37results about How to "Particle rules" patented technology

The invention provides EV71virus-like particles as well as preparation method and application of the EV71virus-like particles. The method comprises the following steps of: connecting a P1 protein gene and 3CD protease gene of an EV71 virus with predicated mean vote (PMV) plasmid to build PMV-P1-3CD recombinant expression plasmid; turning into hansenula polymorpha AU-0501 expression bacterial strain to obtain an AU-PMV-P1-3CD recombinant expression bacterial strain; fermenting and culturing the recombinant expression bacteria strain and performing inducible expression on EV71 virus-like particle proteins by using methanol; centrifugally gathering thallus to perform homogenate crushing at high pressure; and purifying the supernate through ion-exchange column chromatography, hydrophobic chromatography and sieve chromatography to obtain the EV71virus-like particles. EV71virus-like particle vaccines provided by the invention has high immunogenicity, security, immunological characteristics and biological activity; and the EV71virus-like particles can be prepared in a large scale and purified and used for preparing vaccines for preventing EV71 infection, and has good economic value and application prospect.

The invention discloses a method for milling by low temperature low dew point gas spray freeze drying which uses an atomizer for nebulizing liquid to be dried into 1 to 250 micron droplets which are frozen into ice powder in the gas with the low temperature of -60 DEG C and the dew point of -70 to -80 DEG C, then air-intake temperature is adjusted to 2 to 4 DEG C lower than the eutectic temperature of materials, the gas passes through a pore plate of a fluidized bed to lead the ice powder which stays on the fluidized plate to be fluidized, and simultaneously the solvent in the ice powder is sublimated. The sublimation drying process is completed until the dew point of the gas at the gas vent returns to -55 to -60 DEG C. The ice powder is dried for 1 to 2 hours into powder products when the temperature is increased to 30 to 50 DEG C. The gas coming out of the gas vent returns to the place for low temperature low dew point gas preparation for recirculation. The method is characterized by easy operation, continuous duty, and the like, and is especially suitable for the drying of materials such as medicine, food, biochemical product, and the like, guarantees the quality of products and can directly obtain the dried powder products; simultaneously the drying time of the method is greatly shortened compared with the vacuum freeze drying.

The invention provides a high-performance lithium ion battery composite positive electrode material and a preparation method thereof, wherein the high-performance lithium ion battery composite positive electrode material comprises a high-nickel ternary positive electrode material matrix and a Bi2-aMaWO6 coating layer coating the high-nickel ternary positive electrode material matrix, and a is greater than or equal to 0 and less than 1, and M is any one or a combination of two or more of Zr, Mg, Al, Na, Fe, Ga, Mo, W, Y, Ti and Ce. According to the preparation method, the Bi2-aMaWO6 modified high-nickel ternary positive electrode material is obtained mainly through the high-temperature calcination of Bi2-aMaWO6 and the high-nickel ternary positive electrode material, the preparation methodis simple, and the prepared modified positive electrode material is regular in particle morphology, good in crystallinity and free of impurity phases; the generation and expansion of micro-cracks canbe successfully inhibited in the charging and discharging process, so that the harmful phase change from H2 to H3 is favorably reduced; and meanwhile, the specific discharge capacity, the cycle performance and the thermal stability of the positive electrode material are improved.

The invention relates to an electron donor of a Ziegler-Natta catalyst, a catalyst component comprising the electron donor and the catalyst. The electron donor adopts a Si-Al ester compound; the catalyst prepared from the Si-Al ester compound serving as the electron donor shows higher catalytic activity, better hydrogen response and better copolymerization performance when applied to vinyl polymerization and copolymerization, and can be used for catalyzing vinyl homopolymerization or production of high-performance polyolefin materials with high bulk density, narrow particle size distribution and small fine grain content through copolymerization of vinyl and other alpha-olefins.

The invention provides hansenula polymorpha engineering bacteria for efficiently expressing CA10 virus-like particles and application thereof. The engineering bacteria comprise a recombinant vector carrying CA10 virus P1 and 3CD genes, and an original strain of the engineering bacteria is uracil-deficient hansenula polymorpha AU 0501; and the P1 and 3CD genes are optimized according to hansenula polymorpha preferred codons. The invention further provides a preparation method of the CA10 virus-like particles. The preparation method comprises the steps that the engineering bacteria are cultured,the CA10 virus-like particles are expressed, and the virus-like particles are separated and purified by adopting ultrafiltration and three-step chromatography processes. The CA10 virus-like particlesand a vaccine prepared from the CA10 virus-like particles have good immunogenicity, safety and biological activity, the process is simple, purification is conducted by adopting a chromatography method, large-scale preparation and purification can be achieved, a high-purity (greater than 99%) VLP protein stock solution is obtained, and the engineering bacteria can be used for preparing the vaccinefor preventing CA10 infection and have good economic value and application prospects.

The invention discloses a method for preparing high purity nano cobalt hydroxide, belonging to the technical field of battery energy material preparation. Homogeneous phase precipitation method is adopted to synthesize cobalt hydroxide, including that: cobaltous sulphate aqueous solution, sodium hydroxide aqueous solution, cobalt ion chelating agent aqueous solution and aqueous solution of reagent A which are in certain concentration are respectively, sequentially and continuously added into a reactor to be stirred according to flow ratio of 1: 0.68-0.74: 0.048-0.054: 0.058-0.064, reaction is taken place, appropriate amount of additive aqueous solution is added, and ageing, solid-liquid separation, washing and drying are carried out, thus obtaining the high purity nano cobalt hydroxide product. The product not only has high purity, regular shape, small grain size and uniform distribution, but also has high specific area, higher bulk density and good liquidity, dispersity and workability, thus being beneficial to separate processing or uniformly coating cobalt hydroxide on the surface of nickel hydroxide particle by adopting chemical plating, so that combination property of nickel-hydrogen battery is improved.

The invention discloses a preparation method of tri-phosphor double-peak fluorescent powders used for an energy-saving lamp, which has high heat stability and high brightness. The preparation method of the tri-phosphor double-peak blue fluorescent powders for the lamp comprises the steps of firstly weighing each chemical component according to a chemical expression of fluorescent material, then adding BaF2 being 0.1wt%-10.0wt% of the total weight of all chemical components, and CaF2 being 0.01wt%-4.0wt% of the total weight of the all chemical components, sufficiently and uniformly mixing the raw materials, and then burning and reacting sufficiently at 1350-1500 DEG C under the atmosphere of reduction gas and inert gas to obtain a final burning product, then grinding, washing, filtering, drying and screening the burned product to obtain the tri-phosphor double-peak blue fluorescent powders for the lamp. Due to the adoption of the method, the blue fluorescent powders with regular particle morphology, uniform particle size, relatively high brightness and high heat stability, and according to the preparation method, the preparation process is simple, a one-step reduction method is adopted, and the industrial large-scale production is facilitated.

The invention relates to a preparation method for potash fertilizer particles. The preparation method is characterized in that the potash fertilizer particles are pelletized by a two-stage mixing extruder, potash fertilizer powder and additives are rapidly refined, scattered and mixed due to the strong shear force of the high rotation speed screw of a first-stage twin screw mixing mill, and then enter a second-stage single screw extruder through exhaust, the output end can conduct high pressure stable extrusion at a low rotation speed, and finally, pelleting is carried out by a die surface pelletizer to obtain the potash fertilizer particles. The potash fertilizer particles have high nutrient concentration, are regular in shape and uniform in diameter, and have high strength and good slow release performance. The preparation method is simple to operate, has strong technological adaptability, consumes little energy, has high granulation rate and high yield, and has market application value.

A jujube granule producing method is disclosed. The method includes raw material selecting, cleaning, dehydrating, stoning, sterilizing, enzymolysis, fermentation, low-oxygen low-temperature heat-pump drying, nitrogen charging pressure difference rapid puffing, medium and short wave infrared drying, crushing, grading, packaging, and other steps. The enzymolysis is performed firstly so that jujube viscosity is reduced and jujube flavor is adjusted. Then through progressive multi-stage drying, the color of jujubes is kept, a proper tissue structure is formed, the water content is controlled ingeniously. Through combining a crushing manner, the rotating speed, environment temperature and humidity, and other factors, the problem that jujubes are liable to stick to form clump or liable to be powdered, and the like in granulation is overcome by and objectives of improving uniformity, reducing viscosity, maintaining the original color, increasing the crisp degree, making granulation easy and avoiding powder generation are achieved. The method is simple and convenient to operate, high in controllability and high in mechanization degree. The utilization rate, edibility and healthcare properties of raw materials are improved. A product of the method can be used for snacks, soup making, medicines, tea drinks and stuffing.

The invention discloses a method for preparing an active material calcium zincate of a cathode of a battery by utilizing an airflow mill and belongs to the technical field of an active material of a zinc nickel battery. The method comprises the following steps: 1) uniformly mixing and stirring zinc oxide, calcium hydroxide and deionized water at a weight ratio of (4-6):(4-6):1, and then putting the mixture into a material inlet of the airflow mill and entering into a crushing chamber through an ascending pipe, thereby acquiring a crushed material; 2) putting the crushed material acquired in the step 1) into a classifying chamber for classifying, thereby acquiring classified particles; and 3) returning the classified particles acquired in the step 2), of which the grain size is less than or equal to 1000 meshes, to the crushing chamber for crushing, and discharging the classified particles of which the grain size is more than 1000 meshes from a product outlet, namely, acquiring the active material calcium zincate of the cathode of the battery. The calcium zincate product acquired according to the method is uniform in fineness, regular in particle shape, high in purity and high in material activity. The charging / discharging cycle life can reach above 3000 times.

The invention provides a foot-and-mouth disease virus-like particle vaccine and a preparation method thereof. Insert the two ORFs of the codon-optimized FMDV P12A and 3C genes in series into the transfer vector to construct a recombinant expression plasmid; or clone a single ORF containing the P12A3C gene under the promoter SV40 into the vector to construct a recombinant expression plasmid, and transform competent cells to obtain recombinant expression Bacmid‑DNA was transfected into sf9 cells to obtain insect cells capable of expressing foot-and-mouth disease virus-like granule protein. After culturing the cells, the supernatant is collected, concentrated by ultrafiltration, centrifuged, and chromatographically purified to prepare the VLP vaccine.

The invention discloses a preparation method for regular particle-shaped aluminate green emitting phosphor. The method comprises the following steps of: weighing raw materials according to the stoichiometric proportion of all chemical components in an expression (Ce0.63Tb0.37)MgAl11019; weighing a H3BO3 fluxing agent, a Li2CO3 fluxing agent and a AlF3 fluxing agent which are used as composite fluxing agents, and adding into raw materials and uniformly mixing; placing raw material powder in a crucible and calcining to obtain a primary calcined product; crushing the primary calcined product, placing in the crucible again, and calcining in the atmosphere of H2 / N2 to obtain a secondary calcined product; and crushing the secondary calcined product, washing, filtering, drying and screening to obtain a final product. According to the preparation method, (Ce,Tb)MgAl11O19 (CMAT) green emitting phosphor which is regular in particle shape, uniform in particle size and high in relative luminance is obtained on the basis of the conventional high-temperature solid phase method by mainly adjusting the proportion of all the fluxing agents in the composite fluxing agents; and the preparation process is simple, so that the green emitting phosphor can be industrially produced on large scale.

The invention discloses a method for synthesizing copper-rare earth nanometer petal-shaped metal oxides, which comprises: weighing copper and rare earth soluble salt compounds in deionized water according to a molar ratio of 0.3 to 2:1, and dissolving them in deionized water at 0 to 90 Stir at ℃ for 10-90 minutes; add coordination solvent, continue to stir, and let the solution stand for 30-120 minutes to obtain an acidic precursor; add a neutralizing precipitant to the acidic precursor solution to make the solution If stratification occurs, add the coordination solvent again, and repeat the operation from the beginning of stirring to the end of standing until the solution appears stratified; After 3-5 hours, a metal oxide material with a petal-like lamellar structure is obtained. The invention realizes the mixing of materials at the atomic size, and can accurately control the atomic ratio; after low-temperature sintering, a copper-rare earth nano-petal-like sheet structure with high purity and uniform sheets composed of nanoparticles is prepared.

The invention discloses a novel micron-scale up-conversion luminescent material, which is a compound with the following chemical formula: NaYF 4 : Yb, Ln, has the advantages of regular particle shape, uniform particle size, and good dispersibility; the present invention also discloses a novel preparation method for preparing micron-scale up-conversion luminescent materials, using impinging flow-precipitation method, the process involved The process is simple, the energy consumption is small, and the product yield is high, the particle size is small and uniformly distributed, and the luminous intensity is good, which can effectively solve the problems of low yield, complex process flow and poor dispersion of up-conversion luminescent materials in the prior art. The method solves the problems that current up-conversion luminescent materials are difficult to mass-produce, and is suitable for popularization and application.

The invention relates to an electron donor of a Ziegler-Natta catalyst, a catalyst component comprising the electron donor and the catalyst. The electron donor adopts a Si-Al ester compound; the catalyst prepared from the Si-Al ester compound serving as the electron donor shows higher catalytic activity, better hydrogen response and better copolymerization performance when applied to vinyl polymerization and copolymerization, and can be used for catalyzing vinyl homopolymerization or production of high-performance polyolefin materials with high bulk density, narrow particle size distribution and small fine grain content through copolymerization of vinyl and other alpha-olefins.

The invention provides a preparation method for nanometer Bi2Ti2O7 powder. The preparation method comprises the following steps: completely dissolving Bi(NO3)3.5H2O and acetic anhydride so as to obtain a solution A; then dissolving tetrabutyl titanate in glycol and adding glacial acetic acid so as to obtain a solution B; mixing the solution A with the solution B and drying the obtained mixture in a baking oven; and carrying out calcining, insulation and cooling so as to obtain the Bi2Ti2O7 powder. The prepared Bi2Ti2O7 powder has improved photocatalytic performance and the characteristics of completely developed powder crystal phase, high purity, regular particle morphology, a size of less than 100 nm, uniformness and fineness.

The invention discloses a fine developer for large-format development and a preparation method thereof, which comprises 75-95% by mass of a bisphenol A crystalline polyester resin binder, 2-15% of an inorganic dye, 1-5 % of olefin oligomer, 0.5-5% of charge regulator, 0.2-5% of charge stabilizer, etc. are fully mixed and then added to a twin-screw extruder for high-temperature extrusion dispersion. The extrusion dispersion temperature is 100-150°C. Fine developer particles of 6-8 μm are obtained by pulverization and classification, and then 0.5-5% developer fluidization agent is mixed and distributed on the surface of the fine developer particles to finally produce large-format fine developer materials. The fine developing material particles obtained by the above method are regular, fine and uniform up to 6-8 μm, have high charge and stability at 20-30 μc / g, and good fluidity above 70%.

The present invention provides a human papilloma virus 16 type L1 protein HPV16L1-g and a coding gene and application thereof. The amino acid sequence of the HPV16L1-g protein is shown as SEQ ID No. 2, and the coding gene is shown as SEQ ID No. 1 in a sequence table. The present invention also provides an HPV16L1-g recombinant expression vector. The HPV16L1-g protein provided by the present invention has good immunogenicity, no potential carcinogenic risk, has good safety, immunological characteristics and biological activity, can be prepared and purified in a large scale, can be used for preparing a vaccine for preventing cervical cancer and drugs for treating cervical cancer, and has good economic values and application prospects.

The invention relates to a preparation method of a lithium iron phosphate / lithium vanadium phosphate composite material. The method comprises the following steps: mixing iron phosphate and vanadium pentoxide which are utilized as raw materials with a lithium salt compound; adding a chelating agent; grinding and mixing; performing heat treatment in an inert atmosphere, and then adding a carbon source material; and heating, roasting and cooling to room temperature to obtain the carbon-coated lithium iron phosphate / lithium vanadium phosphate composite material. The lithium iron phosphate / lithiumvanadium phosphate composite material can be assembled into a testing battery of which the discharge specific capacity can reach 144mAh / g with the current of 150mA / g at room temperature and can reach105mAh / g with the current of 30mA / g at the temperature of minus 20 DEG C, the crystal form is complete, the particle appearance is regular, and excellent normal-temperature and low-temperature electrochemical performances are shown. All the raw materials in the method are bulk industrial chemicals, the cost is low, the process route is simple, the industrial scale production is easy to realize and the application prospect is very broad.

The present invention provides a human papilloma virus 16 type L1 protein HPV16L1-h and a coding gene and application thereof. The amino acid sequence of the HPV16L1-h protein is shown as SEQ ID No. 2, and the coding gene is shown as SEQ ID No. 1 in a sequence table. The present invention also provides an HPV16L1-h recombinant expression vector. The HPV16L1-h protein provided by the present invention has good immunogenicity, no potential carcinogenic risk, has good safety, immunological characteristics and biological activity, can be prepared and purified in a large scale, can be used for preparing a vaccine for preventing cervical cancer and drugs for treating cervical cancer, and has good economic values and application prospects.

The invention provides a large plastic part recycling processing device and a processing method. The processing device comprises a fixing support, a drying tank, a vacuum pump, a hot-melting dicing device and a material storage tank, the middle of the fixing support is provided with an opening, and the two sides of the drying tank are each provided with a connecting convex table; and the drying tank is erected at the opening of the fixing support through the connecting convex tables at the two sides, the connecting convex tables are connected with the fixing support through fastening bolts, the bottom of the drying tank is provided with the hot-melting dicing device, the material storage tank is connected to the lower portion of the drying tank, the portions, at the outer side of the joint, of the drying tank and the material storage tank are connected through fastening bolts, the vacuum pump is connected to one side of the drying tank, and the vacuum pump is placed on the fixing support. The large plastic part recycling processing device has the beneficial effects that energy consumption is reduced, noise is small, crushed particles are regular, and the occupied area is small.

The invention relates to a method for preparing salinomycin sodium micro-granules, which comprises the steps of feed liquid preparation, spray drying and powder mixing packaging. A feed liquid is prepared by adding sodium hydroxide, calcium carbonate and a small quantity of carboxy methyl cellulose into a zymotic fluid to ensure that the pH value of the zymotic fluid is alkaline and contributes to the stability of the intermediate process and a final product, the feed liquid enters a spraygun through the pressure increase of a screw pump, and only a few seconds are spent to convert liquid drops into granules, thereby avoiding the pasting of materials and the production of substances containing toxic side effect at high temperature for a long time. The salinomycin sodium micro-granules prepared by the method are even and regular, thereby being mixed with feedstuff easily during the application, ensuring that animals have even and effective food intake, and avoiding the phenomenon that beasts and birds fail to intake or intake overmuch.