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Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof

A technology of microbial agents and microbial strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems that are not suitable for the production of bacterial agents, and achieve the status of maintaining dominant strains, good antagonism, The effect of alleviating the disease of repeated cropping

Active Publication Date: 2019-05-14
KINGENTA ECOLOGICAL ENG GRP +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Streptomyces freundii used in this invention is a strain for producing tylosin by liquid fermentation, and the application is a secondary metabolite, which is not suitable for the production of bacterial agents

Method used

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  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof
  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof
  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, isolation and identification of Streptomyces fradiae (Streptomyces fradiae) XFS

[0074] 1. Isolation of Streptomyces flexneri XFS

[0075] Select the open-air garlic rhizosphere soil samples in Lanling County, Shandong Province, and use a sterilized sampling shovel to collect the garlic rhizosphere soil with good growth conditions in the same area at a depth of 5-25 cm. The samples are packed in sterilized bags and brought back to the laboratory. Store in a refrigerator at 4°C. When separating, weigh 10g of the soil sample and put it into a conical flask filled with sterile water and glass beads, and shake it for 30 minutes. After the soil sample is evenly dispersed, let it stand for 10 minutes to form a bacterial suspension with a concentration of 0.1g / mL. The bacterial suspension was diluted to obtain a concentration of 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 g / mL bacterial suspension. Pipette 0.1 mL of each gradiently diluted soil suspens...

Embodiment 2

[0086] Embodiment 2, the propagation of Streptomyces flexneri XFS bacterial classification

[0087] The cultivation method of above-mentioned Streptomyces flexneri XFS, the steps are as follows:

[0088] 1) Streptomyces freundii XFS was inoculated on the slope of the eggplant bottle, the slope of the eggplant bottle was Gaoshi No. 1 solid medium, the temperature was 30 ° C and cultured for 6 days, and the spore suspension was prepared by eluting with sterile distilled water;

[0089] 2) Inoculate the spore suspension prepared in step 1) into 200mL soybean meal powder medium for liquid culture. The inoculum size of the spore suspension is 1 eggplant bottle spore suspension, the temperature is 30°C, and the shaker speed is 200rpm. Under culture for 26 hours, that is, the mycelium in the fermentation broth grows into the logarithmic growth phase, and the Streptomyces flexneri XFS seed solution is obtained;

[0090] Described soybean meal meal culture medium, every 100 milliliter...

Embodiment 3

[0097] Embodiment 3, Streptomyces freundii XFS is to the inhibitory effect of Phytophthora garlic root rot pathogen

[0098] 1. Inoculate the bacterial block of Phytophthora root rot pathogen in the center of Gao’s No. 1 medium plate, pre-cultivate at 28°C for 1 day, inoculate Streptomyces freundii XFS at a distance of 1.5cm from the center, and cultivate in an incubator at 28°C for 5 days , observe the antibacterial zone, the antibacterial situation is as follows Figure 5 As shown, the experiment was repeated three times, and the average width of the antibacterial zone was measured to reach 15mm.

[0099] 2. Take 500 μL of the concentration of 1.0×10 7 The spore suspension of Phytophthora freundii of cfu / mL is sub-packed into a 50mL centrifuge tube, then the Streptomyces freundii XFS spore mixture prepared by 1g embodiment 2 is added in the centrifuge tube, and an equivalent amount of spore mixture is added to the control group. The solid fermentation medium prepared in Ex...

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Abstract

The invention relates to a microbial agent for repairing garlic continuous cropping soil and a preparation method and application thereof. The microbial agent for repairing the garlic continuous cropping soil is prepared from, by weight, 15-35 parts of a streptomyces fradiae XFS spore mixture, 15-25 parts of an earthworm peptide-turfy soil mixture, 20-30 parts of wormcast, 15-25 parts of diatomaceous earth, 12-20 parts of grain bran, 5-12 parts of sodium alginate and 3-10 parts of potassium fulvic acid, wherein the number of effective viable bacteria of the microbial agent is 1.2-4.5 billion / g, the content of earthworm active peptide and amino acid is 2.5-5 wt%, and the content of organic matter is 40-60 wt%. The microbial agent can be used for repairing the garlic continuous cropping soiland can also be applied to vegetables, solanaceous fruits, melons, fruit trees and other crops, the application method is a base fertilizer mode or a topdressing mode, the microbial agent can also becompounded with organic fertilizer, compound fertilizer and organic and inorganic compounded fertilizer for use, and the application amount per mu is 10-100 Kg.

Description

technical field [0001] The invention relates to a microbial agent for repairing garlic heavy cropping soil, a preparation method and application thereof, and belongs to the technical field of fertilizers. Background technique [0002] Due to the continuous cropping for many years, the occurrence area of ​​garlic diseases is increasing year by year, and has reached more than 16,000 hectares. According to the investigation and analysis of garlic continuous cropping diseases for many years, the occurrence of garlic is more serious in plots that have been continuously planted for more than 15 years. Garlic continuous cropping obstacle is not a single disease, but a combination of a series of physiological diseases and infectious diseases. In a broad sense, repeated cropping diseases of garlic include root rot, bacterial soft rot, white rot, sclerotinia, yellowing and other diseases. These diseases mainly began to erupt in the middle and late February and ended at the end of Apri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/54C05G3/80
Inventor 邢芳芳苏本玉徐春英陈海宁郑树林王敏邹朋
Owner KINGENTA ECOLOGICAL ENG GRP
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