Magnetic targeting carrier capable of carrying gene and drug, preparation method and application thereof
A carrier and drug technology, which is applied to a magnetic targeting carrier that can carry genes and drugs and its preparation and application fields, can solve the problems of host immune response, low expression efficiency and the like
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Embodiment 1
[0060] Add 5.2 g of CTAC cationic surfactant and 1.2 g of P123 anionic surfactant to 7.72 ml of tetraethyl orthosilicate ethylene glycol solvent, and 20 g of hydrochloric acid solution at PH=2, mix well and stir at 3000 RPM for 30 hours , add 5ml of concentrated ammonia water, stir at high speed for 5 minutes, and then age for two days, filter, dry at room temperature and sinter at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.
Embodiment 2
[0062] Add 4.6 grams of CTAB cationic surfactant and 2.3 grams of F127 anionic surfactant to ethanol solvent containing 8.15ml tetraethyl orthosilicate, and 20 grams of hydrochloric acid solution with pH=2.5, mix well and stir at 3000RPM for 30 hours at high speed, add 5ml of concentrated ammonia water was stirred at high speed for 5 minutes, aged for two days, filtered, dried at room temperature and sintered at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.
Embodiment 3
[0064] Add 4.9 grams of CTAB cationic surfactant and 1.7 grams of P123 anionic surfactant to a solvent containing 10.22 ml of tetraethyl orthosilicate isopropanol, and 20 grams of hydrochloric acid solution at pH=2, mix well and stir at 3000 RPM for 30 hours , add 5ml of concentrated ammonia water, stir at high speed for 5 minutes, and then age for two days, filter, dry at room temperature and sinter at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.
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