Polycation transgene vector and method for synthesizing same

A technology of transgenic carrier and polycation, which is applied in the field of polycation carrier and synthesis, can solve unrelated problems and achieve the effects of prolonging the action time, improving transfection efficiency, and tumor targeting effect

Inactive Publication Date: 2009-01-07
林李家宓 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many documents do not involve the cyclodextrin-polyethyleneimi...

Method used

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  • Polycation transgene vector and method for synthesizing same
  • Polycation transgene vector and method for synthesizing same
  • Polycation transgene vector and method for synthesizing same

Examples

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Embodiment 1

[0034] 1) Activation of cyclodextrin: take 1.9 grams of α-cyclodextrin, add 10 ml of dimethyl sulfoxide to dissolve it, and activate 10 grams of hydroxyl linker N, N'-disuccinimidyl sulfate Dissolve it in 20 ml of dimethyl sulfoxide, add the linking agent into the cyclodextrin solution in the dark under the protection of nitrogen, add 200 microliters of catalyst triethylamine, and stir for 120-240 minutes to react. After the reaction was completed, it was precipitated in refrigerated ether to obtain a white precipitate, which was filtered and dissolved in dimethyl sulfoxide for storage.

[0035] 2) Coupling of polyethyleneimine: take 2.5 grams of activated cyclodextrin, dissolve 9 grams of polyethyleneimine with a molecular weight of 600 in 20 ml of dimethyl sulfoxide, and protect the mixture from light under nitrogen protection. Add dropwise to the above-mentioned cyclodextrin solution, the dropping speed is slow, and the addition is completed in 90-120 minutes, and 300 micro...

Embodiment 2

[0038] 1) Activation of cyclodextrin: 2.1 g of β-cyclodextrin, dissolved in 15 ml of dimethyl sulfoxide, 15 g of linker N, N'-carbonyldiimidazole, which activates the hydroxyl group, was dissolved in 2 ml of dimethyl In the sulfoxide, add the linking agent into the cyclodextrin solution in the dark under the protection of nitrogen, add 300 microliters of catalyst triethylamine, and stir for 120-240 minutes to react. After the reaction was completed, it was precipitated in refrigerated ether to obtain a white precipitate, which was filtered and dissolved in dimethyl sulfoxide for storage.

[0039] 2) Coupling of polyethyleneimine: take 2.5 grams of activated cyclodextrin, dissolve 12 grams of polyethyleneimine with a molecular weight of 1200 in 20 ml of dimethyl sulfoxide, and remove it from light under nitrogen protection. Add dropwise to the cyclodextrin solution at a slow rate, and finish adding in 90-120 minutes, add 300 microliters of catalyst triethylamine, and continue t...

Embodiment 3

[0042] 1) Activation of cyclodextrin: 2.5 grams of γ-cyclodextrin, add 20 milliliters of dimethyl sulfoxide to dissolve it, and dissolve 20 grams of benzotriazole carbonate, a linker for activating hydroxyl groups, in 20 milliliters of dimethyl In the base sulfoxide, under the protection of nitrogen, add the linking agent into the cyclodextrin solution in the dark, add 150 microliters of catalyst triethylamine, and stir for 120-240 minutes to react. After the reaction was completed, it was precipitated in refrigerated ether to obtain a white precipitate, which was filtered and dissolved in dimethyl sulfoxide for storage.

[0043] 2) Coupling of polyethyleneimine: take 2.5 grams of activated cyclodextrin, dissolve 15 grams of polyethyleneimine with a molecular weight of 2000 in 30 milliliters of dimethyl sulfoxide, and put it dropwise under nitrogen protection in the dark. Add to the above cyclodextrin solution, the dropping speed is slow, and the addition is completed in 90-12...

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Abstract

A novel nano polycation transgenic vector relates to a nano polycation transgenic vector of a cyclodextrin-polyethyleneimine-folacin ternary assembly structure which takes the cyclodextrin as a skeleton material and polymerizes the cyclodextrin after activated with small molecular polyethyleneimine(PEI) as well as couples the decorative folacin on the cyclodextrin-polyethyleneimine to manufacture the nano polycation transgenic vector with tumor targeting. The material composing method of the vector is simple and effective with a high recycling rate; the material is shown to have the characteristics of low poison and high transfecting efficiency by the filtering of a plurality of tumor cell strains and experiences in the body of a rat.

Description

technical field [0001] The invention relates to a polycation transgene carrier, in particular to a polycation carrier and a synthesis method. Background technique [0002] Gene therapy is a new type of treatment that has emerged in the past 30 years. It introduces foreign epigenes into target cells and expresses them effectively, so as to achieve the purpose of treating diseases. In the gene drug delivery system, the research on the carrier has always been a hot research issue. Carriers carrying normal genes or therapeutic genes mainly include viral vectors and non-viral vectors. Viral vector-mediated systems have high gene transfection efficiency in vivo, but there are problems such as high cytotoxicity, low DNA carrying capacity, long preparation time and high price. Out of consideration for the safety of treatment, especially in 1999, the use of adenovirus vectors in the clinical trials of gene therapy in the United States led to the death of "Jesse Gelsinger", and the ...

Claims

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Application Information

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IPC IPC(8): C12N15/64C08B37/16
Inventor 林李家宓汤谷平姚宏孔祥复
Owner 林李家宓
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