Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12

A technology of PJ12 and nitrogen-fixing bacteria, applied in the field of microorganisms, can solve problems such as excessive consumption of oxygen, reduction of oil degradation ability of indigenous microorganisms, limitations of aerobic biological oil degradation, etc., and achieve positive application prospects

Active Publication Date: 2018-08-21
CHINA AGRI UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil
Aerobic biodegradation o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12
  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12
  • Nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 12

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The nitrogenase activity analysis of bacterial strain PJ 12 described in embodiment 1 present invention

[0039] 1. Nitrogenase determination conditions:

[0040] 1) The strain PJ 12 of the present invention (referred to as PJ 12 strain) was activated twice in GMCY medium at 30°C, and after being transferred to liquid culture for 10 hours, the bacterial cells were washed with K.oxytoca (nitrogen-free) medium, and the initial OD was adjusted. 600 =0.2 or so, inoculate into 5mL K.oxytoca (nitrogen-free) medium anaerobic tube, 30°C, rotation speed 200rmp.

[0041] 2) After culturing for 4hr, 8hr, 12hr, 16hr, 20hr, 24hr, 28hr, and 32hr, replace the anaerobic tube with argon, adjust the different oxygen concentrations, inject acetylene, and inject trichloroacetic acid to stop after 0.5h of reaction After the reaction, 100 μL of gas was taken to measure the ethylene content in the gas by gas spectrometer (SQ-204 gas chromatograph, FID detector, 2 m packed column, GDX502 insi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of microorganisms and particularly relates to a nitrogen-fixing bacterium PJ 12 capable of degrading petroleum and tolerating petroleum and an application of the nitrogen-fixing bacterium PJ 12, and the conservation number is CGMCC No.14659. The activity analysis of nitrogenase proves that a provided bacterial strain has a stronger activity of nitrogenase, and the activity of the dinitrogenase is higher under the condition of 5-21% oxygen concentration. The activity of the dinitrogenase is still kept at the petroleum concentration of 1%-5%. More than that, the provided bacterial strain further has the capability of degrading long-chain alkane, the degrading capability is up to 37-100%, and the provided bacterial strain has an excellent positive application prospect on balancing nitrogen and degrading petroleum pollutants in the remediation of petroleum polluted soil in the future.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a nitrogen-fixing bacterium strain capable of degrading petroleum and tolerant to petroleum. Background technique [0002] In bioremediation, microorganisms play an important role. In the process of natural evolution, microorganisms metabolize organic substances through different catalytic mechanisms, and "store" functional enzymes as raw materials for evolution, which can coexist with various types of organic components, and are particularly good at using natural or synthetic organic substrates as carbon Since the 1940s, the use of microorganisms to control oil pollution has been considered a powerful strategy. [0003] During the biodegradation process, the addition of nutrients plays an important role in activating indigenous microorganisms, promoting microbial metabolism and degrading petroleum hydrocarbons in the environment. However, excessive use of nutrients can lead to some pot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34C05F11/08C12R1/065C02F101/16
CPCC02F3/34C02F2101/16C05F11/08C12N1/205C12R2001/065
Inventor 王磊陈琳陈宏坤张心昱郑国砥
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap