bacteria in the bacterium liquid is 1.5×10 9 CFU / ml.

[0056] (2) Preparation of pretreatment mixture

[0057] In parts by mass, take 40 parts of soybean meal powder, 20 parts of straw powder, 20 parts of wheat bran, 15 parts of distiller's grains, and 5 parts of corn flour, ...

Embodiment 2

[0063] Embodiment 2 A kind of saline-alkali soil improved microbial bacterial agent and preparation method thereof, the steps are as follows:

[0064] (1) Preparation of Bacillus megaterium seed solution

[0065] A. Cultivate a single colony of Bacillus megaterium strain HB preserved at low temperature on an LB plate medium at 37° C., pick a single colony and culture it on an LB slant medium at 37° C. for 24 hours to obtain an activated bacterial strain;

[0066] B. Use a sterile inoculation loop to scrape a circle of activated bacterial strains in step A and inoculate them into 50ml liquid seed culture medium, and cultivate them at 36°C for 22 hours to obtain Bacillus megaterium seed liquid, the number of viable bacteria in the liquid is 2.1×10 9 CFU / ml.

[0067] (2) Preparation of pretreatment mixture

[0068] In parts by mass, take 45 parts of soybean meal powder, 20 parts of straw powder, 10 parts of wheat bran, 15 parts of distiller's grains, and 10 parts of corn flour,...

Embodiment 3

[0074] Embodiment 3 A kind of saline-alkali soil improved microbial bacterial agent and preparation method thereof, the steps are as follows:

[0075] (1) Preparation of Bacillus megaterium seed solution

[0076] A. Cultivate a single colony of Bacillus megaterium strain HB preserved at low temperature on an LB plate medium at 37° C., pick a single colony and culture it on an LB slant medium at 37° C. for 24 hours to obtain an activated bacterial strain;

[0077] B. Use a sterile inoculation loop to scrape a loop of the activated bacterial strain in step A and inoculate it into 50ml liquid seed medium, and cultivate it at 37°C for 24 hours to obtain a Bacillus megaterium bacterium liquid, the number of live bacteria in the bacterium liquid is 1.1×10 9 CFU / ml.

[0078] (2) Preparation of pretreatment mixture

[0079] In parts by mass, take 65 parts of soybean meal powder, 25 parts of straw powder, 20 parts of wheat bran, 35 parts of distiller's grains, and 15 parts of corn fl...

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Abstract

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.

Description

technical field [0001] The invention relates to a saline-alkali soil-improving microbial bacterial agent and a preparation method thereof, belonging to the technical field of fertilizers. Background technique [0002] Saline-alkali soil is a general term for saline soil and alkaline soil. The salinization of land is mostly caused by unreasonable irrigation of farmland and excessive use of chemical fertilizers. Due to the low content of organic matter in saline-alkali soil, serious soil salinization, low soil fertility, poor physical and chemical properties, general lack of phosphorus and nitrogen, and many negative and positive ions that are harmful to crops, it is difficult for crops to emerge, which seriously affects the quality and yield of crops. . The distribution of saline-alkali soil in China is relatively wide, and the area of ​​saline-alkali land that can be used for agriculture is nearly 1.3 million hm 2 , accounting for more than 10% of China's cultivated land a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05B17/00C05G3/00C05G3/80C05D3/00C05F5/002C05F11/00C05F11/08
Inventor 邢芳芳范玲超周传志王怀利邹朋
Owner 菏泽金正大生态工程有限公司
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