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Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof

A technology of low temperature conditions and compound bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the accumulation of nitrite and nitrate, slow growth, secondary pollution of the environment, etc. problem, to achieve the effect of strong resistance, obvious effect, simple operation and management

Inactive Publication Date: 2009-09-23
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the low biological activity and slow growth rate of existing bacterial agents under low temperature conditions; when organic matter and ammonia nitrogen are treated in water, there are organic and toxic substances that are difficult to degrade, nitrite and nitrate in water accumulate, and N 2 O causes the problem of secondary pollution to the environment, and provides the composite bacteria agent and its preparation method of treating slightly polluted source water under low temperature conditions

Method used

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  • Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof
  • Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof
  • Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof

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specific Embodiment approach 1

[0010] Specific embodiment one: the composite microbial agent of treating slightly polluted source water under the low temperature condition of this embodiment consists of Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2, Bacillus megaterium (Bacillus megaterium) X2 and liquid culture medium Made; Acinetobacterlwoffii SRA10 wherein Acinetobacterlwoffii SRA10 has been preserved in the General Microbiology Center (Datun Road, Chaoyang District, Beijing City) of China Microbial Culture Collection Management Committee, and the preservation number is CGMCC No.2889, and the preservation date is January 19, 2009; Actinobacillus PHEA-2 is short rod or spherical, 1.5-2.0 μm long, 1.0-1.5 μm wide, without spores and flagella, and performs twitching movements; Bacillus megaterium X2 is rod-shaped, with rounded ends, arranged in single or short chains, During convulsions, the spores are 1.5-2.0 μm in length and 1.0-1.2 μm in width, oval in shape...

specific Embodiment approach 2

[0014] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the volume ratio of Acinetobacter lwoffiiSRA10, Acinetobacter calcoaceticus PHEA-2 and Bacillus megaterium X2 is 2:3:2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0015] Specific embodiment three: the difference between this embodiment and specific embodiment one is that every 1000 mL of liquid medium is composed of 5 g of tryptone, 3 g of yeast extract powder, 3 g of beef extract, 5 g of NaCl, and 0.9 g of Na 2 HPO 4 , 2g of KH 2 PO 4 , 5g of NH 4 Cl and the balance of water, the pH value is 7.0. Others are the same as in the first embodiment.

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Abstract

The invention relates to a composite microbial inoculum for treating micro polluted source water under the low temperature condition and a preparation method thereof, in particular to a composite microbial inoculum and a preparation method thereof, aiming at solving the problems that the existing microbial inoculum has low biological activity and slow growth velocity under the low temperature condition; when organic matter and ammonia nitrogen are treated in the water, organic and toxic substances are difficult to degrade, and nitrite and nitrate are accumulated; and secondary pollution for the environment is caused by the generated N2O. The product composite microbial inoculum is made of acinetobacter lwoffiiSRA10, acinetobacter calcoaceticus PHEA-2, bacillus megatherium X2 and fluid nutrient medium. The method comprises: 1. activation treatment is carried out on raw strain; 2. accumulation culture; 3. after being mixed according to the volume ratio, the strain is added into the fluid nutrient medium; finally, the composite microbial inoculum is obtained. The obtained composite microbial inoculum has high biological activity and fast growth and can be used for treating the micro polluted source water with remarkable effect under the low temperature condition; furthermore, the nitrite and the nitrate are not accumulated, and no N2O is generated, so that the composite microbial inoculum is safe and reliable.

Description

technical field [0001] The invention relates to a composite bacterial agent and a preparation method thereof. Background technique [0002] In recent years, with the development of my country's industry and the increase of agrochemicals, drinking water sources have been seriously polluted, and there is a development trend; slightly polluted source water refers to organic matter pollution, which makes the indicators of some projects exceed the hygienic standards. The pollutants contained in this kind of water are many kinds and complex in nature, and its water quality is mainly affected by the discharged industrial wastewater and domestic sewage. In the river water sources, the content of ammonia nitrogen, total phosphorus, chroma, organic matter, etc. exceeds the standard. On the water source of lakes and reservoirs, it is manifested as eutrophication of reservoirs and lakes, and in a certain period of time, algae will grow, causing water quality to deteriorate, and the sten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/11C12R1/01
Inventor 李伟光张多英王广智郜玉楠刘水
Owner HARBIN INST OF TECH
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