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Bacillus megaterium B16, inoculant and inoculant preparation method

A technology of Bacillus megaterium and B16, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of Bacillus megaterium application limitations, lack of nitrogenase activity and secretion of auxin

Active Publication Date: 2016-11-30
DONGGUAN BAODE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing Bacillus megaterium does not have nitrogenase activity and the ability to secrete auxin, which limits the application of Bacillus megaterium in agricultural production

Method used

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  • Bacillus megaterium B16, inoculant and inoculant preparation method
  • Bacillus megaterium B16, inoculant and inoculant preparation method
  • Bacillus megaterium B16, inoculant and inoculant preparation method

Examples

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Effect test

Embodiment 1

[0127] A type of Bacillus megaterium B16 in this embodiment, said Bacillus megaterium B16 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCCM 2015750. The Bacillus megaterium B16 has the DNA sequence of No. 1 in the sequence table. The nitrogenase activity of the Bacillus megaterium B16 was 698.367nmol / (mL·h), and the secretion amount of indole acetic acid produced during the growth and metabolism process was 400mg / L.

[0128] A method for preparing a bacterial agent of Bacillus megaterium B16, comprising the following steps:

[0129] (1) Separation and screening

[0130] Select soil samples containing nitrogen-fixing bacteria colonies of Bacillus megaterium B16, and obtain nitrogen-fixing bacteria colonies through isolation and screening medium culture;

[0131] (2) Purification and preservation

[0132] Purify the isolated and screened nitrogen-fixing bacteria colony on the purification preservation medium, culture and isolate a...

Embodiment 2

[0142] The difference between this embodiment and embodiment 1 lies in that the following steps are also included between the step (2) and step (3) of this embodiment:

[0143] (S1) Gram staining: Perform Gram staining on the purified single colony, and screen to obtain positive bacteria;

[0144] (S2) Spore staining: The positive bacteria were stained with spores, and a single colony of Gram-positive bacteria containing spores was screened.

[0145] A bacterial agent of Bacillus megaterium B16, which is prepared by the preparation method of a bacterial agent of Bacillus megaterium B16 described in this embodiment.

[0146] The rest of the content of this embodiment is the same as that of Embodiment 1, and will not be repeated here.

Embodiment 3

[0148] The difference between this example and Example 1 is that the nitrogenase activity of the Bacillus megaterium B16 in this example is 720.138nmol / (mL h), which produces the secretion of indole acetic acid in the process of growth and metabolism It was 420.53 mg / L.

[0149] The Bacillus megaterium B16 liquid inoculum of the present embodiment has a viable count of 400 million / g.

[0150] The rest of the content of this embodiment is the same as that of Embodiment 1, and will not be repeated here.

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Abstract

The invention relates to the technical field of microbial inoculants, in particular to a bacillus megaterium B16, an inoculant and an inoculant preparation method. The bacillus megaterium B16 is preserved in China Center for Type Collection with a preservation number of CCTCCM 2015750. The invention takes bacillus megaterium B16 as the starting strain to determine its nitrogenase activity and auxin secreting ability, and finds that the strain B16 has efficient nitrogenase activity and IAA secreting ability. The strain can supplement the nitrogen element needed in the growing process of paddy rice, corn and other graminaceous crops by free living nitrogen fixation, and also is a functional microbial strain capable of secreting indoleacetic acid to promote plant growth, thus having considerable economic value in the field of microbial fertilizer application. The bacillus megaterium B16 can be prepared into three B16 inoculants, i.e. liquid inoculant, powdery inoculant and particle inoculant, thereby providing convenience for putting it into agricultural production application.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a bacillus megaterium B16, an inoculum, and a preparation method for the inoculum. Background technique [0002] Agricultural production has long relied on chemical fertilizers, which not only wastes a lot of non-renewable resources, but also leads to environmental problems such as soil quality deterioration, agricultural product quality decline, and environmental pollution, which greatly affects human health and survival. [0003] Bacillus megaterium is a phosphorus-solubilizing, potassium-promoting and nitrogen-fixing bacterium, which can degrade phosphorus and potassium that cannot be used by plants in the soil, improve soil fertility, and fix nitrogen in the air to promote crop production. However, the existing Bacillus megaterium does not have nitrogenase activity and the ability to secrete auxin, which limits the application of Bacillus megaterium in agricultural...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C05F11/08C12R1/11C05G3/80
CPCC05F11/08C05G3/00C05G3/80C12N1/205C12R2001/11
Inventor 林敏杨国平孙旭生王亚君尹坤杨盼盼张学贤沈世华陈三凤谭志远燕永亮
Owner DONGGUAN BAODE BIOLOGICAL ENG
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