Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish

A yeast culture, non-specific technology, applied in the field of animal breeding feed

Inactive Publication Date: 2017-05-24
北京中农弘科生物技术有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, most of the studies on yeast culture are based on the effects of feed additives on the growth performance of ruminants, but there are few studies on the growth performance of fish, especially the impact on non-specific immunity of fish

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish
  • Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish
  • Application of yeast culture in preparation of feed for improving fish growth and improving non-specific immunity of fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of yeast culture

[0028] 1. Activation of slant strains: inoculate the Saccharomyces cerevisiae with the preservation number of CGMCC No.2.399 frozen and preserved on the slant medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L, and agar 2%. Keep the temperature at 28°C and culture for 40 hours to obtain activated yeast strains.

[0029] 2. Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into liquid medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value is 5.6, shake at 28°C Bed culture, rotation speed 120r / min; culture 18h.

[0030] 3. Liquid aerobic fermentation: Inoculate the prepared seed liquid with 10% inoculum into a new liquid culture medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value 5.6 Carry out expanded culture in the tank, control the ventilation rate to 5L / min, the temperature is 28°C, and the fermentation time is 20h: the liq...

Embodiment 2

[0034] Embodiment 2, the preparation of yeast culture

[0035] 1. Activation of slant strains: inoculate the Saccharomyces cerevisiae with the preservation number of CGMCC No.2.399 frozen and preserved on the slant medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L, and agar 2%. Keep the temperature at 30°C and culture for 48 hours to obtain activated yeast strains.

[0036] 2. Preparation of seed liquid: Inoculate the activated yeast strain with 1 loop into liquid medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value is 6.5, and shake at a constant temperature of 30°C Bed culture, rotation speed 160r / min; culture 24h.

[0037] 3. Liquid aerobic fermentation: Inoculate the prepared seed liquid with 20% inoculation amount into a new liquid culture medium containing malt extract powder 130g / L, chloramphenicol 0.1g / L; pH value 5.6 Expanded culture was carried out in the tank, the ventilation rate was controlled at 2 L / min, the temperat...

Embodiment 3

[0041]Embodiment 3, growth performance and non-specific immune test of turbot

[0042] 1. Feed Preparation

[0043] The basic feed used in this experiment includes fish meal, soybean protein concentrate, wheat flour, α-starch, sodium carboxymethylcellulose, betaine, soybean lecithin, fish oil, soybean oil, antioxidant, premix, among which antioxidant It is a common antioxidant in fish feed, and the premix provides 5400IU vitamin A and 900IU vitamin D per kilogram of ration 3 , 48mg vitamin E, 2.4mg vitamin K 3 , 1.8mg vitamin B 1 , 7.2mg vitamin B 2 , 12mg vitamin B 6 , 0.2mg vitamin B 12 , 24mg niacinamide, 15mg calcium pantothenate, 0.06mg biotin, 120mg vitamin C, 150mg iron, 2.8mg copper, 60mg zinc, 60mg manganese, 0.75mg iodine, 0.32mg selenium, 150mg magnesium.

[0044] The addition amount of each composition of above-mentioned basal feed is shown in table 1 below.

[0045] 2. Test process

[0046] Before the experiment, the turbot was domesticated in the breeding...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a yeast culture in the preparation of feed for improving fish growth and improving non-specific immunity of fish, and the yeast culture is prepared according to a method comprising the procedures described below: activating a Saccharomyces cerevisiae (accession number of CGMCC No. 2.399) strain on an agar slant culture-medium, continuing conducting inoculation with the obtained yeast strain for preparing strain liquor, conducting aerobic fermentation followed by solid anaerobic fermentation on the strain liquor, and drying and smashing the obtained solid fermentation products to obtain the yeast culture. An experiment proves that adding the yeast culture prepared by the above process into fish feed can significantly increase the weight gain rate of fish and reduce the feed coefficient, at the same time, the yeast culture can significantly improve the activities of fish lysozyme and alkaline phosphatase, and then improve the non-specific immune level of the fish. The invention provides an important basis for improving fish production level and optimizing fish feed.

Description

technical field [0001] The invention relates to the technical field of animal breeding feed, in particular to the application of yeast culture in preparing feed for improving fish growth and nonspecific immunity of fish. Background technique [0002] Yeast culture (yeast culture, YC) refers to complex fermentation products obtained by yeast in a specific medium under certain process conditions, including cell metabolites (including polypeptides, organic acids, amino acids, nucleic acids, enzymes, etc.) , unknown growth factors, etc.), denatured medium (containing oligosaccharides, polypeptides, etc.) Nutrient substrates" to maintain the balance of various intestinal flora in the body to the maximum extent, promote normal intestinal function, improve the digestion and absorption rate of nutrients in the feed, thereby improving animal production performance. [0003] However, in the prior art, most of the studies on yeast culture are based on the effects of feed additives on ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/12
CPCY02A40/818
Inventor 刘明杨凤娟戴刚董彦君
Owner 北京中农弘科生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com