Axmi-031, axmi-039, axmi-040 and axmi-049, a family of novel delta-endotoxin genes and methods for their use
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
a deltaendotoxin and gene family technology, applied in the field of molecular biology, can solve problems such as larval death
Active Publication Date: 2009-07-16
BASF AGRICULTURAL SOLUTIONS SEED LLC
View PDF4 Cites 340 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Benefits of technology
[0014]The compositions and methods of the invention are useful for the production of organisms with pesticide resistance, specifically bacteria and plants. These organisms and compositions derived from them are desirable for agricultural purposes. The compositions of the invention are also useful for generating altered or improved delta-endotoxin proteins that have pesticidal activity, or for detecting the presence of delta-endotoxin proteins or nucleic acids in products or organisms.
Problems solved by technology
This toxin binds to apical brush border receptors in the midgut of the target larvae and inserts into the apical membrane creating ion channels or pores, resulting in larval death.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
example 1
Growth of ATX9387 and Preparation of Extracts
[0102]Strain ATX9387, identified as a member of the Bacillus cereus / Bacillus thuringiensis group by MIDI analysis, was grown in T3 medium at 30 degrees for times ranging from 16 hours to 5 days. Cultures were centrifuged and the supernatants were passed through 0.2 micron filters, resulting in sterile supernatants
example 2
C. elegans Bioassay
[0103]Caenorhabitis elegans (“C. elegans”) hermaphrodites were reared as known in the art, to generate populations of healthy animals for bioassay. General procedures for growth, harvesting, and genetic manipulation of C. elegans including growth media, etc. may be found in the art, for example, in Wood, ed. (1988) The Nematode Caenorhabditis elegans (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).
[0104]Sterile supernatants from strain ATX9387 were tested for activity on C. elegans. Bioassays were performed in 96-well plates. Five to ten nematodes were added to 80 μl of S medium (Wood, supra) and were mixed with 20 μl of sterile supernatant, 0.5 μl of concentrated HB101 (prepared as described in Wood, supra) and rifampicin (final concentration of 0.1 μg / l). Assays were allowed to proceed at room temperature for 3 days and nematodes were quantitated. Negative control samples (T3 medium or sterile supernatants from inactive strains) contained hundred...
example 3
Activity of ATX9387 on Soybean Cyst Nematodes
[0105]A sterile supernatant of a 5-day culture of ATX9387 was concentrated 40-fold and fed to SCN J2 nematodes. Nematodes feeding on sterile supernatant were reproducibly observed to be sluggish, and exhibited higher motility than nematodes fed extract of a negative control concentrated to the same extent.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
temperature
aaaaa
aaaaa
temperature
aaaaa
aaaaa
pH
aaaaa
aaaaa
Login to view more
Abstract
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
Description
CROSS REFERENCE TO RELATED APPLICATION[0001]This application is a divisional of U.S. application Ser. No. 11 / 762,886, filed Jun. 14, 2007, which claims the benefit of U.S. Provisional Application Ser. No. 60 / 813,774, filed Jun. 14, 2006, the contents of which are herein incorporated by reference in their entirety.REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY[0002]The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named “366899_SequenceListing.txt”, created on Jan. 31, 2009, and having a size of 347 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety.FIELD OF THE INVENTION[0003]This invention relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These proteins and the nucleic acid sequences tha...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(United States)
Login to view more