Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof

A technology of microbial agents and microbial strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems that are not suitable for the production of bacterial agents, and achieve the status of maintaining dominant strains, good antagonism, The effect of alleviating the disease of repeated cropping

Active Publication Date: 2019-05-14
KINGENTA ECOLOGICAL ENG GRP +4
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Streptomyces freundii used in this invention is a strain for producing tylosin by liquid fermentation,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof
  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof
  • Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0073] Example 1. Separation and identification of Streptomyces fradiae XFS

[0074] 1. Isolation of Streptomyces flexneri XFS

[0075] Select the open-air garlic rhizosphere soil samples in Lanling County, Shandong Province, and use a sterilized sampling shovel to collect the garlic rhizosphere soil in the same area with a depth of 5-25 cm. The samples are packed in sterilized bags and brought back to the laboratory. Store in a refrigerator at 4°C. When separating, weigh 10 g of soil sample and put it into a triangular flask with sterile water and glass beads, shake it fully for 30 min, and after the soil sample is evenly dispersed, let it stand for 10 min to form a bacterial suspension with a concentration of 0.1 g / mL. This bacterial suspension was diluted to obtain a concentration of 10 in turn. -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 g / mL bacterial suspension. Pipette 0.1 mL of each gradient diluted soil suspension respectively, spread it on the actinomycetes se...

Example Embodiment

[0086] Embodiment 2, the propagation of Streptomyces flexneri XFS strain

[0087] The culture method of above-mentioned Streptomyces flexneri XFS, the steps are as follows:

[0088] 1) Inoculate Streptomyces flexneri XFS on the slant surface of eggplant bottle, the slant surface of eggplant bottle is Gao's No. 1 solid medium, the temperature is 30 DEG C of static culture for 6 days, and the spore suspension is prepared by eluting with sterilized distilled water;

[0089] 2) inoculate the spore suspension obtained in step 1) into 200 mL of soybean meal powder medium for liquid culture, the inoculation amount of the spore suspension is 1 eggplant bottle spore suspension, and the temperature is 30° C., the condition of the shaking table rotating speed 200rpm Cultivate for 26h, that is, the mycelium in the fermentation broth grows into the logarithmic growth phase, and the Streptomyces flexneri XFS seed liquid is obtained;

[0090] Described soybean meal powder medium, every 100 ...

Example Embodiment

[0097] Embodiment 3, the inhibitory effect of Streptomyces flexneri XFS on Phytophthora garlic root rot pathogen

[0098] 1. Inoculate the Phytophthora agaricus root rot fungus block in the center of Gao's No. 1 medium plate. After pre-incubating at 28°C for 1 day, inoculate Streptomyces flexneri XFS at a distance of 1.5cm from the center, and incubate at 28°C for 5 days. , observe the antibacterial zone, the antibacterial situation is as follows Figure 5 As shown, the experiment was repeated three times and the average width of the inhibition zone was measured up to 15 mm.

[0099] 2. Take 500 μL as a concentration of 1.0×10 7 cfu / mL of Phytophthora garlic root rot spore suspension was divided into 50mL centrifuge tubes, and then 1 g of the Streptomyces flexneri XFS spore mixture prepared in Example 2 was added to the centrifuge tube, and the control group was added with an equal amount of The solid fermentation medium prepared in Example 2 was respectively mixed with the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a microbial agent for repairing garlic continuous cropping soil and a preparation method and application thereof. The microbial agent for repairing the garlic continuous cropping soil is prepared from, by weight, 15-35 parts of a streptomyces fradiae XFS spore mixture, 15-25 parts of an earthworm peptide-turfy soil mixture, 20-30 parts of wormcast, 15-25 parts of diatomaceous earth, 12-20 parts of grain bran, 5-12 parts of sodium alginate and 3-10 parts of potassium fulvic acid, wherein the number of effective viable bacteria of the microbial agent is 1.2-4.5 billion/g, the content of earthworm active peptide and amino acid is 2.5-5 wt%, and the content of organic matter is 40-60 wt%. The microbial agent can be used for repairing the garlic continuous cropping soiland can also be applied to vegetables, solanaceous fruits, melons, fruit trees and other crops, the application method is a base fertilizer mode or a topdressing mode, the microbial agent can also becompounded with organic fertilizer, compound fertilizer and organic and inorganic compounded fertilizer for use, and the application amount per mu is 10-100 Kg.

Description

technical field [0001] The invention relates to a microbial agent for repairing garlic heavy cropping soil, a preparation method and application thereof, and belongs to the technical field of fertilizers. Background technique [0002] Due to the continuous cropping for many years, the occurrence area of ​​garlic diseases is increasing year by year, and has reached more than 16,000 hectares. According to the investigation and analysis of garlic continuous cropping diseases for many years, the occurrence of garlic is more serious in plots that have been continuously planted for more than 15 years. Garlic continuous cropping obstacle is not a single disease, but a combination of a series of physiological diseases and infectious diseases. In a broad sense, repeated cropping diseases of garlic include root rot, bacterial soft rot, white rot, sclerotinia, yellowing and other diseases. These diseases mainly began to erupt in the middle and late February and ended at the end of Apri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/54C05G3/80
Inventor 邢芳芳苏本玉徐春英陈海宁郑树林王敏邹朋
Owner KINGENTA ECOLOGICAL ENG GRP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap