Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12

A technology of PJ12 and nitrogen-fixing bacteria, applied in the field of microorganisms, can solve problems such as excessive consumption of oxygen, reduction of oil degradation ability of indigenous microorganisms, limitations of aerobic biological oil degradation, etc., and achieve positive application prospects

Active Publication Date: 2018-08-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil
Aerobic biodegradation o

Method used

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  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12
  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12
  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12

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Embodiment 1

[0038] The nitrogenase activity analysis of bacterial strain PJ 12 described in embodiment 1 present invention

[0039] 1. Nitrogenase determination conditions:

[0040] 1) The strain PJ 12 of the present invention (referred to as PJ 12 strain) was activated twice in GMCY medium at 30°C, and after being transferred to liquid culture for 10 hours, the bacterial cells were washed with K.oxytoca (nitrogen-free) medium, and the initial OD was adjusted. 600 =0.2 or so, inoculate into 5mL K.oxytoca (nitrogen-free) medium anaerobic tube, 30°C, rotation speed 200rmp.

[0041] 2) After culturing for 4hr, 8hr, 12hr, 16hr, 20hr, 24hr, 28hr, and 32hr, replace the anaerobic tube with argon, adjust the different oxygen concentrations, inject acetylene, and inject trichloroacetic acid to stop after 0.5h of reaction After the reaction, 100 μL of gas was taken to measure the ethylene content in the gas by gas spectrometer (SQ-204 gas chromatograph, FID detector, 2 m packed column, GDX502 insi...

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Abstract

The invention relates to the field of microorganisms and particularly relates to a nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and an application of the nitrogen-fixing bacterium PJ 12, and the conservation number is CGMCC No.14659. The activity analysis of nitrogenase proves that a provided bacterial strain has a stronger activity of nitrogenase, and the activity of the dinitrogenase is higher under the condition of 5-21% oxygen concentration. The activity of the dinitrogenase is still kept at the petroleum concentration of 1%-5%. More than that, the provided bacterial strain further has the capability of degrading long-chain alkane, the degrading capability is up to 37-100%, and the provided bacterial strain has an excellent positive application prospect on balancing nitrogen and degrading petroleum pollutants in the remediation of petroleum polluted soil in the future.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a nitrogen-fixing bacterium strain capable of degrading petroleum and tolerant to petroleum. Background technique [0002] In bioremediation, microorganisms play an important role. In the process of natural evolution, microorganisms metabolize organic substances through different catalytic mechanisms, and "store" functional enzymes as raw materials for evolution, which can coexist with various types of organic components, and are particularly good at using natural or synthetic organic substrates as carbon Since the 1940s, the use of microorganisms to control oil pollution has been considered a powerful strategy. [0003] During the biodegradation process, the addition of nutrients plays an important role in activating indigenous microorganisms, promoting microbial metabolism and degrading petroleum hydrocarbons in the environment. However, excessive use of nutrients can lead to some pot...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C05F11/08C12R1/065C02F101/16
CPCC02F3/34C02F2101/16C05F11/08C12N1/205C12R2001/065
Inventor 王磊陈琳陈宏坤张心昱郑国砥
Owner CHINA AGRI UNIV
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