Preparation method for antibacterial coat for fixing various cell growth factors on medical metal

A growth factor, metal surface technology, applied in coating, medical science, prosthesis, etc., can solve the problems of small amount of growth factor, interact, reduce efficacy, etc., achieve large specific surface area, large fixed amount, osteoinductive strong effect

Inactive Publication Date: 2013-07-10
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the existing multi-factor modification assembles growth factors layer by layer through polymer films, the existing problems are that the assembly efficiency of polymer films is low, the amount of fixed growth factors is small,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for preparing an antibacterial coating of multiple cell growth factors fixed on a medical metal surface, the specific steps of which are as follows:

[0033] A. A variety of cell growth factors were encapsulated into polysaccharide nanoparticles:

[0034] A1, bone morphogenetic protein-2, vascular endothelial growth factor, transforming growth factor β 2 Dissolve in heparin (polyanion) solution with a concentration of 2 mg / ml respectively, and correspondingly obtain bone morphogenetic protein-2, vascular endothelial growth factor, transforming growth factor-β 2 Three groups of heparin solutions, including bone morphogenetic protein-2, vascular endothelial growth factor, transforming growth factor β 2 The concentration is 0.5μg / ml;

[0035] A2, the preparation of step A1 containing bone morphogenetic protein-2, vascular endothelial growth factor, transforming growth factor β 2 The heparin solution was mixed with 0.5 mg / ml chitosan (polycation) solution at a v...

Embodiment 2

[0051] The operation of this example is basically the same as that of Example 1, except that the antibiotic used in Example 1 is changed from gentamycin to neomycin.

Embodiment 3

[0053] The operation of this example is basically the same as in Example 1, except that the antibiotic used in Example 1 is changed from gentamicin to cephradine.

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PUM

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Abstract

The invention discloses a preparation method for an antibacterial coat for slowly releasing various cell growth factors on medical metal. According to the method, antibiotic with amino is grafted on graphene oxide by covalent binding, the graphene oxide grafted with the antibiotic is taken as a carrier, and nano-particles, which wrap the various cell growth factors respectively, are fixed between grapheme oxide layers by layer-by-layer self-assembly, so that the coat, which carries the various cell growth factors and the antibiotic at the same time, is obtained on the surface of the medical metal. The coat prepared by the method can independently control the various cell growth factors to be slowly and orderly released, the fixation amount of the cell growth factors is large and the bone induction effect is strong. In addition, the coat can release the antibiotic in the whole process of the slow release of the cell growth factors, thus achieving a long-term antibacterial effect. In addition, the cell growth factors do not interact with the antibiotics, so that the respective efficacy of the cell growth factors and the antibiotics is given full play.

Description

technical field [0001] The invention relates to a method for preparing an antibacterial coating for fixing multiple cell growth factors on a medical metal surface. Background technique [0002] New bone regeneration on the surface of biomedical metals such as titanium and titanium alloys is slow, and loading cell growth factors on the surface can greatly improve the bone repair ability of the surface. In the process of bone tissue repair, a variety of growth factors need to act synergistically to promote new bone growth. Therefore, it is often necessary to modify medical metals with various growth factors. When the existing multi-factor modification assembles growth factors layer by layer through polymer films, the existing problems are that the assembly efficiency of polymer films is low, the amount of fixed growth factors is small, and the promotion effect on bone growth needs to be improved. At the same time, bacterial infection is prone to occur during the implantation...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/34A61L27/54
Inventor 鲁雄韩璐王振铭姜丽丽郭亚楠孙洪龙
Owner SOUTHWEST JIAOTONG UNIV
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