Kit for testing tiny gene loss of male Y chromosome, and testing method

A Y chromosome and gene detection technology, applied in the field of male Y chromosome microdeletion gene detection kits, can solve the problems of cumbersome methods, difficult to popularize, lack of standard screening programs, etc., and achieve the effect of stable results and low cost

Inactive Publication Date: 2007-12-26
亚能生物技术(深圳)有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Multiplex PCR electrophoresis: Multiplex PCR is currently the most widely used method for the detection of Y chromosome microdeletions. It is simple, fast, sensitive, specific, economical and high-throughput. It is an ideal method for detecting AZF. Compared with higher technical requirements and lack of standard screening protocol, the results of each laboratory are not the same
[0005] 2. Probe technology: Southern blot or Northern blot with specific probes is an effective method to study the relationship between AZF microdeletion and male infertility, but the method is too cumbersome and difficult to promote, an

Method used

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  • Kit for testing tiny gene loss of male Y chromosome, and testing method
  • Kit for testing tiny gene loss of male Y chromosome, and testing method
  • Kit for testing tiny gene loss of male Y chromosome, and testing method

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Embodiment 1

[0052] 1. Materials

[0053] 1. Instruments: PCR instrument, centrifuge

[0054] 2. Primer design: the present invention has designed 15 pairs of PCR primers to specifically amplify the AZFSTSs sequence respectively, and simultaneously designs 1 pair of internal control PCR primers to amplify the male-specific Sry (sex-determining region of Y-chromesome) gene to indicate PCR proceeded normally.

[0055] Table 1 Primer list

[0056] Primer name

serial number

Amplified object

sY254-F

sY254-R

SEQ ID NO: 1

SEQ ID NO: 2

Specific amplification of Y chromosome AZFc

Subregion sY254 sequence

Sy143-F

sY143-R

SEQ ID NO: 3

SEQ ID NO: 4

Specific amplification of Y chromosome AZFb

Subregion sY143 sequence

sY242-F

sY242-R

SEQ ID NO: 5

SEQ ID NO: 6

Specific amplification of Y chromosome AZFc

Subregion sY242 sequence

sY255-F

sY255-R

SEQ ID NO: 7

SEQ ID NO: 8

...

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Abstract

This invention relates to test kit and method for detecting male Y chromosome microdeletion genes. The test kit contains 30 primers for amplifying AZF of Y chromosome, and 2 primers for amplifying male-specific Sry gene. The test kit can be used for detecting male Y chromosome microdeletion genes, and has such advantages as high efficiency, low cost and stable result.

Description

technical field [0001] The invention relates to the field of gene deletion detection, in particular to a male Y chromosome microdeletion gene detection kit and detection method. Background technique [0002] About 15% of couples in the world are plagued by infertility, and male factors account for about half of them. After excluding vas deferens obstruction, Klinefelter's disease, and other genitourinary causes, the most likely cause of severe oligospermia and azoospermia is abnormal spermatogenesis genes. With regard to spermatogenesis genes, particular attention was paid to the 11 interval on the long arm of the Y chromosome. The Y chromosome constitutes 2% of the human genome and consists of a short arm (Yp) and a long arm (Yq). Cytogenetic and molecular biological studies of azoospermia and severe oligospermia have also confirmed the existence of azoospermia factor (AZF) on the Y chromosome, which has four clear, non-overlapping subregions, namely AZFa, AZFb, AZFc and...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor TIAN JIEREN WEIXIANG ZHULIAO SHENGYUNZOU YAODONG
Owner 亚能生物技术(深圳)有限公司
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