Nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and application thereof

A Stenotrophomonas and maltophilia technology, which is applied to nitrogen-fixing maltophilia Stenotrophomonas C4Y4 and its application field, and can solve the problems of soil structure damage, soil compaction, soil fertility reduction and the like

Inactive Publication Date: 2010-07-21
广西绿满地农资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to overharvesting, full reclamation and unreasonable fertilization can also lead to the decline of eucalyptus plantation
(2) Moisture problem
[0004] Eucalyptus is a non-legume plant that does not have the ability to fix nitrogen and self-fertilize. At present, the fast growth and high yield of eucalyptus plantation complete

Method used

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  • Nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and application thereof
  • Nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and application thereof
  • Nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Isolation and purification of nitrogen-fixing Stenotrophomonas maltophilia strain C4Y41

[0024] The fine roots with nodules were collected from the lateral roots of Podocarpus lobata, rinsed with tap water, sterilized with 75% ethanol for 1 min, and then sterilized with 0.1% mercuric chloride for 5-6 min. Grind the root nodules and fine roots in a sterilized mortar, add a small amount of sterile water, grind well, cover and let stand, and clarify for a while. Take the supernatant and inoculate it on the YMA medium that has been added with Congo red by streaking method, culture at 28°C for about 2 days, select the colonies that do not absorb Congo red, repeat the streaking and screening culture, and then select round, milky white, and producing colonies. Mucoid, neatly bordered colonies. Microscopically, there are flagella, motile, and no spores. Obtain purified strains. The composition and content of the YMA medium are: 0.5g dipotassium hydrogen phosphate, 0.5g m...

Embodiment 2

[0030] Effects of inoculating nitrogen-fixing Stenotrophomonas maltophilia C4Y41 on eucalyptus seedlings by soaking roots

[0031] This experiment was carried out in the nursery of Guangxi State-run Gaofeng Forest Farm located in the suburbs of Nanning.

[0032] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.

[0033] Root soaking method inoculation: before transplanting the above-treated seedlings into the nutrition cup, soak the roots of the seedlings with Stenotrophomonas maltophilia C4Y41 bacteria solution for 20-30 minutes, and then plant them in the nutrition cup. The soil is ye...

Embodiment 3

[0039] Effects of Spray Inoculation of Nitrogen-fixing Stenotrophomonas maltophilia C4Y41 on Eucalyptus Seedlings

[0040] This experiment was carried out in the nursery of Nanning Vegetable Research Institute in the suburb of Nanning.

[0041] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.

[0042] Spray inoculation method: After the seedlings are transplanted into the nutrition cup, use a watering can to evenly spray the Stenotrophomonas maltophilia C4Y41 bacteria solution on the eucalyptus seedlings planted in the nutrition cup, inoculate a total of 20,000 seedlings, and do not wat...

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Abstract

The invention relates to a nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and an application thereof. The strain is stored in the common microorganism center of China microorganism strain management committee and has a storage number of CGMCC No.3349. The strain is a new nitrogen fixation strain obtained by applying a general microorganism-separating and purifying method on podocarpus root nodules, has the nitrogen fixation enzyme activity of 0.019-0.030nmol*g-1*h-1 and enables the height of inoculated eucalyptus seedlings to increase by 2.20-22.12 percent, the ground diameter to increase by 22.15-36.84 percent, the average biomass to increase by 40.47-60.81 percent, the chlorophyll content to increase by 12.68-34.07 percent and the nitrate reductase activity to increase by 21.82-36.69 percent. The strain can be applied as a biological fertilizer for stimulating the growth of the eucalyptus seedlings.

Description

technical field [0001] The patent of the invention belongs to the field of biological technology, and relates to a nitrogen-fixing maltophilic stenotrophomonas strain C4Y4 and its application. Background technique [0002] At present, there are about 50 million hm in the world 2 of tropical plantations, and an annual increase of 3 million hm 2 left and right (Binkley et al., 2004). Among them, eucalyptus (Eucalyptus sp.) accounts for about 40% of the total tropical plantation area, and accounts for more than 50% of the timber output, and is playing an increasingly important role. The area of ​​eucalyptus plantations in China has reached 1.7 million hectares 2 Eucalyptus has become one of the three major afforestation tree species in my country, and its afforestation area is second only to India and Brazil, ranking third in the world. Eucalyptus is fast-growing, high-yielding, high-quality, and the average annual growth of the optimal hybrid family can reach 69.8m 3 / hm ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 吕成群黄宝灵
Owner 广西绿满地农资有限公司
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