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Method for repairing soil at high-efficient power of bacterial to emulsify petroleum

A technology for emulsified oil and soil remediation, which is used in the restoration of polluted soil, bacteria, etc.

Inactive Publication Date: 2005-08-24
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening and application of oil-degrading bacteria producing biosurfactants for oil-contaminated soil remediation is rare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Isolation of oil-degrading bacteria with emulsifying ability: take 1 g of oil-contaminated soil and insert it into BH medium with 1% Jianghan crude oil as the sole carbon source, and culture at a constant temperature of 30°C and 180 r / min for 7 days with aerobic shaking , take different dilutions of the bacterial solution and smear the blood plate, and pick the colonies with large hemolytic circles for further separation and purification.

[0043]Using Jianghan crude oil as the sole carbon source, after enrichment culture in BH medium, multiple strains of bacteria were isolated and purified from different oil-contaminated soils with blood plate. By measuring the degree of emulsification of diesel oil by these bacteria, it was found that the strain X13-1 has a strong emulsification ability, and its emulsification rate of 1h and 24h was 80% and 75%, respectively, which was significantly higher than that reported in the literature. In addition, the bacterium gre...

Embodiment 2

[0047] Embodiment 2 is substantially the same as Example 1, but the BH medium (%) has the following changes:

[0048] MgSO 4 ·7H 2 O 0.01,

[0049] K 2 HPO 4 0.05,

[0050] K H 2 PO 4 0.05,

[0051] FeCl 3 or FeSO 4 0.004,

[0052] NH 4 NO 3 0.05,

[0053] CaCl 2 0.001,

[0054] Citric acid 0.5,

[0055] The rest is distilled water.

Embodiment 3

[0056] Embodiment 3 is basically the same as Example 1, but the BH medium (%) has the following changes:

[0057] MgSO 4 ·7H 2 O 0.03,

[0058] K 2 HPO 4 0.15,

[0059] K H 2 PO 4 0.15,

[0060] FeCl 3 or FeSO 4 0,006,

[0061] NH 4 NO 3 0.15,

[0062] CaCl 2 0.003,

[0063] Citric acid 1.50,

[0064] The rest is distilled water.

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PUM

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Abstract

A method for repairing soil at high speed and increasing the power of bacteria to emulsify petroleum includes such steps as preparing BH culture medium from MgSO4.7H2O, K2HPO4, KH4PO4, FeCl3 or FeSO4, NH4NO3, CalCl2, citric acid and water, culturing Acinetobacter calcoaceticus for 5 days while rotating at 180 rpm, adding diesel oil and fermented liquid, laying aside, cyclone oscillation, and laying aside.

Description

technical field [0001] The invention relates to an environmental protection technology, in particular to a method for cultivating bacteria. In the prior art, there is a method for repairing oil-contaminated soil by using high-efficiency bacteria strains; the present invention is a method for specifically cultivating the high-efficiency bacteria used in the method to improve its ability to emulsify petroleum. Background technique [0002] Petroleum is a complex of hundreds of chemical compounds. They are generally divided into four categories according to their properties: saturated hydrocarbons (including normal paraffins, branched alkanes and cycloalkanes), aromatic hydrocarbons (including monocyclic, bicyclic and polycyclic aromatic hydrocarbons, and cycloalkanes with alkyl side chains and fusions) ), resins (compounds composed of pyrimidine, quinoline, carbazole, thiophene, sulfoxide, amino compounds), and asphaltenes (complexes of naphthenic acid, sulfide, polyphenols, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N1/20
Inventor 刘五星骆永明
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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