Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12

Abstract

The invention relates to the field of microorganisms and particularly relates to a nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and an application of the nitrogen-fixing bacterium PJ 12, and the conservation number is CGMCC No.14659. The activity analysis of nitrogenase proves that a provided bacterial strain has a stronger activity of nitrogenase, and the activity of the dinitrogenase is higher under the condition of 5-21% oxygen concentration. The activity of the dinitrogenase is still kept at the petroleum concentration of 1%-5%. More than that, the provided bacterial strain further has the capability of degrading long-chain alkane, the degrading capability is up to 37-100%, and the provided bacterial strain has an excellent positive application prospect on balancing nitrogen and degrading petroleum pollutants in the remediation of petroleum polluted soil in the future.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a nitrogen-fixing bacterium strain capable of degrading petroleum and tolerant to petroleum. Background technique [0002] In bioremediation, microorganisms play an important role. In the process of natural evolution, microorganisms metabolize organic substances through different catalytic mechanisms, and "store" functional enzymes as raw materials for evolution, which can coexist with various types of organic components, and are particularly good at using natural or synthetic organic substrates as carbon Since the 1940s, the use of microorganisms to control oil pollution has been considered a powerful strategy. [0003] During the biodegradation process, the addition of nutrients plays an important role in activating indigenous microorganisms, promoting microbial metabolism and degrading petroleum hydrocarbons in the environment. However, excessive use of nutrients can lead to some pot...

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Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil

Aerobic biodegradation of petroleum is often limited by combined nitrogen levels

In 2011, Mercer summarized the reported effects of biological treatment (bio-stimulation and degradative agent addition) in beach pollution treatment, and found that 10 reports were successful and 6 reports failed

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