Bacillus megaterium, polypeptide agricultural microbial agent thereof as well as preparation method and application of polypeptide agricultural microbial agent

Abstract

The invention relates to bacillus megaterium, a polypeptide agricultural microbial agent thereof as well as a preparation method thereof and applicationof the polypeptide agricultural microbial agent.The bacillus megaterium takes distillers' grains, soya bean meal and straw powder, which are rich in protein content, as main raw materials, and are mixed with corn flour, wheat bran, inorganic saltsand a few carbon source to reasonably compound and steam; high-yield protease bacillus subtilis is firstly utilized, the bacillus megaterium is utilized for decomposing different proteins in raw materials under a special fermentation condition to prepare the agricultural microbial agent which takes active peptides, amino acid, polysaccharides and the like as efficient ingredients; the microbial agent contains a great number of living spores, 17 free amino acids, a plurality of active peptides, and polysaccharides, and nutrient content is rich, the activity of thallus is high, and shelf life reaches two years or more; and according to the field experiment, the good effects of improving quality and increasing the yield are achieved.

Description

technical field [0001] The invention relates to a strain of Bacillus megaterium, its polypeptide agricultural microbial agent, a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. Background technique [0002] Amino acid (amino acid) is a general term for a class of organic compounds containing amino and carboxyl groups. It is the basic unit of biological functional macromolecular proteins and an organic compound containing basic amino groups and acidic carboxyl groups. Amino acids endow proteins with specific molecular structural forms, making his molecules biochemically active. As a substance that can be absorbed and utilized by plants, L-amino acid can supplement the essential amino acids of crops, stimulate and regulate the rapid growth of plants, and promote the absorption of nutrients. Improve crop quality and crop disease resistance; it can also chelate trace elements and promote the absorption and transportation ...

AI Technical Summary

Problems solved by technology

The polypeptide fertilizer obtained by acid hydrolysis has the following disadvantages: 1) it needs to be carried out in an autoclave under high temperature and high pressure conditions, which has high cost and poor safety; 2) it needs to use a large amount of acid and alkali in the production process, which is not conducive to environmental protection; 3) acid The active peptide obtained by hydrolysis of protein has a single form and limited biological activity. Most of the configurations of amino acids obtained by hydrolysis change, which cannot be absorbed and utilized by crops, and the fertilizer efficiency is low.

It uses Bacillus subtilis with high protease production, Bacillus licheniformis with high amylase production and black mold fermentation cake to produce soybean polypeptide, and produces plant polypeptide and series of products through microbial deep layer, homogeneous and liquid fermentation technology. Although the amino acid produced by the above invention- Although polypeptide liquid fertilizer has a high content of polypeptide in amino acid-polypeptide liquid fertilizer, the production process is cumbersome, the energy consumption of liquid fermentation is high, and the subsequent filtration process is added for solid-liquid separation and product extraction, and the active functional bacteria in the fermentation process are filtered out. , some incompletely degraded organic nutrients are also filtered into the solid filter residue, resulting in a large loss of active bacteria and organic nutrients, thereby reducing fertilizer efficiency

Images