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Microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof

A microbial organic fertilizer and strawberry anthracnose technology, which is applied in the direction of application, fertilization device, fertilizer mixture, etc., can solve the problems of insufficient stability, failure to meet actual needs, and unsatisfactory control effect of strawberry anthracnose, so as to meet nutritional needs and improve Ability to resist anthrax bacteria, value-enhancing effect

Inactive Publication Date: 2019-05-31
AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention provides a microbial organic fertilizer for preventing and treating strawberry anthracnose and its application in order to solve the problem that the current control effect of strawberry anthracnose is not ideal and stable enough to meet actual needs.

Method used

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  • Microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof
  • Microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof
  • Microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Bacterial strain is for the antibacterial experiment of anthracnose

[0030] Experimental method: using the plate confrontation method, inoculate the center of the PDA plate with 8 mm diameter of strawberry anthracnose ( C. thebromicoia ), inoculated with Bacillus Velez at a distance of 3 cm from the block ( B. velezensis ), Bacillus polymyxa ( B.polymyxa Macé ), Bacillus firmus ( B. firmus ), Trichoderma harzianum ( T. harzianum ) and Streptomyces lilacenses ( S. enissocaesilis ), the control was a plate only inoculated with Strawberry anthracnose, cultured at 28 ℃ for 3-5 days, repeated 3 times, and observed the experimental results.

[0031] For the results of the antibacterial test, see Figure 1-2 . From figure 1 It can be seen from the results that 3 strains of bacteria and 1 strain of Streptomyces have the ability to inhibit the growth of Strawberry Anthracnose, and the antibacterial effect is very obvious. From figure 2 It can be...

Embodiment 2

[0032] Embodiment 2: the ability of bacterial strain to produce IAA

[0033] Experimental method: Salkowski method was used to determine the auxin IAA secretion ability of the strain. The strain Bacillus arborii ( B. aryabhattai ) and Bacillus Zhangzhou ( B. zhangzhouensis ) were inoculated into LB medium (50ml), cultured at 28°C for 36h-48h at 150 r·min-1, collected the fermentation broth, centrifuged at 8000 r·min-1 for 2 min, removed bacteria, collected supernatant filtrate, and set aside.

[0034] Qualitative detection: Based on the color change after adding the colorimetric solution at room temperature for 15 minutes, determine whether the strain has the ability to secrete IAA; quantitative detection: add twice the volume of the colorimetric solution to the supernatant obtained after the bacterial suspension is centrifuged , after 30 min of color development in the dark at room temperature, measure the OD value at 530 nm, and calculate the auxin production of the te...

Embodiment 3

[0038] Embodiment 3: The control experiment of compound microbial agent for strawberry anthracnose

[0039] In this experiment, strawberry "Hongyan" was taken as an example to prepare organic fertilizer.

[0040] According to the growth state of cultivated strawberries, the composition of organic fertilizer is: 32 parts of sesame oil residue, 15 parts of quinoa straw, 10 parts of black fruit wolfberry residue, 10 parts of naked oat straw, 15 parts of millet straw, 7 parts of black jade powder, magnesium sulfate 1 part, 0.8 part of sugarcane molasses, 7 parts of compound microbial agent (used twice), 0.2 part of egg powder, 2 parts of polyacrylamide.

[0041] Sesame oil residue is the residue after the oil is squeezed from sesame seeds, and the residue of black fruit wolfberry is the pulp and peel after the seeds are collected.

[0042] The composite microbial agent is prepared by uniformly mixing bacteria, fungi and actinomycetes at a weight ratio of 50%: 20%: 30%, and the ba...

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Abstract

The invention belongs to the technical field of microbial pesticides, and provides a microbial organic fertilizer for preventing and treating strawberry anthracnose and application thereof in order tosolve the problems that the prevention and treatment of the strawberry anthracnose is neither ideal enough nor stable enough and can not meet actual requirements.The microbial organic fertilizer is prepared from sesame oil residues, chenopodium wheat straws, lyciumruthenicumresidues, naked oat straws, millet straws, jade powder, magnesium sulfate, sugar cane molasses, a composite microbial agent,egg powder and polyacrylamide according to a certain proportion. The composite microbial agent is prepared by mixing bacteria, fungi and actinomycetesin proportion, wherein the bacteria are the uniform mixing of bacillus velezensis, bacillus aryabhattai, bacillus Zhangzhou, bacillus polymyxa and bacillus firmus in proportion, the fungi are trichodermaharzianum, and the actinomycetes are streptomyceteslilaceous. With the microbial organic fertilizer, the ability of strawberry plants to resist anthrax bacteria is improved, the growth of strawberry root systems is promoted, the quality and yieldof strawberries are improved, the creeping stem propagation coefficient of strawberries is increased, more healthy creeping stem seedlings are obtained, and better economic benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to a microbial organic fertilizer for preventing and treating strawberry anthracnose and its application. Background technique [0002] Strawberry is a perennial herb belonging to the Rosaceae Strawberry genus. It is a fruit with high economic value. Its fruit is rich in protein and vitamins, and is soft and juicy, sweet and sour. It is known as the "Queen of Fruits" and is deeply loved by everyone. . With the increase in market demand, the area of ​​strawberry planting is also increasing, and the demand for strawberry seedlings is also increasing accordingly. [0003] Strawberry anthracnose is the main soil-borne disease of strawberry seedlings, and it is one of the main reasons for the low survival rate of strawberry transplanted in facilities. It has brought serious obstacles to strawberry production. The pathogenic bacteria of anthracnose is Anthracnose Colletotri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 陈哲梁宏黄静赵佳
Owner AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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