Bacillus megaterium B16, inoculant and inoculant preparation method
A technology of Bacillus megaterium and B16, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of Bacillus megaterium application limitations, lack of nitrogenase activity and secretion of auxin
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Embodiment 1
[0127] A type of Bacillus megaterium B16 in this embodiment, said Bacillus megaterium B16 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCCM 2015750. The Bacillus megaterium B16 has the DNA sequence of No. 1 in the sequence table. The nitrogenase activity of the Bacillus megaterium B16 was 698.367nmol / (mL·h), and the secretion amount of indole acetic acid produced during the growth and metabolism process was 400mg / L.
[0128] A method for preparing a bacterial agent of Bacillus megaterium B16, comprising the following steps:
[0129] (1) Separation and screening
[0130] Select soil samples containing nitrogen-fixing bacteria colonies of Bacillus megaterium B16, and obtain nitrogen-fixing bacteria colonies through isolation and screening medium culture;
[0131] (2) Purification and preservation
[0132] Purify the isolated and screened nitrogen-fixing bacteria colony on the purification preservation medium, culture and isolate a...
Embodiment 2
[0142] The difference between this embodiment and embodiment 1 lies in that the following steps are also included between the step (2) and step (3) of this embodiment:
[0143] (S1) Gram staining: Perform Gram staining on the purified single colony, and screen to obtain positive bacteria;
[0144] (S2) Spore staining: The positive bacteria were stained with spores, and a single colony of Gram-positive bacteria containing spores was screened.
[0145] A bacterial agent of Bacillus megaterium B16, which is prepared by the preparation method of a bacterial agent of Bacillus megaterium B16 described in this embodiment.
[0146] The rest of the content of this embodiment is the same as that of Embodiment 1, and will not be repeated here.
Embodiment 3
[0148] The difference between this example and Example 1 is that the nitrogenase activity of the Bacillus megaterium B16 in this example is 720.138nmol / (mL h), which produces the secretion of indole acetic acid in the process of growth and metabolism It was 420.53 mg / L.
[0149] The Bacillus megaterium B16 liquid inoculum of the present embodiment has a viable count of 400 million / g.
[0150] The rest of the content of this embodiment is the same as that of Embodiment 1, and will not be repeated here.
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