Microbial strain BF-1801 for efficiently degrading cellulose

A technology of microbial strains and strains, applied in the field of microorganisms, can solve environmental pollution and other problems, and achieve the effect of broad application prospects and good stability

Pending Publication Date: 2019-07-26
INNER MONGOLIA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of oil and coal as the main en...

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  • Microbial strain BF-1801 for efficiently degrading cellulose
  • Microbial strain BF-1801 for efficiently degrading cellulose
  • Microbial strain BF-1801 for efficiently degrading cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the culture activation of bacterial strain BF-1801

[0017] Strain BF-1801 is derived from bovine rumen, and after selection from bovine rumen, it is cultured and activated using the following fermentation medium and culture conditions.

[0018] Liquid medium (10ml): (1) Basal medium (5ml): every 50mL contains NaHCO3 0.5g, peptone 0.1g, yeast extract powder 0.1g; (2) mineral solution A (1.65mL): every 50mL contains KH 2 PO4 0.15g, (NH 4 ) 2 SO 4 0.15g, NaCl0.3g, CaCl 2 2H 2 O 0.02g, MgSO 4 ·7H 2 O 0.029g; (3) Mineral solution B (1.65mL): every 50mL contains K 2 HPO4·3H 2 O 0.198g; (3) cysteine ​​hydrochloride 0.015g, glucose 0.05g

[0019] Inject the preserved strains into the prepared liquid culture medium according to the ratio of 5%. Put into constant temperature shaking incubator, 37 ℃, 180r / min activation culture.

[0020] Solid medium: (1) Basal medium (2.5ml): every 50mL contains NaHCO 3 0.5g, peptone 0.1g, yeast extract powder 0.1g; (...

Embodiment 2

[0024] Embodiment 2: Determination of bacterial strain BF-1801 cellulase activity

[0025] 2.1 Determination method of strain BF-1801β-glucosidase enzyme activity: Take 1.0% crude enzyme solution, add 2.0ml 0.5% salicin solution, bathe in 50℃ water for 10min, add 1mlDNS reagent, fully mix and boil in boiling water bath After cooling for 5 minutes, dilute to 20ml with distilled water, measure the absorbance value OD530 at a wavelength of 530nm with a spectrophotometer, and treat the heat-inactivated crude enzyme solution as a blank in the same way.

[0026] 2.2 Determination of endoglucanase activity of strain BF-1801

[0027] Replace the substrate in 2.1 with carboxymethylcellulose sodium solution, and change the water bath time at 50°C to 30 minutes.

[0028] 2.3 Determination of exoglucanase activity of strain BF-1801

[0029] Replace the substrate in 2.1 with microcrystalline cellulose solution, and change the water bath time at 50°C to 60 minutes.

Embodiment 3

[0030] Example 3: Determination of cellulose degradation rate and hemicellulose degradation rate after fermentation by strain BF-1801 when corn stalks were used as substrate

[0031]Centrifuge the fermented liquid to remove the supernatant, put it into a vacuum dryer, weigh 0.025g of corn stalk sample and add it to the rolling tube after drying, add 250ul of 74% sulfuric acid dropwise, and put it in a water bath at 30°C for 1 hour, stirring continuously during this period. After the reaction, put the roller tube into the prepared ice bath to terminate the reaction, add 7ml of distilled water to each roller tube for dilution, and each group has three parallel samples. Then put the standard sample and the sample together in a high temperature and high pressure sterilizer for hydrolysis. After hydrolysis for 60 minutes, the temperature dropped to 100°C and the samples and standard samples were quickly taken out. After cooling at room temperature, shake the tube well (to prevent ...

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Abstract

The invention belongs to the field of microbes and particularly discloses a microbial strain BF-1801 for efficiently degrading cellulose and its application. The microbial strain BF-1801 for efficiently degrading cellulose is Bacillus firmus, its preservation number is CGMCC No.16869 (General Microbiology Center of China Microbiological Culture Collection and Management Committee). When the microbial strain BF-1801 uses corn straw as a substrate, cellulose degradation rate is 45.05%, the hemicellulose degradation rate is 31.35%; the enzyme activities of beta-glucosidase, endoglucanase and exoglucanase are 2.12 ug/ml.min, 0.617 ug/ml.min and 0.442 ug/ml.min respectively. After alkali pretreatment (10% NaOH, treatment at 80 DEG C for 180 minutes), the cellulose degradation rate and hemicellulose degradation rate of the corn straw reach 48.70% and 77.23% respectively; the cellulose degradation rate and hemicellulose degradation rate of salix psammophila reach 42.67% and 89.33% respectively. The strain can produce lactic acid and acetic acid after fermentation. The strain can carry out fermentation degradation on agricultural wastes rich in cellulose, improves the utilization rate of biomass resources, and has obvious economic and ecological significance.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a microbial strain for efficiently degrading cellulose and its application. Background technique [0002] Since the beginning of the 20th century, especially after the Second World War, oil and coal have played a huge role in the development of the world economy. However, the use of oil and coal as the main energy sources has resulted in serious environmental pollution. In addition, as non-renewable energy sources, oil and coal have limited reserves, and the crisis of energy depletion also plagues mankind. The depletion of fossil energy and the increasing global attention to environmental issues caused by greenhouse gas emissions have made energy conservation, improvement of energy efficiency, and development and utilization of renewable energy the main theme of world energy. Biomass energy is processed from biomass and is regarded as an environmentally friendly energy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/07
CPCC12N1/20C12N9/2437C12N1/205C12R2001/07
Inventor 云飞石雅丽
Owner INNER MONGOLIA UNIV OF TECH
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