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Fermentation preparation of bacillus firmus for preventing tobacco root knot nematode and applications

A technology of Bacillus firmus and tobacco root-knot nematode, applied in the fields of chemicals, applications, and tobacco cultivation for biological control, which can solve the problems of short duration, drug resistance, environmental pollution, etc., and achieve sporulation of spores High efficiency, improve soil fertility, and reduce environmental pollution

Pending Publication Date: 2021-03-16
JIANGXI SHUNQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of bacteria called stricter Bacillus (SB) helps fight against diseases caused from stubborn vegetables like crop plants or weeds. It does this because they have special properties for controlling their roots while growing on them without harmful substances being released into our environment during production processes. These unique features make these organism better at combating plant disease challenges compared with existing methods.

Problems solved by technology

The technical problem addressed by this patented method is finding an effective way to treat or manage root-nemaeid insects (Nemas) that may affect various agricultural products such as crop production without damaging them during harvesting operations.

Method used

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  • Fermentation preparation of bacillus firmus for preventing tobacco root knot nematode and applications
  • Fermentation preparation of bacillus firmus for preventing tobacco root knot nematode and applications
  • Fermentation preparation of bacillus firmus for preventing tobacco root knot nematode and applications

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Experimental program
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Effect test

Embodiment 1

[0024] A Bacillus firmus used for the control of tobacco root-knot nematode, which has been preserved in the General Microorganism Center of China Microorganism Collection Management Committee,

[0025] Classification name: Bacillus firmus; Bacillus firmus;

[0026] Deposit number: CGMCC No.20169;

[0027] Preservation address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing;

[0028] Deposit date: July 1, 2020.

[0029] A fermentative preparation of Bacillus firmus for controlling tobacco root-knot nematode, comprising the following preparation steps:

[0030] ① Bacterial cell activation, place the Bacillus firmus preserved at low temperature on a slant medium for constant temperature activation culture at 23°C for 1 day to obtain an activated strain of Bacillus firmus, the components of the slant medium are as follows in weight percentage: tryptone 2.5% , yeast extract powder 2.0%, chlorination 1.0%;

[0031] ②Preparation of seed liquid, picking activate...

Embodiment 2

[0038] Application of a Bacillus firmus bacterial agent for controlling tobacco root-knot nematode

[0039] Test site: The test site is located in Hanzhong Tobacco Planting Area, Shaanxi Province.

[0040] experiment material:

[0041] 1. 600-fold dilution of the Bacillus firmus inoculum of the present invention; 2. 600-fold dilution of the Bacillus amyloliquefaciens inoculum; 3. 600-fold dilution of the Bacillus subtilis inoculum; 4. common commercially available chemical insecticides, 5. clear water (CK); the tobacco variety is common flue-cured tobacco.

[0042] Experimental treatment: This time we controlled the number of tobacco plants in the field to 300, and each treatment method was divided into 3 small test fields, with 100 tobacco seedlings in each group, which is convenient for experimental investigation and statistics; before transplanting, we will remove the tobacco with Insect seedlings and root nodules, soak the removed tobacco seedlings in each solution at a ...

Embodiment 3

[0068] Application of a Bacillus firmus bacterial agent for controlling tobacco root-knot nematode

[0069] experiment material:

[0070] 1. 600-fold dilution of the Bacillus firmus inoculum of the present invention; 2. 600-fold dilution of the Bacillus amyloliquefaciens inoculum; 3. 600-fold dilution of the Bacillus subtilis inoculum; 4. common commercially available chemical insecticides, 5. clear water (CK); the tobacco variety is common flue-cured tobacco.

[0071] Test treatment:

[0072] Before transplanting, we will remove insect-bearing seedlings and root nodules, soak the removed tobacco seedlings in each solution at a dose of 3ml per plant, and transplant and plant the tobacco for observation after fully soaking. Aspects of data were compared statistically.

[0073] Through the above tests, it is found that a Bacillus firmus inoculum for preventing and treating root-knot nematode of the present invention can not only prevent and control root-knot nematode, but als...

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Abstract

The invention discloses fermentation preparation of bacillus firmus for preventing tobacco root knot nematode and applications. The bacillus firmus has been deposit with the China General Microbiological Culture Collection Center and assigned the accession number CGMCC No. 20169; the fermentation preparation of the bacillus firmus adopts a solid state fermentation technology; and through the adoption of the fermentation technology, a fermentation process can be accelerated, and the number of living spores in microbial agents can reach 100 billion/g. The fermentation process mainly includes asfollows: 1, performing bacterial activation to prepare activated strains; 2, performing seed liquid preparation to obtain seed liquid; 3, performing fermentation cultivation, and obtaining fermentation broth after fermentation is completed; and 4, preparing finished products, and obtaining the microbial agent. Through the preparation of the bacillus firmus, the bacillus firmus can effectively prevent tobacco root knot nematode, final prevention effects can reach 82.36%, and stable prevention effects can be realized.

Description

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Claims

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Application Information

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Owner JIANGXI SHUNQUAN BIOTECH CO LTD
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