Preparation method and application of copper sulfide/hyperbranched macromolecular nano bionic enzyme

A technology of macromolecules and copper sulfide, applied in the field of anti-tumor, can solve the problems of insignificant influence of chemical kinetics on tumor recurrence, insufficient amount of endogenous hydrogen peroxide, easy tumor recurrence, etc., and achieve enhanced photothermal therapy effect , good catalytic activity, and high light-to-heat conversion efficiency

Active Publication Date: 2020-12-18
WENZHOU MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0005] In order to solve the major clinical challenges of the existing technology, such as easy recurrence and refractory tumors, as well as the insufficient amount of endogenous hydrogen peroxide available in residual tumor cells after photothermal therapy, the effect of chemical kinetics on tumor recurrence is not significant Deficiencies, the present invention provides a preparation method and application of copper sulfide / hyperbranched macromolecular biomimetic enzyme, which can inhibit the growth of most tumors through efficient near-infrared response photothermal effect, and further catalyze chemical kinetics through Fenton It can effectively inhibit the recurrence of tumors, and provide a new strategy for the clinical treatment of cancer to treat both the symptoms and the root causes.

Method used

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  • Preparation method and application of copper sulfide/hyperbranched macromolecular nano bionic enzyme
  • Preparation method and application of copper sulfide/hyperbranched macromolecular nano bionic enzyme
  • Preparation method and application of copper sulfide/hyperbranched macromolecular nano bionic enzyme

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Embodiment 1

[0031] Such as figure 1 As shown, the preparation of copper sulfide / hyperbranched macromolecular nanozyme includes the following steps: first, CuCl 2 • 2H 2 O and hyperbranched macromolecular polymers (such as the fifth generation polyamidoamine dendrimers—G5) are mixed in water at a certain ratio. After mixing by magnetic stirring, the Na 2 S•9H 2 O was added to the above solution, and stirring was continued at 30°C. The obtained product was transferred to a dialysis bag with a molecular weight cut-off of 1000 and dialyzed in distilled water for three days (4 L × 3) to obtain purified CuS@G5. Afterwards, the carboxyl group of mPEG-COOH was activated by EDC and NHS, and added to the above-mentioned purified CuS@G5 solution, and the stirring was continued for 24 h. The obtained product was transferred to a dialysis bag with a molecular weight cut-off of 1000, and dialyzed in distilled water for three days (4L×3) to obtain purified CuGP. Finally, the CuGP prepared above w...

Embodiment 2

[0033] Such as figure 2 As shown, the synthesized CuGP is a uniform nanoparticle with an average size of 25 nm. When the CuGP / G nanozyme is prepared by loading GOD, the shape and size of the obtained product are similar to CuGP, indicating that the loading of GOD does not affect the size of CuGP. .

Embodiment 3

[0035] Such as image 3 As shown, the prepared 0.1 mg / mL CuGP / G nanozyme of the present invention is at 808 nm (1.2 W / cm 2) after 5 min of laser irradiation, the solution temperature increased from the initial 29.5 °C to 43.1 °C. As the concentration of CuGP / G increases, the amplitude of temperature increase also increases. When the concentration of CuGP / G was increased to 1.5 mg / mL, the temperature increased to 68.1 °C within 5 min, indicating that the prepared CuGP / G had excellent photothermal properties.

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Abstract

The invention relates to a preparation method and application of a copper sulfide/hyperbranched macromolecular nano bionic enzyme. An intelligent nano enzyme system is constructed to realize pH-controllable chemical kinetics enhanced photo-thermal anti-tumor therapy so as to solve the problem of high recurrence of lethal cancer. According to the nano bionic enzyme system, a copper sulfide nano particle core is used as a photo-thermal agent and a Fenton-like catalyst, and a hyperbranched macromolecular polymer is used as a template to wrap copper sulfide and graft glucose oxidase. Research results prove that the prepared nano bionic enzyme system has excellent chemical kinetics and photo-thermal therapy performance in a slightly acidic environment of tumors, can efficiently inhibit growth and recurrence of the tumors, and provides a new strategy for clinical treatment of high-recurrence middle and advanced stage cancers.

Description

technical field [0001] The invention relates to the technical field of preparation of nano biomimetic enzyme and its application in anti-tumor fields such as photothermal and chemical kinetic combined therapy, in particular to a preparation method and application of copper sulfide / hyperbranched macromolecular nano biomimetic enzyme. Background technique [0002] As one of the three major diseases in the world, cancer has a very high fatality rate, and the annual number of patients shows a rapid upward trend. It is a major disease that seriously threatens the survival of human beings. Studies have confirmed that the cure rate of early cancer is high, while advanced cancer has a higher recurrence rate and mortality. Even with successful treatment, the survival rate for advanced cancer is extremely poor. Conventional surgical treatment of cancer is more traumatic to the human body and can easily cause a variety of surgical complications. As a local treatment, it is more suitab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K38/44A61K47/32A61K47/34A61K47/60A61P35/00B82Y5/00
CPCA61K41/0052A61K38/443A61K47/32A61K47/34A61K47/60C12Y101/03004A61P35/00B82Y5/00A61K2300/00Y02A50/30
Inventor 孔令丹刘勇袁峰黄萍萍晏露
Owner WENZHOU MEDICAL UNIV
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