Kit for testing tiny gene loss of male Y chromosome, and testing method

A Y chromosome and gene detection technology, applied in the field of male Y chromosome microdeletion gene detection kits, can solve the problems of cumbersome methods, difficult to popularize, lack of standard screening programs, etc., and achieve the effect of stable results and low cost

What is AI technical title?
AI technical title is built by PatSnap AI team. It summarizes the technical point description of the patent document.
A Y chromosome and gene detection technology, applied in the field of male Y chromosome microdeletion gene detection kits, can solve the problems of cumbersome methods, difficult to popularize, lack of standard screening programs, etc., and achieve the effect of stable results and low cost

CN101092648AInactive Publication Date: 2007-12-26亚能生物技术(深圳)有限公司

1 Cites 16 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.

Viewing Examples

Smart Image

Examples

Experimental program

Comparison scheme

Effect test

Embodiment 1

[0052] 1. Materials

[0053] 1. Instruments: PCR instrument, centrifuge

[0054] 2. Primer design: the present invention has designed 15 pairs of PCR primers to specifically amplify the AZFSTSs sequence respectively, and simultaneously designs 1 pair of internal control PCR primers to amplify the male-specific Sry (sex-determining region of Y-chromesome) gene to indicate PCR proceeded normally.

[0055] Table 1 Primer list

[0056] Primer name

serial number

Amplified object

sY254-F

sY254-R

SEQ ID NO: 1

SEQ ID NO: 2

Specific amplification of Y chromosome AZFc

Subregion sY254 sequence

Sy143-F

sY143-R

SEQ ID NO: 3

SEQ ID NO: 4

Specific amplification of Y chromosome AZFb

Subregion sY143 sequence

sY242-F

sY242-R

SEQ ID NO: 5

SEQ ID NO: 6

Specific amplification of Y chromosome AZFc

Subregion sY242 sequence

sY255-F

sY255-R

SEQ ID NO: 7

SEQ ID NO: 8

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

PUM

Login to View More

Abstract

This invention relates to test kit and method for detecting male Y chromosome microdeletion genes. The test kit contains 30 primers for amplifying AZF of Y chromosome, and 2 primers for amplifying male-specific Sry gene. The test kit can be used for detecting male Y chromosome microdeletion genes, and has such advantages as high efficiency, low cost and stable result.

Description

technical field [0001] The invention relates to the field of gene deletion detection, in particular to a male Y chromosome microdeletion gene detection kit and detection method. Background technique [0002] About 15% of couples in the world are plagued by infertility, and male factors account for about half of them. After excluding vas deferens obstruction, Klinefelter's disease, and other genitourinary causes, the most likely cause of severe oligospermia and azoospermia is abnormal spermatogenesis genes. With regard to spermatogenesis genes, particular attention was paid to the 11 interval on the long arm of the Y chromosome. The Y chromosome constitutes 2% of the human genome and consists of a short arm (Yp) and a long arm (Yq). Cytogenetic and molecular biological studies of azoospermia and severe oligospermia have also confirmed the existence of azoospermia factor (AZF) on the Y chromosome, which has four clear, non-overlapping subregions, namely AZFa, AZFb, AZFc and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

Application Information

Patent Timeline

26 Dec 2007

Publication

CN101092648A

IPC: C12Q1/68; C12N15/11

Inventors: TIAN JIE; REN WEI