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Composite bacterium capable of quickly degrading 17Beta-estradiol and preparation method

A rapid degradation and estradiol technology, applied in the field of compound bacteria, can solve the problems of low adaptability and low degradation rate of 17β-estradiol, and achieve the effect of high synergistic symbiosis ability and efficient degradation

Inactive Publication Date: 2016-04-13
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low degradation rate of 17β-estradiol and low environmental adaptability of single degrading bacteria in the natural environment, and provide a compound bacteria capable of rapidly degrading 17β-estradiol

Method used

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  • Composite bacterium capable of quickly degrading 17Beta-estradiol and preparation method
  • Composite bacterium capable of quickly degrading 17Beta-estradiol and preparation method
  • Composite bacterium capable of quickly degrading 17Beta-estradiol and preparation method

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Embodiment 1

[0037] The invention can prepare the compound bacteria which can rapidly degrade 17β-estradiol.

[0038] 1. Domestication, cultivation and isolation of strains LM1 and LY1

[0039] Collect samples from pig farms, chicken farms, cattle farms and piled livestock and poultry manures and waste treatment facilities, mix them according to the same mass ratio, take 50g of the mixed sample into a 1000mL Erlenmeyer flask, and then add 500mL sterile water Shake at 150rpm for 20 minutes, let stand for 5 minutes, take the upper suspension, add 5% to 50mL of inorganic salt medium, then add 17β-estradiol with a final concentration of 20mg / L, and do Repeat 2 times. After shaking culture at 120rpm and 25℃ for 5 days, take 10ml of each and mix, then take 5ml of mixed culture and add it to 50ml of inorganic salt medium with 20mg / L of 17β-estradiol. Cultivate under the same conditions. After it becomes turbid, take 1ml of culture and add it to 50ml of 20mg / L of 17β-estradiol in inorganic salt medium...

Embodiment 2

[0050] The characteristic detection and application experiment of the compound bacteria capable of rapidly degrading 17β-estradiol of the present invention.

[0051] 1. The response law of environmental factors in the degradation of 17β-estradiol by compound bacteria

[0052] (1) The effect of carbon-nitrogen ratio on the degradation of 17β-estradiol by compound bacteria

[0053] Take 0.5mL of 17β-estradiol stock solution and add it to 12 250mL Erlenmeyer flasks containing 100mL of nitrogen-free inorganic salt medium (remove the ammonium sulfate in the original inorganic salt medium), and add different flasks to 4 Erlenmeyer flasks. The amount of ammonium sulfate makes the C:N ratios in the four Erlenmeyer flasks 1:40, 1:20, 1:10, 1:1, respectively. Take 2ml of the composite bacterial suspension and add it to the Erlenmeyer flask. Use the remaining In the Erlenmeyer flask, each carbon-nitrogen ratio treatment was repeated twice, and finally cultured at 28°C and 150r / min for 4 days, ...

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Abstract

The invention relates to a composite bacterium capable of quickly degrading 17Beta-estradiol. The composite bacterium can be used to quickly degrade steroid estrogen-17Beta-estradiol in livestock breeding waste, reducing environmental pollution and resourcefully using livestock excrement. The composite bacterium that can quickly degrade 17Beta-estradiol is composed of Acinetobacter calcoaceticus LM1 collected under CGMCC No. 10814 and Pseudomonas LY1 collected under CGMCC No. 10815. Mass experiments prove that Acinetobacter calcoaceticus LM1 and Pseudomonas LY1 are mixed according to a cell ratio of 1:1, giving optimal degrading efficiency for treating livestock breeding waste and 17Beta-estradiol in soil.

Description

Technical field [0001] The invention relates to a composite bacteria capable of rapidly degrading 17β-estradiol. The composite bacteria agent is used to rapidly degrade the steroid estrogen-17β-estradiol in livestock and poultry breeding wastes to reduce environmental pollution and realize livestock manure Resource utilization. Background technique [0002] The growth-promoting drugs used in large quantities in modern breeding and the hormones secreted by the animals themselves make the steroid estrogen content in the feces of livestock and poultry very high. For example, the average amount of 17β-estradiol eliminated through the feces of each cow reaches 165 micrograms / day In the form of urine, as high as 498 micrograms per day, the steroid estrogen emissions from livestock and poultry farms far exceed those of sewage treatment plants and become the main source of steroid estrogen pollution in the environment. The poisoning mechanism of steroid estrogen mainly includes simulati...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01C12R1/38A62D101/28
Inventor 李明堂林泳墨郝林琳田来明秦玉莹
Owner JILIN AGRICULTURAL UNIV
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