Method for measuring nitrogenase activity of azotobacteria in sugarcane
A determination method and technology of nitrogenase, applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the problems of inaccurately reflecting the activity of nitrogenase, and achieve low cost, simple composition, and easy preparation Effect
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[0091] 1. Composition and preparation of SH medium
[0092] Weigh 100g of sucrose, add distilled water to make the volume 850ml, adjust the pH to 5.8 with 1N hydrochloric acid, adjust the volume to 1000ml, divide it, and heat it at 121°C for 15min for later use.
[0093] 2). Sampling and sample processing
[0094] Take the cane stems of ROC25 perennial root cane +5 to +7 leaves back to the room, rinse with tap water, distilled water, sterile water, dewater with sterile paper, remove the cane skin, take the middle part of the +6 leaf cane stems and chop them finely and evenly. Weigh the sample and repeat it three times. The weight of the sample is 2.0g, 1.9g, and 2.1g, respectively, and transfer it to a 60ml culture bottle, add culture medium to a total volume of 10ml, and seal it with a rubber stopper;
[0095] 3). Incubation
[0096] Transfer the sealed culture bottle to a shaker with a temperature of 28°C and a rotation speed of 60-70 rpm. After culturing for one day, inje...
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