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Composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and application thereof

A technology of microbial organic fertilizer and bacterial agent, which is applied in the direction of chemicals, biocides, and applications for biological control, and can solve problems such as reduced availability, low fertilizer utilization rate, and soil ecological environment deterioration

Active Publication Date: 2013-09-25
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, in the production of flue-cured tobacco in my country, there has been a phenomenon of emphasizing inorganic fertilizers and neglecting organic fertilizers. Due to the long-term large-scale application of chemical fertilizers and the decrease in the amount of organic fertilizers year by year, the soil ecological environment has deteriorated, the fertilizer utilization rate is low, and the quality of tobacco leaves has deteriorated. It has become one of the main limiting factors in today's quality-benefit flue-cured tobacco production

Method used

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  • Composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and application thereof
  • Composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and application thereof
  • Composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. The effect of the combination of Trichoderma reesei ACCC 30150 and Streptomyces silaceus ACCC 40021 on the growth and disease resistance of tobacco seedlings

[0054] In this example, tobacco was used as the test material, and two kinds of biocontrol agents, Trichoderma reesei ACCC 30150 and Streptomyces silaceus ACCC 40021, were studied by combining indoor antagonism test and in vivo screening test. The affinity and antibacterial broad-spectrum of the bacteria compound were analyzed, and the effects of the bio-control bacteria combination on the growth of tobacco seedlings, the activity of defense enzymes and the control effect of black shank were analyzed. The results showed that: (1) The two biocontrol strains had no mutual inhibitory effect and had high affinity, and the two strains and their metabolites were effective against pathogenic bacteria (black shank, damping-off and red spot) in different habitats. It has high antibacterial activity and broad an...

Embodiment 2

[0127] Embodiment 2, the preparation of tobacco disease prevention and growth-promoting bacterium agent and microbial organic fertilizer

[0128] 1. Fermentation of Trichoderma reesei ACCC 30150

[0129] Sporulation medium: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4 .7H 2 O 0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl 1.0g / L, MgSO 4 .7H 2 O 0.3g / L, corn stalk powder (through a 40mm sieve) 10g / L. PH 6.0. Sterilize at 115°C for 20 minutes.

[0130] Trichoderma reesei (Trichoderma reesei) ACCC 30150 was inoculated on a plate with PDA medium, and after 5 days of culture, 10 cultured cakes were punched out with a sterilized 0.5cm hole puncher, and transferred to the Put 100ml of culture solution in a 250ml Erlenmeyer flask (containing PDA liquid medium), put it in a shaker and culture at 30°C for 3 days. Then inoculate it into the above spore-forming medium according to the inoculum amount of 10% (volume percentage content), culture on a shaking table at 30°C (shak...

Embodiment 3

[0147] The solid microorganism organic fertilizer of embodiment 3, the tobacco disease prevention and growth promotion of embodiment 2 promotes tobacco growth

[0148] 1. Materials and methods

[0149] 1.1 Test site

[0150] The experiment was arranged in Shanzui Village, Youjun Town, Xichang City, Liangshan Prefecture, Sichuan Province, and selected representative soils with consistent crops, loose soil texture, flat plots, medium soil fertility (Table 8), convenient irrigation and drainage, and convenient transportation. Layout. The experimental field is managed by a tobacco farmer with good planting experience, and a soil testing and fertilization plan is adopted at the same time.

[0151] Table 8 Record form of basic soil fertility

[0152] soil fertility

[0153] 1.2 Test varieties

[0154] The variety tested was Yunyan 87, the main local variety, which was supplied by the company in Sichuan Province.

[0155] 1.3 Experimental design

[0156] Four treatmen...

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Abstract

The invention discloses a composite microbial fertilizer bactericide for disease prevention and growth promotion of tobacco and an application thereof. The bactericide can be used for promoting tobacco growth and / or preventing tobacco disease, and comprises active components namely Trichoderma ressei ACCC 30150 and Streptomyces silaceus ACCC 40021. The bactericide is environment-friendly, can inhibit dug resistance development of a disease pathogen, is favor of popularization of green food and organic agriculture, is low in cost and non-pollution, and safe to tobacco crops. By introducing antagonistic microbe in soil, through biological control, fungus and bacterial diseases can be effectively prevented, environment is effectively improved, agroecological system balance is maintained, and a foundation for sustainable development of tobacco can be laid.

Description

technical field [0001] The invention relates to a compound microbial fertilizer bacterial agent for tobacco disease prevention and growth promotion and its application. Background technique [0002] Tobacco is an important economic crop in China, and its quality is closely related to production efficiency. Tobacco varieties, climatic conditions, cultivation measures, soil, fertilization, and processing technology all have an important impact on the quality of flue-cured tobacco, and fertilization technology is one of the most important factors affecting the quality of flue-cured tobacco. For a long time, in the production of flue-cured tobacco in my country, there has been a phenomenon of emphasizing inorganic fertilizers and neglecting organic fertilizers. Due to the long-term large-scale application of chemical fertilizers and the decrease in the amount of organic fertilizers year by year, the soil ecological environment has deteriorated, the fertilizer utilization rate is...

Claims

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Application Information

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IPC IPC(8): A01N63/04C05F11/08A01P1/00A01P3/00A01G1/00
Inventor 顾金刚王倩李世贵张良刘好宝雷强屈健康
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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