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Method for preparing bean slags soluble food fibers by zymolysis method

A dietary fiber and soluble technology, which is applied in food preparation, food science, fungi, etc., can solve the problems of unsearched, unreported research, unsatisfactory research results of fermentation method, etc., to achieve increased content, simple processing process, The effect of mild conditions

Inactive Publication Date: 2008-04-16
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the research results of fermentation method preparation at home and abroad are not ideal at present.
[0010] At present, there is no report on the preparation of soluble dietary fiber from soybean dregs fermented by Trichoderma reesei abroad.
Moreover, no patents on the preparation of soluble dietary fiber from bean dregs by direct fermentation of microorganisms have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this embodiment, the okara is a by-product after producing soybean protein isolate. Fresh bean dregs were dried at 60°C for 14 hours, crushed through a 40-mesh sieve, and a fermentation medium was prepared at a ratio of bean dregs:water of 1:25, adjusted to pH 4, and sterilized at 121°C for 20 minutes. The Trichoderma reesei strain is a mutant strain selected by ultraviolet mutagenesis of Trichoderma reesei Rut C-30, and is a mutant strain suitable for growing in bean dregs and high-yielding cellulase and hemicellulase. The mutagenesis process is to put the spore suspension of Trichoderma reesei Rut C-30 under the irradiation of 30cm away from the ultraviolet lamp, and after mutagenesis for 40s, spread it on the cellulose selection medium for preliminary screening, and select the diameter ratio of the hydrolysis circle and the colony to be larger than the original one. The mutant strain of the strain Trichoderma reesei was inoculated in the bean dregs fermentation me...

Embodiment 2

[0023] In this example, after drying fresh bean dregs at 80°C for 12 hours, crush them through a 40-mesh sieve, prepare a fermentation medium according to the ratio of bean dregs:water of 1:20, adjust the pH to about 4.5, and sterilize at 121°C for 15 minutes . Then Trichoderma reesei was inoculated into the culture medium according to 4% of the liquid volume, and during the mutagenesis culture of Trichoderma reesei, shake flask fermentation culture at 29 DEG C for 106 hours reached the enzyme activity peak period to obtain the fermentation strain. Under the condition of 28°C, the culture medium inoculated with Trichoderma reesei was shaken at 160r / min for 3 days. Sterilize at 121°C and filter, concentrate the filtrate to half of the original volume, precipitate with absolute ethanol, and vacuum-dry the precipitate to obtain the finished product. The soluble dietary fiber content in the fermented bean dregs can reach 30% to 35% as determined by the AOAC method.

Embodiment 3

[0025]In this example, fresh bean dregs were dried at 70°C for 13 hours, crushed through a 40-mesh sieve, and a fermentation medium was prepared at a ratio of bean dregs:water of 1:22, adjusted to a pH of about 4.25, and sterilized at 121°C for 18 minutes. The strain is bred by ultraviolet mutagenesis. It is a mutant strain suitable for growing in bean dregs and high-yielding cellulase and hemicellulase. It reaches the peak of enzyme activity after 120 hours of shaking culture at 30°C. Cellulase and hemicellulase The activities reached 5.95U / ml and 127.1U / ml respectively. Use this mutant strain to ferment bean dregs, inoculate Trichoderma reesei into the medium at 3.5% of the liquid volume at 29°C, culture on a shaking table at 150r / min for 3.5 days, take it out, add water twice the volume of the bean dregs medium , sterilized at 121°C for 0.5h. Sterilize at 121°C and filter, concentrate the filtrate to half of the original volume, precipitate with absolute ethanol, and vacuu...

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PUM

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Abstract

The present invention provides a method for preparing bean dregs soluble dietary fiber by zymotechnics. Firstly, to dry the fresh bean dregs at the temperature of 60 to 80 DEG C for 12 to 14 hours, then to comminute until passing 40 mesh sieve, and to confect into ferment culture medium according to the ratio of 1 : 20 to 25 of bean dregs : water, and regulating the pH value to 4 to 4.5, then to disinfect at the temperature of 121 DEG C for 15 to 20 min to obtain liquid; to inoculate leeb wood mildew into the culture medium according to the 3 to 4 percent of the liquid volume ratio, then to shake bed culture at the speed of 140 to 160 r / min for 3 to 4d at the temperature of 28 to 30 DEG C. After sterilization, filtering and concentrating, to obtain deposit by non-water ethanol deposition, then to dry the deposit in vacuum to produce finished product. The present invention condition is geniality, equipment is simple, enzyme specificity is high, and can improve activity component amount maximum, has no pollution to circumstance, the SDF content in the bean dregs is increased greatly.

Description

(1) Technical field [0001] The invention relates to the food industry, in particular to a method for preparing soybean dregs soluble dietary fiber. (2) Background technology [0002] Dietary fiber is non-starch polysaccharides and anti-digestion oligosaccharides, which can be divided into soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) according to their solubility characteristics. The main components of insoluble dietary fiber are cellulose, some hemicellulose, and lignin; soluble dietary fiber includes the storage and secretion of certain plant cells and microbial polysaccharides, and its main component is gum. The main physiological functions of dietary fiber are intestinal adjustment, laxative, prevention and treatment of intestinal diseases and constipation; it can also regulate serum cholesterol, lower blood pressure, prevent and treat cardiovascular diseases; lower blood sugar, and prevent obesity. A large number of research results show that the solubi...

Claims

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Application Information

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IPC IPC(8): A23L1/202A23L1/308A23L1/09C12N1/14A23L33/22
Inventor 程建军李想江连洲朱秀清
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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