Rhizobium for promoting growth of vicia sativa L. and application of rhizobium

A technology of pea rhizobia and arrowhead pea is applied in the field of agricultural microorganisms, which can solve the problems of consumption of soil nitrogen and decline of soil fertility.

Active Publication Date: 2017-07-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

If the planted legumes do not have corresponding high-efficiency rhizobia strains to symbiotically form nodules and fix nitrogen in the air, they will completely depend on the combined nitrogen in the soil, and instead of supplementing it, they will consume nitrogen in the soil, resulting in soil Decreased fertility

Method used

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  • Rhizobium for promoting growth of vicia sativa L. and application of rhizobium
  • Rhizobium for promoting growth of vicia sativa L. and application of rhizobium
  • Rhizobium for promoting growth of vicia sativa L. and application of rhizobium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, Isolation and Identification of Pea Rhizobium (Rhizobium leguminosarum) JW1401 CGMCC11876

[0029] 1. Isolation of strains

[0030] From Huzhu Tu Autonomous County and Ping'an District, Haidong City, Qinghai Province, wild arrowhead pea root nodule samples were collected, and on a flat plate (get mannitol 10g, yeast extract powder 1g, dipotassium hydrogen phosphate 0.5g, calcium sulfate 0.2g, magnesium sulfate 0.2g, 0.1g of sodium chloride, 1ml of ammonium molybdate aqueous solution with a mass content of 1% and 1ml of boric acid aqueous solution with a mass content of 1%, 1ml of 0.5% Congo red, 20g of agar, dilute to 1L with water, and the pH value is 6.8-7.0) Strain JW1401 was isolated by streaking.

[0031] 2. Identification of strains

[0032] 2.1. Morphological identification

[0033] The strain JW1401, which is in the logarithmic growth phase and has a stable colony size, and is isolated and purified in the above step 1, is described for the state of...

Embodiment 2

[0040] Embodiment 2, the preparation of pea rhizobia JW1401 bacterial agent

[0041] 1. Slope culture of pea rhizobia JW1401

[0042] Rhizobia pea JW1401 of Example 1 was picked and inoculated on a solid medium for slant culture, and cultured at 28° C. for 56 hours to obtain Rhizobia pea JW1401 cultured on a slant.

[0043] 2. Activation of bacteria

[0044] Pick the Rhizobia pea JW1401 cultured on the slant in step 1, inoculate it in 500 mL of liquid medium, and culture it at 28° C. for 48 hours to obtain the Rhizobia pea JW1401 bacterial liquid.

[0045] 3. Seed jar cultivation

[0046] Take 3L of the pea rhizobium JW1401 bacterial liquid in step 2, insert it into a 100L seed tank (containing 60L liquid medium) for seed culture, and cultivate it by shaking at 30°C and 120rpm for 56 hours to obtain the pea rhizobium JW1401 seed liquid .

[0047] 4. Fermentation tank culture

[0048] Put the pea rhizobium JW1401 seed liquid in step 3 into a 1000L fermenter (containing 600...

Embodiment 3

[0112] Embodiment 3, the hydroponic test of pea rhizobia JW1401 bacterial agent inoculation arrowhead pea

[0113] Arrowhead pea adopts the filter paper bridge test tube hydroponics method. Filter paper is cut into strips, and its width is 1.6cm respectively, and is made into M type, and the central recess is made into V-shaped aperture according to the size of seedlings, and the height of M type filter paper is the length of test tube minus 4cm. The test tube used is 2.0cm×20cm.

[0114] Put the prepared nitrogen-free nutrient solution into test tubes with filter paper supports, the height of which is located in the recess of the M-shaped filter paper, repeat 4 test tubes for each treatment, and set up 4 control tubes, after filling, use durable After the high-temperature and high-pressure plastic film is sealed, it is sterilized at 121°C for 1 hour for later use.

[0115] Select the Qinghai local arrowhead pea variety Qinghai Huijian seeds with uniform size, soak them in 9...

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Abstract

The invention discloses a rhizobium for promoting growth of vicia sativa L. and an application thereof. The rhizobium is a rhizobium leguminosarum, has a strain number of JW1401, and has a preservation number of CGMCCNo.11876 in the China General Microbiological Culture Collection Center. A test proves that the inoculation combination of the rhizobium leguminosarumJW1401CGMCC 11876 and the vicia sativa L. has a significant effect of increasing the production of the vicia sativa L., has relatively high practicability and generalizability, and is expected to become a novel excellent combination technology in the field of application of the rhizobium for the vicia sativa L.. The rhizobium provided by the invention has a broad application prospect in the planting industry of the vicia sativa L..

Description

technical field [0001] The invention relates to a rhizobia strain promoting the growth of arrowhead pea in the field of agricultural microorganisms and its application. Background technique [0002] Arrowhead pea (Vicia sativa L.) is an annual or perennial herbaceous leguminous plant. Arrowhead pea is suitable for growing on sandy soil and clay soil with dry climate, cold tolerance and cool temperature, and good drainage. The suitable soil pH value is 6.5-8.5, and it has a wide range of adaptability. Slightly less stress resistance than sweet potato. Early start, fast growth, early maturity, high and stable seed yield, fresh matter nutrient content is N 0.64%, P 2 o 5 0.1%, K 0.59%. Arrowhead pea is green manure and good pasture. It is distributed wildly in the grasslands and mountains of Jiangsu, Jiangxi, Taiwan, Shaanxi, Yunnan, Qinghai, Gansu and other provinces (regions) of our country. It is born in barren hills, fieldside grasses and forests at an altitude of 50-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00C05F11/08C12R1/41
CPCA01N63/00A01N63/10C05F11/08C12N1/20C12N1/205C12R2001/41
Inventor 韩梅马晓彤曹卫东张宏亮王雪翠
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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