Phenyloboricacid-modified cationic polymer and composite method and application thereof

A technology of cationic polymers and phenylboronic acid, which is applied in the direction of introducing foreign genetic material using carriers and recombinant DNA technology, etc., can solve the problems of high cost and wide application, lack of safe and effective delivery system for gene drugs, etc., and achieve high-efficiency transfection , high transfection efficiency
CN101597349AInactive Publication Date: 2009-12-09常熟紫金知识产权服务有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
常熟紫金知识产权服务有限公司
Publication Date
2009-12-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a phenyloboricacid-modified cationic polymer, the structural formula is that (see lower right): wherein R is H or alkyl group containing one to ten carbons or substitution alkyl group containing nitrogen, oxygen, sulfur, chlorine, bromine or iodine; P is cationic polymer, the molecular weight Mw of P is from 400 to 500000, and n is more than or equal to 1 and less than or equal to 20. The preparation method is that: the phenyloboricacid with active groups and the cationic polymer are dissolved in methyl alcohol in N, N-dimethyl fomamide, and are reacted for 2-48 hours at the temperature of 20-60 DEG C for obtaining the phenyloboricacid-modified cationic polymer. The cationic polymer utilizes functional groups such as hydroxide radical and amino-group and the like in biomacromolecules such as phenyloboricacid group energy, protein, amylase, nucleic acid and the like to form reversible covalent-binding property, and improves the capacity of gene combination and cell carrying of carrier materials, so as to realize high-efficiency transfection of genes.
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Description

technical field

[0001] The invention relates to a cationic polymer modified by phenylboronic acid, its synthesis method and its application as a non-viral gene carrier. Background technique

[0002] In recent years, genetic engineering has made remarkable achievements all over the world. This technology is to modify and recombine the gene of the organism by artificially "cutting" and "splicing" the DNA molecule outside the organism, and then introduce it into the recipient cell for asexual reproduction, so that the recombinant gene can be reproduced in the recipient cell. expression to produce the gene product required by humans.

[0003] The application of genetic engineering in agricultural and animal husbandry production is mainly to cultivate new varieties of animals and plants with high yield, high quality or special purpose. Through genetic engineering technology, high-yield, stable yield and high-quality crops can be obtained, which can speed up the cultivation of n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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