High-glyphosate-tolerance EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase), and coding gene and application thereof

An EPSP synthase and gene technology, which is applied in the field of biology, can solve the problems of decreased glyphosate inhibition constant, decreased binding ability, decreased PEP affinity, etc.
CN103484438AActive Publication Date: 2014-01-01INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2014-01-01

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Abstract

The invention provides a novel 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase) and a coding gene thereof. The EPSP synthase is (1) a protein composed of amino acid sequence disclosed as SEQ ID NO.2, or (2) a (1)-derived protein with glyphosate tolerance and EPSP synthase activity obtained by substitution, deletion or addition of one or more amino acids on the basis of the amino acid sequence disclosed as SEQ ID NO.2. The invention also provides a preparation method of the EPSP synthase, and application of the EPSP synthase and coding gene thereof. After the EPSP synthase coding gene is transplanted and expressed into a plant, the obtained transgenic plant has higher tolerance to glyphosate.
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Description

technical field

[0001] The invention belongs to the field of biology, and relates to a microbial-sourced 5-enolpyruvylshikimate-3-phosphate synthase (5-enolpyruvylshikimate-3-phosphate Synthase, EPSP synthase), its coding gene and its ability to cultivate resistant Application of glyphosate in plants. Background technique

[0002] Glyphosate was first discovered and synthesized by John E Franz of Monsanto in the early 1970s. Glyphosate has excellent characteristics such as high efficiency, low toxicity, fast degradation in the environment, and broad-spectrum killing, and the preparation process is simple. Due to the outstanding characteristics of glyphosate, it has rapidly developed into a herbicide with the largest production and sales volume and the most widely used area in agricultural production in the past few decades.

[0003] The mechanism of action of glyphosate is mainly to competitively inhibit the EPSPS that catalyzes PEP and S3P to generate EPSP in the shikimat...

Claims

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