Method for breeding zebra fish with wnt16 gene deletion through gene knockout

Abstract

The invention discloses a method for breeding zebra fish with wnt16 gene deletion through gene knockout. Compared with the prior art, the method has the advantages that specific genes can be more efficiently and more accurately silenced in a genome of a living body, in addition, the preparation is simple, the cost is low, moreover, a plurality of sites on a target gene can be simultaneously shorn, and single genes of any number can be silenced. The method is used for carrying out the relevant researches on gene and skeletal development, also can be used for carrying out the exploration research of other aspects, is used for detecting that whether the deletion of the wnt16 gene has relevance with the development of other organs, such as the heart, or not, and has a very good medical research value, meanwhile, the zebra fish with the wnt16 gene knocked out has the obviously shortened growth cycle, and thus the commercial value is good.

Description

technical field [0001] The invention relates to the technical field of gene knockout, in particular to a method for gene knockout breeding wnt16 gene-deficient zebrafish. Background technique [0002] The Wnt gene is located at human 12q13 and encodes a transcription factor of 356 amino acids. It is generally believed that the Wnt signal transduction pathway is divided into canonical Wnt signaling pathway and non-canonical Wnt signaling pathway. The canonical Wnt signaling pathway is also known as the Wnt / p-catenin signaling pathway. The molecular mechanism of Wnt / p-catenin signal transduction is highly conserved in different species. Wnt16 is a non-canonical wnt ligand. It is generally believed that this gene mediates the β-catenin signaling pathway, can negatively regulate RANK signaling, inhibit the formation of osteoclasts, and participate in processes such as bone formation and bone resorption. Through gene differential expression profile analysis and genome associat...

AI Technical Summary

Problems solved by technology

Traditional gene targeting technology is based on embryonic stem cells (ESC) and homologous recombination technology, so the efficiency of targeting technology is extremely low

At the beginning of 2013, a new artificial endonuclease clustered regularly interspacedshortpalindromic repeats (CRISPR) / CRISPR-associated (Cas) 9 can more efficiently and accurately silence specific genes in the genome of organisms, and is simple and low-cost , and can cut multiple sites on the target gene at the same time, and silence any number of single genes, but at the same time, this technology has certain defects, and its off-target rate is relatively high

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