Layered bimetal hydroxide / selenium nanocomposite and application thereof

A layered bimetallic, hydroxide technology, applied in the direction of drug combination, sulfur/selenium/tellurium active ingredients, genetic material components, etc., can solve the problems of unsatisfactory cellular absorption effect of nano-selenium, limited clinical application, etc. Promote orderly release, efficient gene silencing effect, inhibit cell proliferation effect

Inactive Publication Date: 2015-10-28
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cellular uptake of nanoselenium is not ideal, which severely limits its clinical application.

Method used

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  • Layered bimetal hydroxide / selenium nanocomposite and application thereof
  • Layered bimetal hydroxide / selenium nanocomposite and application thereof
  • Layered bimetal hydroxide / selenium nanocomposite and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of layered double hydroxide / selenium nanocomposite SeLDH

[0032] Mg(NO 3 ) 2 (3mmol) and Al(NO 3 ) 3 (1mmol) was dissolved in 10mL deionized water, and quickly added to 40mL NaOH solution (6mmol); the mixture was stirred at room temperature for 10min, and centrifuged (5min, 4500min -1 ) to collect the resulting slurry; wash twice with 40mL deionized water, centrifuge and resuspend in 50mL deionized water; transfer the resulting inhomogeneous suspension to a reactor and heat at 100°C for 16h to obtain a transparent, uniform The layered double metal hydroxide nano sol; the sol contains nearly 4mg / mL uniformly dispersed MgAl-LDH nanoparticles;

[0033] A certain amount of Na 2 SeO 3 The solution (1mL, 1M) was added dropwise to the hydrated solution of MgAl-LDH nanoparticles (2mL, 0.025M), and the stirring was continued for 30min; then, L-cysteine ​​(L-Cys) was added to the mixture, and the Stir until the reaction solution becomes deep red; the...

Embodiment 2

[0034] Example 2 Preparation of layered double hydroxide / selenium nanocomposite SeLDH-siRNA

[0035] Incubate 100nM siRNA and different masses of SeLDH in phosphate buffer (PBS, pH=7.4) for 30min at room temperature (25°C) to obtain SeLDH-siRNA with different mass ratios, wherein the mass ratios of SeLDH and siRNA were respectively 1:1, 2:1, 4:1, 8:1 and 16:1;

[0036] The siRNA is selected from PsiRNA, TsiRNA or MocksiRNA; SeLDH-PsiRNA, SeLDH-TsiRNA or SeLDH-MocksiRNA is obtained.

[0037] The siRNA is selected from the mixture of PsiRNA and TsiRNA to obtain SeLDH-pooled siRNAs, wherein the molar ratio of PsiRNA and TsiRNA is 1:1.

Embodiment 3

[0040] Example 3 SeLDH gene load test

[0041] Agarose gel electrophoresis was used to detect the ability of SeLDH and MgAl-LDH to bind siRNA. Different mass ratios (1:1 to 16:1) of SeLDH / siRNA or LDH / siRNA were prepared in PBS, and electrophoresed by TBE buffer containing 1% agarose for 1 h. The gel was stained with 1 μg / ml ethidium bromide and photographed under a UV imager, see figure 2 . figure 2 A shows that when the mass ratio exceeds 4:1, the migration of LDH-bound siRNA is completely blocked, indicating that LDH can fully bind siRNA and protect it from degradation during electrophoresis. The binding ability of SeLDH is relatively weak, figure 2 It is shown in B that it is not completely blocked until the mass ratio is 8:1. Compared with LDH, SeLDH does not bind siRNA so tightly that it is not easy to release, thus exerting a greater biological effect.

[0042] To detect the release of siRNA, SeLDH / siRNA (16:1) and LDH / siRNA (16:1) were incubated in phosphate bu...

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Abstract

The invention relates to the technical field of nano-selenium preparation, and particularly discloses layered bimetal hydroxide / selenium nanocomposite and application thereof. The layered bimetal hydroxide / selenium nanocomposite is prepared through the following steps that selenium salt solution is dropwise added into layered bimetal hydroxide LDH hydration solution, stirring is conducted, then reducing agent is added, stirring is conducted continuously until the reaction liquid turns into crimson, then centrifugation, washing and drying are conducted on the product, and the layered bimetal hydroxide / selenium nanocomposite Se@LDH is obtained. The selenium nanocomposite can be used as a transmitting system for siRNA to promote the orderly release of the siRNA, and at the same time, the concentration of intracellular drug is increased, so that the sensibility of a drug-resistant cell to taxol is recovered.

Description

technical field [0001] The invention relates to the technical field of nano-selenium preparation, in particular to a layered double metal hydroxide / selenium nanocomposite and its application. Background technique [0002] Microtubules (MTs), as major components of the eukaryotic cytoskeleton, are involved in a wide range of cellular processes. Microtubules play an important role in a variety of cellular functions and are therefore targeted intracellularly for antineoplastic drugs such as taxanes and vinblastine. These microtubule-binding agents disrupt the normal formation of the mitotic spindle and subsequently induce apoptosis. Taxanes (taxotere and paclitaxel) represent an important class of antineoplastic drugs targeting microtubules, which can be used for in vivo and in vitro therapy of lung cancer, ovarian cancer, breast cancer and leukemia. However, the non-response or failure of re-chemotherapy after the first action of taxane drugs promotes the intrinsic or acquir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K47/48A61K47/04A61K48/00A61K31/7088A61P35/00
Inventor 刘杰郑文静周艳晖陈兰美
Owner JINAN UNIVERSITY
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