Trichoderma reesei cultivation method for improving yield of cellulase

A technology of Trichoderma reesei and cellulase, applied in the field of microbial cultivation in biochemistry, can solve the problems of inhibition, microbial growth and toxicity, etc., and achieve the effect of improving the enzyme activity of filter paper and shortening the enzyme production time

Active Publication Date: 2008-08-27
CHINA PETROLEUM & CHEM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after exceeding a certain limit value, dissolved oxygen is also toxic and inhibitory to microbial growth

Method used

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  • Trichoderma reesei cultivation method for improving yield of cellulase
  • Trichoderma reesei cultivation method for improving yield of cellulase
  • Trichoderma reesei cultivation method for improving yield of cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Trichoderma reesei fixed aeration and mass production of cellulase

[0023] Trichoderma reesei liquid activation medium is: 6g glucose, 0.6g peptone, 0.6mL Mandels trace elements, 60mL Mandels nutrient salt, 30mL citric acid buffer with a concentration of 1mol / L, add 510mL distilled water, and distribute them in a conical Sterilized in bottle.

[0024] Trichoderma reesei enzyme production medium is: glucose 6g, sulfate pulp 60g (dry weight), ammonium sulfate 13.2g, urea 3g, potassium dihydrogen phosphate 12g, calcium chloride 1.8g, magnesium sulfate 1.8g, 6mL of Mandels Trace elements, add water until the total liquid volume is 6L, and heat up to 100 ℃ for sterilization.

[0025] The Trichoderma reesei species was inserted into 50mL liquid activation medium, cultivated for 36h in a constant temperature oscillator at 26-32°C, 150-250r / min, and connected to a 10L fermenter at 1:10, with a liquid filling volume of 6L and ventilation. Cellulase is prepared by c...

Embodiment 2

[0029] Example 2: Trichoderma reesei regulates ventilation and mass-produces cellulase

[0030] Except for the ventilation rate, other conditions are the same as those in Example 1. The ventilation volume is controlled at 10vvh at the beginning, and the change of dissolved oxygen concentration is monitored. When the dissolved oxygen concentration drops to 20%, the ventilation volume is increased, and when the dissolved oxygen concentration rises to 30%, the ventilation volume is reduced. The dissolved oxygen concentration will rise in the later stage of enzyme production, and the ventilation volume should be reduced until it drops to 10vvh.

[0031] The oxygen and carbon dioxide concentrations in the exhaust gas were measured during the production of enzymes. image 3 shown. The highest carbon dioxide concentration in the exhaust gas is about 0.4%, which is only half of that of Example 1, which is beneficial to the growth and metabolism of mycelium.

[0032] The adjustment ...

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Abstract

The invention relates to a method for controlling the dissolved oxygen concentration of solution through adopting ventilation quantity regulation to increase the productivity of trichoderma reesei cellulase. The method is characterized in that the ventilation quantity is regulated in the enzyme production process to control the dissolved oxygen concentration in a main enzyme production stage to be 20%-30%, which not only enables the dissolved oxygen concentration of enzyme production liquid to be no lower than the critical concentration, but also lowers the carbon dioxide concentration in tail gas, and the method is beneficial for thalli to grow. Compared with the method for producing enzyme with fixed ventilation quantity, with the method of the invention for producing the enzyme, the filter paper enzyme activity can be increased by 30%, and the enzyme producing time can be shortened by 14%.

Description

1. Technical field [0001] The invention belongs to the technical field of microorganism culture in biochemistry, and particularly relates to a Trichoderma reesei culture method for improving cellulase yield. 2. Background technology [0002] As the world's oil reserves are depleted, ethanol has become one of the most important alternatives to liquid fuels. Vigorously developing new renewable energy sources is a prerequisite for China to maintain sustainable development in the 21st century. Ethanol is a renewable energy produced by sugar fermentation. Traditional ethanol fermentation uses grain as raw material, but it has become increasingly unrealistic to rely on grain to produce fuel ethanol for a long time. Plant cellulose is the most abundant renewable resource on earth. It will be the best raw material for ethanol production in the future. Cellulose is a linear polymer linked by β-1,4-glycosidic bonds, and its degradation into monosaccharides is the key to further ferm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/885
Inventor 余世袁勇强杭志喜
Owner CHINA PETROLEUM & CHEM CORP
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