87results about How to "Free from degradation" patented technology

A method of dry etching an etching layer that coats a surface of a GaN based semiconductor layer or a SiC, the method includes performing a first plasma etching to remain a desired thickness of the etching layer, and performing a second plasma etching on a region remained by the first plasma etching with a lower energy than that of the first plasma etching to expose a surface of the GaN based semiconductor layer or the SiC. The method of dry etching of the present invention is capable of suppressing the damage on the GaN based semiconductor layer and thereby achieving the dry etching method of the high selectivity, high anisotropy, low contamination, and low damage. It is thus possible to achieve the GaN based semiconductor device having excellent initial device characteristics and free from degradation due to conduction.

A novel nano polycation transgenic vector relates to a nano polycation transgenic vector of a cyclodextrin-polyethyleneimine-folacin ternary assembly structure which takes the cyclodextrin as a skeleton material and polymerizes the cyclodextrin after activated with small molecular polyethyleneimine(PEI) as well as couples the decorative folacin on the cyclodextrin-polyethyleneimine to manufacture the nano polycation transgenic vector with tumor targeting. The material composing method of the vector is simple and effective with a high recycling rate; the material is shown to have the characteristics of low poison and high transfecting efficiency by the filtering of a plurality of tumor cell strains and experiences in the body of a rat.

This invention discloses an ultrasonic microbubble contrast agent of carrier gene, it includes microbubble, fluorocarbon gas, gene or medicine. Microbubble wall is made up by lipid bielement, fluorocarbon gas is encapsulated in it, gene or medicine entrapped in microbubble envelope. This invention also discloses its preparation method, two stearyl phosphatidylcholine, two palmitoyl phosphatidyl ethanolamine, sapn-60, glycerol, phosphate buffer solution and gene or medicine is mixed as proper proportion for mechanical oscillations. The property of carry gene or medicine microbubble is stable, particle diameter disperses equally, local tissue medicine concentration can be obviously advanced though effect of transonic breaking microbubble, and curative effect is advanced and side effect can be reduced. Flushing and dilution of blood to gene and medicine can be prevented when used in body, and also prevent gene or medicine from degrading by nuclease in cycle to ensure that lot of gene and medicine can be transported to target tissue. And it is linear increase follow gene or medicine input amount in certain range.

The invention discloses an expression vector and an anti-tumor reagent of long-chain non-coded RNALOC401317, and applications of the expression vector and the anti-tumor reagent, namely the applications in preparation of a preparation for inhibiting growth and proliferation of tumor cells. According to a sequence of the lncRNA, a eukaryotic expression vector for overexpression of LOC401317 is designed and synthesized, and loaded on polylysine modified silicon nano-particles to prepare nano-spheres, so that the LOC401317 is successfully expressed in a nasopharynx cancer cell line and the growth of the nasopharynx cancer cells is inhibited. The polylysine modified silicon nano-particles mentioned in the invention can protect the LOC401317 vector from degradation of nuclease and prolong the action time, and thus a higher transfection efficiency is realized and further development and application are facilitated.

The invention discloses a magnetic targeting carrier capable of carrying genes and drugs, a preparation method and an application thereof. The invention is a carrier which has stability, safety and targeting and has controlled release behavior for non-viral magnetic gene therapy and drug therapy. The carrier material of the invention is characterized in that the carrier material is a bunchy silica mesoporous material with magnetism; the length-diameter ratio is not less than 3; the loading capability is big; the material has a protective effect on loaded genes and carriers and superparamagnetism, is not easy to agglomerate, and can control release speed of genes and drugs in vitro; and the surface thereof is easy to modify various functional groups, thus having wide adaptability. The invention also provides a preparation method of the carrier. When in use, therapeutic short chain DNA, siRNA or drugs enter in holes or are combined with surface modified functional genes by a soaking mode, then reach a targeted tissue by guidance of an applied magnetic field, and release the short chain DNA, siRNA or drugs carried thereby under the action of an alternating magnetic field, thus achieving the purpose of magnetic targeting controlled therapy.

The invention discloses a kit and virus sample preserving fluid capable of being used in a viral nucleic acid clinical detection procedure including a high-temperature inactivation step, and a viral nucleic acid clinical detection method using the sample preserving fluid or the kit. The sample preserving fluid, the kit and the clinical detection method have the advantages that the integrity and the quantity of virus nucleic acid can be ensured under the condition of utilizing high-temperature inactivated viruses, so that the virus nucleic acid can be effectively prevented from being degraded;and the detection accuracy of the virus nucleic acid in the clinical detection procedure is obviously improved.

The invention relates to a novel gene sequence of a recombinant chicken Alpha interferon, constructs a recombinant expression vector thereof and belongs to a gene engineering biological product obtained by a molecular biology method. The gene sequence of a newly designed chicken Alpha interferon is recombined into a pPICZ Alpha-A vector and then is confirmed on the special position of a microzyme by an electric conversion mode. Besides, a pichia expression system is adopted to express a foreign gene, thus being beneficial to the commercial production of the chicken interferon. The protein expressed by a gene group after being diluted by 4365158.3 times can completely restrain the attraction of vesicular stomatitis virus of 100-1000TCID50. Compared with a natural chicken Alpha interferon, the novel gene sequence of a recombinant chicken Alpha interferon has a higher anti-virus effect; the anti-virus effect thereof is improved by 8 times. Test also detects that the protein expressed by the gene can restrain the proliferation of a newcastle disease virus and an avian influenza virus; besides, the effect of the interferon with high concentration is more remarkable.

The invention relates to a polypeptide-protein-drug-carried solid particulate matter and a double-enteric solid preparation containing the same, and preparing methods and application of the polypeptide-protein-drug-carried solid particulate matter and the double-enteric solid preparation. The solid particulate matter contains polypeptide protein drugs, absorption enhancers and protease inhibitors.The prepared double-enteric solid preparation can resist degradation of gastric acid and gastric intestinal enzymes to the polypeptide protein drugs during oral administration and meanwhile has the colon-specific effect, and absorption of polypeptide proteins in an intestinal tract can be effectively promoted.

The invention relates to a modification method of functional plant polysaccharide, which comprises extracting functional polysaccharide from the plant with biological activity, separating, purifying and determining molecular weight and biological function, modifying structure via agent, activating active hydroxyl on sugar chain, connecting small molecule polyethylene imine, dialyzing, freezing and drying to obtain functional plant polysaccharide. The modification method has simple process, high efficiency and high yield, which keeps prior activity of natural polysaccharide and adds new biological function to obtain a natural macromolecule compound as non-viral gene carrier.

The invention discloses a cationic angelica polysaccharide nanoparticle gene delivery system. The system is a gene delivery system of angelica polysaccharide combined DNA (Deoxyribonucleic Acid) plasmids modified by amine compounds, wherein molecular weight distribution of angelica polysaccharides is 30 to 50KD and 80 to 100KD; the mass ratio of the cationic angelica polysaccharide to the DNA plasmids is (1-200):1; and the particle diameter of the cationic angelica polysaccharide-DNA plasmid nancomposite is 21 to 77nm. The system has the characteristics that: 1, the angelica polysaccharide has various biological activities such as immune regulation activity, anti-aging activity, anticoagulation activity and the like, is safe and biologically degradable, does not have immunogenicity and isprepared with a simple, economic and convenient process; and 2, all the three cationic angelica polysaccharides have good DNA plasmid combination effect and gene delivery expression effect. Positive charges carried by primary amine, secondary amine and tertiary amine groups combined with saccharide chains can be effectively combined with the DNA plasmids with negative charges through an electrostatic effect, so that the plasmids are protected from being degraded by various enzymes inside and outside cells.

The invention provides a modification method of polymer amino acid, in particular to a macromolecular compound of non-viral gene vector material, which is characterized in that the polymer amino acid is generated by polycondensation of amino acid monomer, the ring-opening of the polymer amino acid side group is processed by amino alcohol reagent, the structure modification is processed by the connection reagent, the polyethyleneimine (PEI) is joined by the activation of active hydroxy on the amino alcohol, and that the functional composite material of polymer amino acid-amino alcohol-polyethyleneimine is generated by dialysis and lyophilization. The modification method of polymer amino acid has the advantages of low mammalian toxicity, high gene transfection efficiency and performance of biodegradation.

The invention discloses cationized polysaccharide nanoparticle gene delivery systems, which are gene delivery systems combining polysaccharides modified by an amine compound and DNA plasmids, wherein the mass ratio of the cationized polysaccharides to the DNA plasmids is (0.5-200):1; the particle size of the cationized polysaccharide-DNA plasmid nano composites is 21 to 414 nanometers; and the amine compound is spermine, ethylenediamine or polyethyleneimine with a number-average molecular weight of 600Da to 2,000Da. The polysaccharides of traditional Chinese medicine universally have various bioactivities for immunoregulation, aging resistance, coagulation resistance and the like. Compared with other viral vector and other non-viral vector gene delivery systems, the cationized polysaccharide nanoparticle gene delivery systems are safe, free from immunogenicity and biodegradable, and the preparation process of the cationized polysaccharide nanoparticle gene delivery systems is simple and economic. The cationized polysaccharides have good DNA plasmid bonding actions and gene delivery and expression functions. The positive charges on the aminos bonded with the polysaccharide chains can effectively combine with the negative charges on the DNA plasmids to protect the plasmids from being degraded by various enzymes in and out cells.

The invention relates to a double auxotrophic yeast, wherein the yeast is Hansenula polymorpha, and the orotic glycoside-5-phosphate decarboxylase gene and the beta-isopropyl malate dehydrogenase gene of the Hansenula polymorpha are blocked. The invention also relates to a preparation method of the double auxotrophic yeast. In addition, the invention also provides a DNA sequence and a recombinant vector used to prepared the double auxotrophic yeast. The double auxotrophic yeast of the invention has the advantages of low reverse mutation, high genetic stability, high biomass, and the like, and plays an important role in genetic engineering vaccine production; for example, HPV16-type L1 and 58L2 protein have double expression with high efficiency in the yeast.

The invention relates to a multifunctional synergistic pharmaceutical composition based on adriamycin. According to the pharmaceutical composition, natural hydrophobic small molecules having a conjugated structure are covalently coupled with a polysaccharide skeleton to form an anti-angiogenesis drug, the anti-angiogenesis drug is physically mixed with the conjugated structure-modifying mitochondria damage peptide derivative and adriamycin, and the pharmaceutical composition of a nano size is assembled by virtue of various supramolecular driving forces. The pharmaceutical composition has the advantages of simultaneously regulating a tumor micro environment and tumor cells, reversing the anti-apoptosis characteristics of tumor cells, and maximizing the antitumor effect of the adriamycin. Inaddition, the multifunctional synergistic pharmaceutical composition has the advantages of the adriamycin such as high load, high stability and high targeting. The multifunctional synergistic pharmaceutical composition based on the adriamycin is compatible with corresponding medicinal auxiliary materials to prepare antitumoar drug preparations for injection, oral administration or external use. The multifunctional synergistic pharmaceutical composition is prepared by virtue of a multi-component supramolecular combination construction, so that the operation is simple, and the industrialized production is easy to realize.

The invention discloses an immune nano-carrier for conveying siRNA (small interfering Ribonucleic Acid). An active ingredient of the immune nano-carrier is a coupling product of a single-chain antibody and a siRNA conveying carrier; and the siRNA conveying carrier is polyethylene glycol-polyethylene imine-superparamagnetic ferric oxide nanoparticles formed by coupling superparamagnetic ferric oxide nanoparticles with polyethylene glycol-polyethylene imine. Moreover, the invention further discloses a preparation method of the immune nano-carrier. In the immune nano-carrier, the single-chain antibody is coupled with the siRNA conveying carrier, so that the immune nano-carrier has the physicochemical properties of small particle size and appropriate surface potential, high siRNA compounding capability, low cell toxicity, high transfection efficiency, high targeted conveying characteristic, and good application prospects on siRNA conveying and RNA interference-based disease treatment.

The invention provides an alpha helix cationic polypeptide as well as a preparation method and application of the alpha helix cationic polypeptide. The alpha helix cationic polypeptide takes a photosensitizer porphyrin as a core and alpha helix polypeptides as four arms, and a nucleotide drug prepared from the polymer can be applied to a nucleotide drug delivery system. The polymer provided by theinvention has good biocompatibility, low cytotoxicity and light activation ability. The polymer provided by the invention is self-assembled in a water solution to form nanoparticles which has good stability, biocompatibility and low cytotoxicity; the preparation method is simple and high in repeatability, and the polymer serving as a carrier is not only capable of protecting nucleic acid such asDNA from being degraded, but also capable of being combined with the scale effect of the nanoparticles so as to be used for treating diseases; and in addition, the transfection promoting effect for different cancer cells can be achieved by virtue of the light activation ability.

The invention discloses a lipid nanoparticle preparation and application thereof. The lipid nanoparticle preparation comprises lipid nanoparticles and a buffer system; the lipid nanoparticles are obtained by encapsulating at least one RNA molecule for encoding an antigen with non-load nano lipid; and the non-loaded nanolipid comprises cationic lipid and neutral lipid at least selected from the combination of DOPE and cholesterol. The three-component combination preparation adopted by the invention is obviously superior to a two-component combination preparation when mRNA transfection is carried out in vitro. According to the invention, compared with a nano-lipid formed by a cationic lipid and a neutral lipid, the three-component lipid nano-particle formed by combining two neutral lipids and the cationic lipid in a specific ratio shows higher transfection efficiency and mRNA expression efficiency in the mRNA coating and in-vitro cell transfection processes.

The present application relates to an isolated chimeric molecule comprising a degradation domain comprising an E3 ubiquitin ligase (E3) motif and a targeting domain capable of specifically directing the degradation domain to a substrate, where the targeting domain is heterologous to the degradation domain. A linker couples the degradation domain to the targeting domain. Also disclosed are compositions as well as methods of treating a disease, substrate silencing, screening agents for therapeutic efficacy against a disease, and methods of screening for disease biomarkers.

A semiconductor integrated circuit has a first substrate of a first polarity to which a first substrate potential is given, a second substrate of the first polarity to which a second substrate potential different from the first substrate potential is given, and a third substrate of a second polarity different from the first polarity. The first substrate is insulated from a power source or ground to which a source of a MOSFET formed on the substrate is connected. The third substrate is disposed between the first and second substrates in adjacent relation to the first and second substrates. A circuit element is formed on the third substrate.

The invention relates to a preparation method and application of a copper sulfide/hyperbranched macromolecular nano bionic enzyme. An intelligent nano enzyme system is constructed to realize pH-controllable chemical kinetics enhanced photo-thermal anti-tumor therapy so as to solve the problem of high recurrence of lethal cancer. According to the nano bionic enzyme system, a copper sulfide nano particle core is used as a photo-thermal agent and a Fenton-like catalyst, and a hyperbranched macromolecular polymer is used as a template to wrap copper sulfide and graft glucose oxidase. Research results prove that the prepared nano bionic enzyme system has excellent chemical kinetics and photo-thermal therapy performance in a slightly acidic environment of tumors, can efficiently inhibit growth and recurrence of the tumors, and provides a new strategy for clinical treatment of high-recurrence middle and advanced stage cancers.

The invention relates to a method for producing a nattokinase liposome from phytosterol. The method for producing a nattokinase liposome from phytosterol is characterized by comprising: (1) a step of raw material selection, in which lecithin, phytosterol, ether, a buffer solution and a solution of nattokinase are selected in mass ratio of 100-300 mg:90-200 mg:2-5 mL:5-10 mL:5-10 mL for later use; 2) a step of blank liposome preparation, in which the lecithin, the phytosterol and the ether are mixed, the mixture is subjected to rotary evaporation to be formed into a uniform lipoid membrane to cover the internal bottom of a pear-shaped flask, the buffer solution is added into the flask, glass beads are added into the flask, the flask is rotated and shaken at 60 DEG C for 30 to 90 minutes, the mixture in the flask is treated by ultrasonic wave in a water bath for 10 to 20 minutes, and the materials in the flask is subjected to freeze drying to form a precursor substance of the liposome; and 3) a step of hydration, in which the solution of nattokinase is added into the precursor substance of the liposome for hydration in nitrogen flow for 10 to 20 minutes and thus the nattokinase liposome is obtained. The method is simple in process and can reduce the influences of cholesterol on human body.

The present invention relates to uses of modified chitosan as a protein polypeptide drug carrier, wherein the modified chitosan has a structural unit represented by the following formula defined in the specification, R in each structure unit can be the same or different, and respectively and independently are hydrogen, acetyl, or the residue obtained after an amidation reaction between a cholic acid-based compound and the amino group in a raw material chitosan, the weight average molecular weight of the raw material chitosan is 1000-1000000, the deacetylation degree is 70-95%, and the substitution degree of the cholic acid-based compound in the modified chitosan is 0.5-20%. The invention further relates to a nanometer complex containing the modified chitosan, a preparation method and uses thereof, wherein the nanometer complex is mainly used for promoting the oral absorption of protein polypeptide drugs, and can well improve the biological treatment effects of protein polypeptide drugs.